CXCR4和MMP-13在喉鳞癌中的表达及其在肿瘤浸润转移机制中的作用

发布时间：2018-03-29 18:45

本文选题：CXCR4　切入点：MMP-13　出处：《山西医科大学》2012年硕士论文

【摘要】：目的：探讨趋化生长因子受体4(CXCR4)及基质金属蛋白酶-13(MMP-13)在喉鳞状细胞癌中的表达,及其与肿瘤浸润转移的关系,并研究其临床意义。方法：采用免疫组织化学方法检测70例喉癌组织及10例癌旁正常组织CXCR4和MMP-13蛋白的表达情况,用逆转录-聚合酶链式反应技术检测30例喉癌组织及10例癌旁正常组织中CXCR4和MMP-13的表达情况,并对喉癌组织按年龄、性别、临床分期、肿瘤分化程度、淋巴结转移进行分组,分别用两种方法探讨二者与各临床病理参数及肿瘤浸润转移的关系。结果：免疫组化和RT-PCR结果均显示：CXCR4和MMP-13在喉癌组织中呈过度表达,而自身正常喉黏膜组织中弱表达或不表达,其差异具有统计学意义(PO.05),二者均明显高于正常组织；在肿瘤组织中,CXCR4的表达与患者的年龄、性别无关(PO.05),而与临床分期、肿瘤分化程度、淋巴结转移相关(PO.05)；同时还显示：MMP-13的过度表达与肿瘤分化程度、淋巴结转移相关(PO.05),而与患者的年龄、性别、临床分期无关。蛋白表达水平情况与基因表达水平基本相一致。CXCR4和MMP-13在喉鳞状细胞癌的蛋白及mRNA表达水平呈显著正相关。结论：1、CXCR4和MMP-13可能参与了喉鳞状细胞癌的发生,在喉鳞癌的侵袭和转移中发挥着重要作用。2、联合检测CXCR4和MMP-13有望成为喉鳞状细胞癌早期诊断和预后判断的生物学指标。3、CXCR4和MMP-13存在相关性,两者可能存在上调关系。
[Abstract]:Objective: to investigate the expression of chemokine growth factor receptor (CXCR4) and matrix metalloproteinase-13 (MMP-13) in laryngeal squamous cell carcinoma (LSCC) and its relationship with tumor invasion and metastasis. Methods: immunohistochemical method was used to detect the expression of CXCR4 and MMP-13 protein in 70 cases of laryngeal carcinoma and 10 cases of adjacent normal tissues. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of CXCR4 and MMP-13 in 30 cases of laryngeal carcinoma and 10 cases of adjacent normal tissues. The tissues were grouped according to age, sex, clinical stage, tumor differentiation and lymph node metastasis. The relationship between the two methods and the clinicopathological parameters and tumor invasion and metastasis were studied. Results: the results of immunohistochemistry and RT-PCR showed that the expression of MMP-13 and CXCR4 were overexpressed in laryngeal carcinoma, but the expression was weak or not in normal laryngeal mucosa. The difference was statistically significant (P < 0.05). Both of them were significantly higher than those in normal tissues. The expression of CXCR4 was not related to the age and sex of the patients, but to clinical stage, tumor differentiation, lymph node metastasis, and it also showed that the overexpression of MMP 13 was related to the degree of tumor differentiation. Lymph node metastasis was associated with PO.05, but not with age, sex and clinical stage. The expression of protein was basically consistent with the expression of gene. CXCR4 and MMP-13 were positively correlated with the expression of protein and mRNA in laryngeal squamous cell carcinoma (LSCC). ConclusionCXCR4 and MMP-13 may be involved in the development of laryngeal squamous cell carcinoma. Combined detection of CXCR4 and MMP-13 is expected to be a biological marker for early diagnosis and prognosis of laryngeal squamous cell carcinoma. There is a correlation between CXCR4 and MMP-13, which may be up-regulated.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.65

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