临床喉鳞癌组织标本中G3BP和CD44v6的检测及意义

发布时间：2018-04-26 02:34

  本文选题：喉鳞癌 + GAP酶激活蛋白SH功能区结合蛋白(GBP)　； 参考：《细胞与分子免疫学杂志》2017年07期

【摘要】：目的研究RNA结合的Ras-GAP酶激活蛋白SH3结合蛋白(G3BP)、肿瘤干细胞标志物CD44v6蛋白与喉鳞状细胞癌(喉鳞癌)发生发展的关系,探讨G3BP、CD44v6蛋白与血管新生的相关性。方法收集56例喉鳞癌患者癌组织及对应癌旁组织,采用Western blot法检测喉鳞癌患者癌组织及对应癌旁组织中G3BP、CD44v6蛋白水平,使用免疫组织化学染色法分别检测G3BP、CD44v6、血管内皮生长因子A(VEGF-A)的表达,并分析G3BP、CD44v6表达与喉鳞癌患者临床病理特征的关系及与VEGF-A表达的相关性;采用Ⅷ因子相关抗原(FⅧAg)免疫组织化学染色确定微血管密度(MVD),观察G3BP、CD44v6表达与MVD之间的关系。结果喉鳞癌患者癌组织中G3BP蛋白、CD44v6蛋白水平均明显高于对应癌旁的正常组织。喉鳞癌患者癌组织中的G3BP、CD44v6、VEGF-A表达阳性率分别为58.9%、53.6%、46.4%,均明显高于对应的癌旁正常组织。喉鳞癌患者癌组织中G3BP、CD44v6蛋白表达阳性率与临床分期、复发或转移及淋巴结转移相关,但与年龄和肿瘤大小无关。经Pearson相关性分析,喉鳞癌患者癌组织中G3BP、CD44v6蛋白的表达与VEGF-A表达呈正相关(r=0.741,r=0.756);喉鳞癌患者癌组织中G3BP与CD44v6蛋白阳性表达病例的MVD值均明显高于癌旁正常组织中MVD值;癌组织中G3BP与CD44v6蛋白阴性表达病例MVD值与癌旁正常组织中MVD值比较无明显差异。结论喉鳞癌组织G3BP、CD44v6蛋白高表达,可能通过促进VEGF-A的表达来诱发肿瘤微血管的新生,促进喉鳞癌侵袭、转移。
[Abstract]:Objective to study the relationship between RNA binding Ras-GAP activator protein SH3 binding protein G3BP1, tumor stem cell marker CD44v6 protein and laryngeal squamous cell carcinoma (LSCC), and to explore the relationship between G3BP- CD44v6 protein and angiogenesis in laryngeal squamous cell carcinoma (LSCC). Methods A total of 56 cases of laryngeal squamous cell carcinoma (LSCC) and its adjacent tissues were collected. The levels of G3BP- CD44v6 protein in laryngeal squamous cell carcinoma (LSCC) tissues and adjacent tissues were detected by Western blot assay. The expression of CD44v6 and VEGF-A were detected by immunohistochemical staining. The relationship between the expression of CD44v6 and VEGF-A in laryngeal squamous cell carcinoma (LSCC) was analyzed. The microvessel density (MVD) was determined by immunohistochemical staining of F 鈪，

本文编号：1804111