异种脱钙骨基质与BMSCs混合后植入豚鼠鼓泡腔增强成骨效应的研究

发布时间：2018-04-30 00:34

本文选题：骨髓间充质干细胞 + 异种脱钙骨基质　；参考：《新疆医科大学》2012年硕士论文

【摘要】：目的：乳突填塞术对于重建开放式乳突手术后耳部的解剖结构与功能，改善病员的预后有明显作用。但在填塞材料的选择仍有较多困扰，，目前运用较多的自体组织和人工代用品均有其固有的缺点。本研究通过将异种脱钙骨基质和骨髓间充质干细胞混合后植入豚鼠鼓泡腔，观察其成骨效能，了解其用于乳突填塞的可行性及临床应用价值，为乳突填塞寻找新的材料来源。方法：取24只250～300g普通级健康豚鼠进行实验。取4只豚鼠的骨髓通过培养、分离、纯化以获得第三代骨髓间充质干细胞，用Brdu对第三代骨髓间充质干细胞进行标记。取牛髂骨以制备脱钙骨基质颗粒。将20只豚鼠随机分为两组，两组数量相等：1）实验组：牛髂骨制备的脱钙骨基质与骨髓间充质干细胞混合植入豚鼠鼓泡腔；2）对照组：取豚鼠自体髂骨植入豚鼠鼓泡腔。分别于手术后1个月、2个月进行CT扫描，用量表对其成骨量进行评分，比较两组间的成骨量，用秩和检验进行分析。2个月CT扫描后处死实验组及对照组豚鼠，取颞骨制作病理标本进行HE染色及免疫荧光检测。结果：实验结束时实验组有9只豚鼠、对照组有8只豚鼠，植入后2个月通过CT扫描观察实验组成骨量多于对照组，两组比较差异有统计学意义（P＜0.05）。病理证实实验组和对照组的植入物通进软骨形成的分化而产生骨组织。结论：实验组新骨形成成功修复了豚鼠鼓泡腔大小的骨缺损，异种脱钙骨基质与骨髓间充质干细胞混合后植入乳突腔能有效促进乳突填塞术后骨的形成。
[Abstract]:Objective: mastoid tamponade can reconstruct the anatomic structure and function of the ear after open mastoid surgery and improve the prognosis of the patients. However, there are still many problems in the selection of filling materials. At present, more autogenous tissues and artificial substitutes have their inherent disadvantages. In this study, xenogeneic decalcified bone stromal cells and bone marrow mesenchymal stem cells were mixed and implanted into the alveolar cavity of guinea pig to observe their osteogenic efficacy, to understand the feasibility and clinical application value of xenogeneic decalcified bone stroma and bone marrow mesenchymal stem cells in order to find a new material source for mastoid tamponade. Methods: 24 normal guinea pigs of 250 and 300 g were used in the experiment. The third generation of bone marrow mesenchymal stem cells were obtained from 4 guinea pigs by culture, isolation and purification. The third generation bone marrow mesenchymal stem cells were labeled with Brdu. Bovine iliac bone was taken to prepare decalcified bone matrix granules. Twenty guinea pigs were randomly divided into two groups. The two groups were divided into two groups: the experimental group: the decalcified bone stroma prepared by bovine iliac bone and bone marrow mesenchymal stem cells were mixed into the alveolar cavity of guinea pig. The control group: the autogenous iliac bone of guinea pig was implanted into the alveolar cavity of guinea pig. Ct scans were performed at 1 month and 2 months after operation respectively. The bone mass of the two groups was evaluated by the dosage table. The bone mass of the two groups was analyzed by rank sum test, and the guinea pigs of the experimental group and the control group were killed after 2 months CT scan, and the experimental group and the control group were killed after 2 months CT scan. Pathological specimens of temporal bone were made for HE staining and immunofluorescence detection. Results: at the end of the experiment, there were 9 guinea pigs in the experimental group and 8 guinea pigs in the control group. The bone mass of the experimental group was more than that of the control group by CT scan 2 months after implantation. The difference between the two groups was statistically significant (P < 0.05). It was confirmed by pathology that the implants of the experimental group and the control group had bone tissue due to the differentiation of cartilage. Conclusion: the new bone formation in the experimental group successfully repaired the bone defect in the alveolar cavity of guinea pig. The bone formation after implantation of xenogeneic decalcified bone stroma and bone marrow mesenchymal stem cells into mastoid cavity can effectively promote the formation of bone after mastoid tamponade.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R764

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