玻璃体腔注射塞来昔布对大鼠氧诱导视网膜病变模型新生血管的抑制作用及其机制

发布时间：2018-04-27 06:18

本文选题：塞来昔布 + 氧诱导视网膜病变　；参考：《石河子大学》2017年硕士论文

【摘要】：目的通过对大鼠氧诱导视网膜病变(Oxygen-induced retinopathy,OIR)模型进行玻璃体腔注射塞来昔布,探讨塞来昔布在OIR中对视网膜新生血管的作用及其机制。方法7d龄SD乳鼠96只随机分为6组:Z组:正常组;O组:OIR组;A组:OIR+溶媒对照组;B组:OIR+塞来昔布5μg组;C组:OIR+塞来昔布20μg组;D组:OIR+塞来昔布80μg组。除Z组在正常环境饲养外,其余各组乳鼠均建立OIR模型。乳鼠于生后第12天出氧箱后给予玻璃体内注射DMSO及相应剂量的塞来昔布,于生后第17天处死,做组织切片HE染色观察视网膜组织形态并计数突破内界膜的血管内皮细胞核数;石蜡切片行免疫组化染色检测VEGF蛋白的表达。结果HE染色显示,Z组、O组、A组、B组、C组、D组突破视网膜内界膜的血管内皮细胞核数分别为(0.44±0.18)个、(30.60±5.36)个、(28.05±4.68)个、(19.58±4.58)个、(10.13±1.93)个、(7.58±2.68)个;除O组与A组外,各组间差异均有统计学意义(均为P0.05),经塞来昔布治疗后,突破内界膜的血管内皮细胞核明显减少,且与剂量正相关;免疫组化结果显示,Z组VEGF蛋白表达呈阴性,表达率为10%,其余各组可见VEGF蛋白的阳性表达,O、A组阳性表达较高,阳性率分别为90%、86%,表现为棕褐色颗粒或团块,均高于B组、C组、D组,且后三组的阳性表达依次减低为68%、42%、30%。结论塞来昔布能够有效抑制大鼠OIR模型视网膜新生血管的生长,且抑制效果与剂量正相关,其作用可能通过抑制VEGF表达实现。
[Abstract]:Objective to investigate the effect of celecoxib on retinal neovascularization in OIR by injecting celecoxib into vitreous cavity of Oxygen-induced retinopathy (OIR) model in rats. Methods Ninety-six 7-day-old SD neonatal rats were randomly divided into 6 groups: control group (n = 6): control group (n = 10), group A (n = 10), control group (n = 5), group B, n = 5 渭 g, group C, n = 20 渭 g, group D, n = 80 渭 g, respectively. OIR model was established in all groups except group Z in normal environment. The neonatal rats were given intravitreous injection of DMSO and celecoxib at the same dose on the 12th day after birth, and were killed on the 17th day after birth. The morphology of retinal tissue was observed by HE staining and the number of vascular endothelial nuclei breaking through the inner boundary membrane was counted. The expression of VEGF protein was detected by immunohistochemical staining in paraffin sections. Results HE staining showed that the number of vascular endothelial nuclei breaking through the membrane of retina was 0.44 卤0.18, 30.60 卤5.36), 19.58 卤4.58) and 7.58 卤2.68, respectively, in group A and B, with the exception of group O and group A, the number of vascular endothelial nuclei in group D was 7.58 卤2.68, and that in group B was 7.58 卤2.68, with the exception of group O and group A, which were 28.05 卤4.68 and 10.13 卤1.93, respectively. After celecoxib treatment, the number of vascular endothelial nuclei which broke through the inner boundary membrane decreased significantly and was positively correlated with the dose, the immunohistochemical results showed that the expression of VEGF protein was negative in group Z. The positive expression rate of VEGF protein in group A was higher than that in group C (P < 0.05). The positive rate of VEGF protein in group A was 900.The positive rate was higher than that in group B and C respectively, and the positive expression of VEGF protein in group C was decreased to 6842G and 30mg / kg in the latter three groups respectively, and the positive rate was higher than that in group C (P < 0.05), and the positive expression of VEGF protein in group A was significantly higher than that in group C (P < 0.05). Conclusion Celecoxib can effectively inhibit the growth of retinal neovascularization in rat OIR model, and the inhibitory effect is positively correlated with the dose, which may be achieved by inhibiting the expression of VEGF.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R774.1

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