超声微泡造影剂联合美金胺增强视神经损伤大鼠视网膜神经节细胞保护作用

发布时间：2018-04-22 23:27

本文选题：视神经损伤 + 视网膜神经节细胞　；参考：《重庆医科大学》2012年硕士论文

【摘要】：目的探讨超声微泡造影剂联合美金胺(memantine),对由视神经钳夹造成损伤的大鼠视网膜神经节细胞（RGC）的保护作用。方法将40只SD雄性成年大鼠按随机分组法分为以下5个处理组: A组：正常对照组，B组：假手术组，C组：空白对照组，D组：玻璃体腔单独注射美金胺组，E组：玻璃体腔注射美金胺加超声微泡组，每组大鼠的数量均为8只，各组再按观察时间点的不同，随机分为:视神经损伤后第1周和第2周2个亚组，各亚组的大鼠数量均为4只。A组不做任何处理；B组只暴露视神经，不进行钳夹，玻璃体腔注射生理盐水，立即用超声波辐照大鼠眼球；C～E组建立视神经钳夹伤模型后，处理方式分别为C组玻璃体腔注射生理盐水，D组玻璃体腔注射美金胺，E组玻璃体腔注射超声微泡造影剂，再以相同方法玻璃体腔注射美金胺，之后立即用超声波辐照大鼠眼球。视神经钳夹后第1、第2周时，A～E组分别用荧光金（FG）逆行标记RGC并计数；闪光视觉诱发电位（F-VEP）检测，记录P_(100)波潜伏期及振幅；荧光电子显微镜下观察视网膜细胞形态学改变。结果逆行荧光金(FG)标记RGC结果显示，各处理组视网膜定向铺片上均可见金黄色着染的RGC。A、B组间RGC数差异没有统计学意义(q=0.018，，0.011；P=0.986，0.873)；C～E组RGC数均较A组减少，差异具有统计学意义（F=85.944，P=0.012）；D组RGC数多于C组，差异具有统计学意义（q=1.721,1.924；P=0.043,0.037）；E组RGC数明显高于C、D组，差异具有统计学意义（q=1.128,1.482，P=0.027,0.008；q=1.453,1.855，P=0.031,0.010）。F-VEP检测发现，A、B组间P100波潜伏期及振幅差异没有统计学意义（q=0.008，0.019，P=0.981,0.946；q=0.072,0.052，P=0.737,0.851）；C～E组P100波潜伏期较A组延长，振幅较A组降低，差异具有统计学意义（F=134.312，F=106.312，P=0.017,0.009）。荧光电子显微镜下观察发现，A、B组大鼠视网膜各层结构完整，排列整齐，RGC排列紧密整齐，细胞核均匀深染，胞核大小一致。C～E组大鼠的视网膜不同程度的水肿变厚，RGC有不同程度的排列紊乱，空泡化及细胞数目的减少。结论超声微泡造影剂联合美金胺能抑制视神经损伤后大鼠RGC的丢失，促进其视功能的恢复，对视神经损伤大鼠的RGC具有保护作用。
[Abstract]:Objective to investigate the protective effect of ultrasound microbubble contrast agent combined with medeline memantine on retinal ganglion cells (RGC) injured by optic forceps in rats. Methods Forty Sprague-Dawley male adult rats were randomly divided into the following five groups: group A: normal control group: group B: sham operation group: group C: blank control group: group D: intravitreous injection of mebamine alone: group E; Vitreous injection of mebamine plus ultrasound microbubble group, The number of rats in each group was 8. Each group was randomly divided into two subgroups according to the observed time points: the first week and the second week after optic nerve injury. The number of rats in each subgroup was 4 rats in group A and only exposed to optic nerve in group B without any treatment. Without clamp, normal saline was injected into the vitreous cavity, and the model of optic nerve clamp injury was established in group C (C) E by ultrasonic irradiation. The methods of treatment were as follows: group C was injected with normal saline and group D was injected with menamine E group by intravitreal injection of ultrasound microbubble contrast agent, and then intravitreous injection of mebamine was used in the same way, and then the eyeball of rats was irradiated with ultrasound immediately. In the first and second week after optic forceps clamp, the RGC was labeled with FG retrograde and counted, the latency and amplitude of the wave were recorded by flash visual evoked potential (F-VEP), and the morphological changes of retinal cells were observed under fluorescence electron microscope. Results the results of RGC labeled with retrograde FG showed that the RGC number of group B with golden yellow staining was not significantly different from that of group A (P 0.9860.873C), and the number of RGC in group E was lower than that in group A. The number of RGC in group D was significantly higher than that in group C, and the number of RGC in group E was significantly higher than that in group C. The results of fluorescence electron microscopy showed that all layers of retina in group A were intact, and RGC was arranged closely, and the nucleus was homogeneous and deep stained. The retinal edema and thickening of RGC of different degrees in the same size of nucleus. The RGC had different degree of arrangement disorder, vacuolation and the decrease of the number of cells. Conclusion Ultrasonic microbubble contrast agent combined with mebamine can inhibit the loss of RGC and promote the recovery of visual function in rats with optic nerve injury. It has a protective effect on RGC in rats with optic nerve injury.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R774.1

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