Ngn2和Math6在小鼠内耳不同时期表达的初步研究

发布时间：2018-04-05 19:13

本文选题：bHLH　切入点：neurogenin　出处：《中华耳科学杂志》2012年01期

【摘要】：目的研究neurogenin2(ngn2)和math6在不同时期小鼠内耳的表达变化,并初步探讨其对螺旋神经元发育的调控作用。方法本实验选取不同时期健康的BALB/c小鼠,以E12.5、E13.5、E14.5、E17.5、P1、P7和成年为观察时间点,应用real-time PCR、免疫组织化学方法检测ngn2、math6在耳蜗中mRNA和蛋白的表达。结果 (1)re al-time PCR结果提示ngn2在E12.5d呈高表达,随后在E13.5d表达量下调,直至成年呈低水平表达;math6在E12.5-P1都呈较高水平表达,其中在P1达到高峰,随后于P7至成年表达量下调。(2)免疫组织化学结果提示,在E13.5、E14.5和P1,ngn2和math6在螺旋神经节处(或螺旋神经元细胞核处)有不同程度的阳性表达;在成年期,math6在细胞核有弱表达,但ngn2几乎不表达,各组间差异有统计学意义(P0.05)。结论 (1)ngn2在E12.5d表达呈高峰,提示其在有增殖能力的神经祖细胞中起作用;math6在E12.5d-P1呈高表达,提示其在神经祖细胞的增殖以及分化中其起重要作用;(2)ngn2和math6在耳蜗中表达的空间和时间模式的重叠和差异,提示它们在调控通路中可能受到共同的分子调控。
[Abstract]:Objective to study the changes of neurogenin 2 ngn2) and math6 expression in the inner ear of mice at different stages, and to explore the regulation of neurogenin 2 ngn2) and math6 on the development of spiral neurons.Methods in this experiment, healthy BALB/c mice in different periods were selected. The expression of mRNA and protein in cochlea was detected by real-time and immunohistochemical method with E12.5E13.5E14.5E14.5E17.5P1P7 and adult as the observation time points. The expression of ngn2mmath6 in the cochlea was detected by immunohistochemical method, and the expression of ngn2mmath6 in the cochlea was detected by immunohistochemistry.Results the results of al-time PCR showed that the expression of ngn2 was high on day 12.5, then down-regulated at day E13.5. until the adult showed a low level of expression of simath6 in E12.5-P1, there was a high level of ngn2 expression in E12.5-P1, and reached its peak at P1.The results of immunohistochemistry showed that there were different degrees of positive expression in the helical ganglion (or the nucleus of the spiral neuron) in E13.5N 14.5, P1ngn2 and math6, and in the nucleus of the spiral neuron in the adult, and in the nucleus of the spiral ganglion, the expression of mafth6 was weakly expressed in the nucleus of adult.However, the expression of ngn2 was almost non-expressed, and the difference was statistically significant (P 0.05).Conclusion the peak expression of ngn2 in E12.5 indicates that it plays an important role in the proliferation of neural progenitor cells and the expression of ngn2 is highly expressed in E12.5d-P1.The results suggest that they play an important role in the proliferation and differentiation of neural progenitor cells. The spatial and temporal patterns of expression of ngn2 and math6 in cochlea may be regulated by common molecules.
【作者单位】：第四军医大学西京医院耳鼻咽喉-头颈外科;
【基金】：国家自然科学基金重点项目(N0:30930098)
【分类号】：R764

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