银杏叶提取物对慢性间歇性低氧大鼠胸主动脉重构的影响

发布时间：2018-04-11 00:25

本文选题：银杏叶提取物 + p38MAPK　；参考：《桂林医学院》2017年硕士论文

【摘要】：目的:探索银杏叶提取物对慢性间歇性低氧大鼠胸主动脉血管重构的作用。方法:选取30只健康、雄性Wistar大鼠,随机分为正常对照组(NC组)、慢性间歇性低氧组(CIH组)、慢性间歇低氧联合银杏叶提取物灌胃组(CIH+GBE组),各组10只。HE染色法检测各试验大鼠胸主动脉血管的病理变化;用荧光定量PCR(qRTPCR)方法检测胸主动脉p38MAPK、ERK1/2、JNK的mRNA表达水平;用Western blot方法检测上述三个因子蛋白表达水平及p-ERK1/2的水平;用免疫酶联反应法检测大鼠血清中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、超敏C反应蛋白(hsCRP)的含量。结果:(1)HE染色病理结果显示:与NC组相比CIH组胸主动脉出现重构,胸主动脉见中膜厚度增加、中膜厚度与管腔内径之比增大(P0.05),CIH+GBE组与CIH组相比,上述两指标均降低(P0.05),Masson染色显示:与NC组相比CIH组胸主动脉胶原纤维化面积明显增加(P0.05),而CIH+GBE组与CIH组相比,上述指标均降低(P0.05);(2)qRT-PCR检测结果显示:与NC组比较IH组胸主动脉中p38MAPK、JNK的mRNA转录水平明显增强(P0.05),CIH+GBE与IH组比较发现CIH+GBE组p38MAPK、JNK的mRNA变化趋势与上一组比较相反(P0.05),而ERK1/2的转录水平在上述两组中比较无明显变化(P0.05);(3)Westen blot检查显示:各组p38MAPK、JNK、总ERK1/2蛋白变化趋势与qRTPCR检查结果一致,而与NC组相比CIH组ERK1/2磷酸化明显升高(P0.05),CIH+GBE组与CIH组比较,CIH+GBE组ERK1/2磷酸化水平明显降低(P0.05);(4)ELISA检测结果显示:与NC组相比,CIH组大鼠血清中TNF-α、IL-6、hsCRP因子的水平均显著上升(P0.05),而CIH+GBE组与CIH组比较,大鼠血清中TNF-α、hsCRP的含量明显降低(P0.05),IL-6有所降低,但无统计学意义(P0.05)。结论:间歇性低氧可以引起大鼠胸主动脉血管重构;银杏叶提取物可以抑制间歇性缺氧大鼠胸主动脉血管ERK1/2蛋白磷酸化,降低大鼠主动脉表达JNK、p38MAPK,并降低其血清中TNF-α、hsCRP的水平。
[Abstract]:Objective: to investigate the effect of ginkgo biloba extract on vascular remodeling of thoracic aorta in chronic intermittent hypoxia rats.Methods: 30 healthy male Wistar rats were selected.They were randomly divided into normal control group (n = 10), chronic intermittent hypoxia group (n = 10), chronic intermittent hypoxia group (n = 10), chronic intermittent hypoxia group (n = 10), chronic intermittent hypoxia group (n = 10) and ginkgo biloba extract (Ginkgo biloba extract) group (n = 10).The expression of mRNA in the thoracic aorta p38 MAPKG ERK1 / 2 JNK was detected by fluorescence quantitative PCRQ RT PCR, and the expression levels of the above three factors and p-ERK1/2 were detected by Western blot method.The levels of tumor necrosis factor- 伪 (TNF- 伪), interleukin-6 (IL-6) and hypersensitive C-reactive protein (hsCRP) in the serum of rats were detected by immuno-enzyme linked reaction (Elisa).Results compared with NC group, there were remodeling of thoracic aorta, increase of medial thickness of thoracic aorta and increase of ratio of medial thickness to lumen diameter in CIH group compared with CIH group.Compared with NC group, the area of collagen fibrosis of thoracic aorta in CIH group was significantly increased, while that in CIH GBE group was significantly higher than that in CIH group, and that in CIH GBE group was significantly higher than that in CIH group.The results of RT-PCR showed that compared with NC group, the mRNA transcription level of p38MAPK-1 JNK in thoracic aorta of IH group was significantly enhanced than that of IH group and IH group. It was found that the mRNA change trend of p38MAPKN JNK in CIH GBE group was opposite to that in the previous group (P0.05), but ERK1/2 translocation in IH group was different from that in the previous group.There was no significant change in the recorded level in the above two groups. The results of blot showed that the change trend of total ERK1/2 protein in each group was consistent with that of qRTPCR.CIH GBE group was compared with CIH group.The content of TNF- 伪 hsCRP in the serum of rats was significantly decreased, but the level of IL-6 was decreased, but there was no significant difference in serum TNF- 伪 hsCRP.Conclusion: intermittent hypoxia can induce vascular remodeling of thoracic aorta in rats, Ginkgo biloba extract can inhibit the phosphorylation of ERK1/2 protein in thoracic aorta of rats with intermittent hypoxia, decrease the expression of ERK1/2 p38 MAPK in rat aorta, and decrease the level of TNF- 伪 hsCRP in serum.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R766

【参考文献】