MIF和NF-κB及IL-1β在鼻息肉组织中的表达及意义

发布时间：2018-04-19 04:06

本文选题：鼻息肉 + 巨噬细胞移动抑制因子　；参考：《临床耳鼻咽喉头颈外科杂志》2016年03期

【摘要】：目的:通过检测巨噬细胞移动抑制因子(MIF)、核转录因子-κB(NF-κB)、IL-1β在鼻息肉组织及正常下鼻甲组织中的表达,并分析三者的相关性,探讨其在鼻息肉中的作用,并分析可能的作用机制。方法:应用苏木精-伊红染色方法分析48例鼻息肉患者(鼻息肉组)EOS等炎性细胞浸润的情况;采取免疫组织化学法检测MIF、NF-κB p65及IL-1β的表达及分布,以21例鼻中隔矫正术中下鼻甲黏膜为对照组;应用VAS、Lund-Kennedy方法对鼻息肉组进行病情分析,并对鼻息肉病情严重程度与MIF、NF-κB p65及IL-1β的表达水平进行相关性分析。结果:1鼻息肉组的EOS浸润程度、MIF、NF-κB p65、IL-1β水平均明显高于对照组(均P0.05);MIF、NF-κB p65、IL-1β之间呈正相关(r分别为0.74、0.66、0.60,均P0.05);NF-κB p65核染阳性率与MIF、IL-1β均存在显著正相关性(r分别为0.67、0.63,均P0.05);EOS浸润程度与MIF、IL-1β存在正相关(r分别为0.49、0.55,P0.05),与NF-κB p65及NF-κB p65核染阳性率无明显相关性(P0.05);2鼻息肉组VAS评分为8.24±1.72,鼻内镜检查评分为8.63±3.81。Spearman相关性分析显示,VAS评分与MIF水平呈正相关(r=0.71,P0.05),与NF-κB p65、NF-κB p65核染阳性率以及IL-1β均无明显相关性(P0.05);鼻内镜检查评分与MIF及NF-κB p65核染阳性率呈正相关(r分别为0.79、0.73,P0.05),与NF-κB p65及IL-1β水平无明显相关性(P0.05)。结论:MIF、NF-κB p65及IL-1β可能通过协同作用促进鼻息肉的发生发展,鼻息肉中可能存在有IL-1β-NF-κB-MIF途径;MIF与NF-κB p65可能参与维持下鼻甲的生理功能或与下鼻甲最轻持续炎症反应有关系,MIF及NF-κB p65核染阳性率在一定程度上可作为判断鼻息肉严重程度及预后的一个标准。
[Abstract]:Objective: to detect the expression of macrophage migration inhibitory factor (MIF) and nuclear transcription factor (NF- 魏 B)-魏-魏 B (NF- 魏 B) IL-1 尾 in nasal polyps and normal inferior turbinate tissues, and to analyze the correlation between them, and to explore the role of them in nasal polyps and the possible mechanism.Methods: the infiltration of inflammatory cells such as EOS in 48 patients with nasal polyps was analyzed by hematoxylin-eosin staining, and the expression and distribution of MIF- 魏 B p65 and IL-1 尾 were detected by immunohistochemical method.21 cases of nasal septum correction were used as control group, and the severity of nasal polyps was analyzed by using VAS-Lund-Kennedy method, and the correlation between the severity of nasal polyps and the expression of MIF-NF- 魏 B p65 and IL-1 尾 was analyzed.Results the levels of IL-1 尾 in the EOS infiltrating degree and p65- 魏 B of the nasal polyp group were significantly higher than those in the control group (P 0.05). The positive correlation between IL-1 尾 and p65 尾 in the nasal polyp group was 0.744 ~ 0.660.60, respectively. There was a significant positive correlation between the positive rate of nuclear staining of NF- 魏 B p65 and the IL-1 尾 of MIF- 魏 B, and the infiltration degree of P0.05- 魏 B was 0.670.63, and the positive rate of p65- 魏 B p65 in the control group was 0.670.63, respectively. The positive correlation between the positive rate of nuclear staining of NF- 魏 B p65 and the infiltration degree of IL-1 尾 was 0.670.63, respectively.The positive correlation between IL 1 尾 and MIFK 尾 was 0.49 卤0.55U P 0.05, respectively. There was no significant correlation with the positive rate of nuclear staining of NF- 魏 B p65 and NF- 魏 B p65. The VAS score of nasal polyp group was 8.24 卤1.72, and the nasal endoscopy score was 8.63 卤3.81.Spearman correlation analysis showed that VAS score had a positive correlation with MIF level, and had a positive correlation with NF- 魏 B p65 and NF- 魏 B p65NF- 魏 B level.There was no significant correlation between the positive rate of p65 and IL-1 尾 and the positive rate of MIF and NF- 魏 B p65 in nasal endoscopy, but there was no significant correlation between the positive rate of p65 and NF- 魏 B p65 and the level of NF- 魏 B p65 and IL-1 尾.Conclusion it is suggested that NF- 魏 B p65 and IL-1 尾 may promote the development of nasal polyps through synergistic action.The IL-1 尾 -NF- 魏 B-MIF pathway and NF- 魏 B p65 may be involved in maintaining the physiological function of inferior turbinate or may be related to the minimal sustained inflammatory response of inferior turbinate. The positive rate of nuclear staining of MIF and NF- 魏 B p65 may be used to judge nasal polyps to some extent.A criterion for severity and prognosis.
【作者单位】：郑州大学第一附属医院鼻科;
【分类号】：R765.25

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