EB病毒感染与XRCC1-Arg399Gln基因多态性在鼻咽癌发生中的交互作用

发布时间：2018-03-27 16:00

本文选题：XRCC　切入点：鼻咽癌　出处：《实验与检验医学》2016年05期

【摘要】：目的评估XRCCl-Arg399 Gln单核苷酸多态性在鼻咽癌发病中的风险,研究XRCCl-Arg399 Gln单核苷酸多态性与EB病毒感染的交互作用。方法采用病例对照研究,收集90名经病理确诊的鼻咽癌患者作为病例组和75名在我院进行健康体检的病人作为对照,并按照性别和年龄1∶1配对,提取全血基因组DNA进行PCR扩增,对扩增产物再进行测序PCR反应,最后分析得出XRCC1 Arg399Gln的基因型。结果 EB病毒感染能增加鼻咽癌发病风险,VCA-IgA、EA-IgA和Rta-IgG抗体三项阳性时可以增加鼻咽癌发病风险,但单项VCA-IgA阳性是鼻咽癌发病的最强危险因素(OR=26.526,95%CI:11.190~62.884),单因素条件Logistic回归分析显示,与携带XRCC-1 Arg399Gln的AA基因型相比,携带XRCC-1 Arg399Gln的GG基因型可增加鼻咽癌发病风险(OR=4.3,95%CI:1.184~15.618);XRCC-1 Arg399Gln的GA基因型与VCAIgA存在交互作用(OR=20.755,95%CI:5.312~81.094)。结论 XRCC-1基因的Arg399Gln基因多态性能够增加患鼻咽癌的风险,且Arg399Gln的GA基因型与EB病毒感染具有一定的交互作用。
[Abstract]:Objective to evaluate the risk of XRCCl-Arg399 Gln single nucleotide polymorphism (SNP) in nasopharyngeal carcinoma (NPC) and to study the interaction between XRCCl-Arg399 Gln SNP and EBV infection. Ninety patients with nasopharyngeal carcinoma (NPC) confirmed by pathology were selected as case group and 75 patients who underwent physical examination in our hospital as controls. Genomic DNA was extracted from whole blood for PCR amplification according to sex and age 1:1. The amplified products were sequenced by PCR reaction, and the genotypes of XRCC1 Arg399Gln were analyzed. Results Epstein-Barr virus infection could increase the risk of nasopharyngeal carcinoma when VCA-IgAEA-IgA and Rta-IgG antibodies were positive. However, the single VCA-IgA positive was the strongest risk factor for the development of nasopharyngeal carcinoma (NPC). The single factor Logistic regression analysis showed that compared with the AA genotype carrying XRCC-1 Arg399Gln, CI: 11.190 ~ 62.884%. The GG genotype carrying XRCC-1 Arg399Gln can increase the risk of nasopharyngeal carcinoma (NPC) by 4.395% CI: 1.1844% 15.618% XRCC-1 Arg399Gln and VCAIgA. Conclusion the Arg399Gln polymorphism of XRCC-1 gene can increase the risk of NPC. The GA genotype of Arg399Gln has some interaction with EB virus infection.
【作者单位】：福建医科大学附属肿瘤医院福建省肿瘤转化医学重点实验室福建省肿瘤医院检验科;
【基金】：福建省自然科学基金资助项目(2014J01297) 福建省卫生厅青年科研课题(2012-2-12)
【分类号】：R739.63

【相似文献】