猪小肠黏膜下层脱细胞支架的制备及组织学评价

发布时间：2018-04-15 05:26

本文选题：组织工程 + 支架材料　；参考：《解剖学报》2016年06期

【摘要】：目的评价不同浓度的十二烷基磺酸钠(SDS)处理猪小肠黏膜下层(SIS)的脱细胞效果,筛选最佳脱细胞浓度,为组织工程化角膜上皮的制备提供支架材料。方法配制0.1%、0.2%、0.3%、0.5%SDS随机分成A、B、C、D 4组,分别脱细胞处理SIS 15min、30min、1h、2h(n=20),同时观察SIS的大体形态变化,HE和4,6-二脒基-2-苯基吲哚(DAPI)染色观察脱细胞前后SIS的生物学特性,并对脱细胞支架进行基因组DNA分析(n=5)。结果脱细胞SIS肉眼观察呈乳白色、半透明的膜状物,具有一定的弹性、韧性及透光性;HE和DAPI染色光学显微镜观察结果显示,A组及B组处理30min时SIS生物支架无细胞及DNA残留,组织结构保留完整,胶原纤维间孔隙率增加;A组及B组处理30min脱细胞率可达90%以上。结论 0.1%及0.2%SDS处理30min条件下脱细胞效果较好,能更好地降低SIS的免疫原性,是SDS脱细胞处理SIS比较理想的浓度时间条件,为组织工程化角膜上皮的构建奠定理论基础。
[Abstract]:Objective to evaluate the acellular effect of different concentrations of sodium dodecyl sulfonate (SDS) on porcine intestinal submucous membrane (SISs) and to select the best concentration of acellular for the preparation of tissue engineered corneal epithelium.Methods A group of 0. 3 and 0. 3 were randomly divided into two groups: group D, treated with acellular SIS for 15 min and 30 min for 1 hour, respectively. The gross morphological changes of SIS were observed and the biological characteristics of SIS before and after acellular treatment were observed.The genomic DNA analysis of acellular scaffolds was carried out.Results the naked eye of acellular SIS showed milky white, translucent membrane with certain elasticity, toughness and transmittance. The results of HE and DAPI staining optical microscope showed that there were no cells and DNA residues in SIS scaffolds treated with 30min in group A and group B.The tissue structure remained intact, the porosity between collagen fibers increased and the acellular rate of 30min in group A and group B increased to more than 90%.Conclusion the acellular effect of 0.1% and 0.2%SDS treatment on 30min is better, and the immunogenicity of SIS can be reduced better. It is an ideal concentration time condition for SIS treated with SDS acellular, and lays a theoretical foundation for the construction of tissue-engineered corneal epithelium.
【作者单位】：山东大学齐鲁医院眼科;
【基金】：山东省自然科学基金面上项目(ZR2015HM050) 山东省优秀中青年科学家科研奖励基金(BS2011YY014)
【分类号】：R779.6

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