D-半乳糖致衰老豚鼠喉部病理改变研究

发布时间：2018-05-01 04:21

本文选题：D半乳糖 + 衰老　；参考：《武汉大学》2012年博士论文

【摘要】：目的：研究D-半乳糖致衰老豚鼠和自然老年豚鼠喉部细胞外基质成分和水通道蛋白AQP1-5的表达情况,探讨D-半乳糖致衰老豚鼠用于老年嗓音研究的可行性及水通道蛋白在豚鼠喉部老化过程中的作用。方法：选取耳廓反射灵敏、无噪声暴露史和耳毒性药物应用史的4月龄豚鼠32只,体重300～550g,随机分为对照组和D-半乳糖致衰老模型组,每组16只。另选相同条件自然衰老的2年龄豚鼠16只,体重600～900g,作为老年组。对照组和老年组动物不作任何处理,模型组动物颈背部皮下注射D-半乳糖300mg/kg.d (40mg/ml),每天一次,连续6周,致其衰老。然后各组动物断头处死,快速取出喉部,分别应用Alcian染色法、Masson三色染色法及免疫组织化学染色法观察三组豚鼠喉部透明质酸、胶原蛋白及水通道蛋白1～5的表达情况,并用Image-Pro Plus6.0For Window (IPP6.0)图像分析软件进行分析。结果：对照组、老年组、模型组透明质酸表达的灰度值分别为0.798351、0.694163和0.724869,三组豚鼠喉部透明质酸的含量依次为对照组模型组老年组；胶原蛋白表达的灰度值分别为0.632295、0.71147和0.768211,三组豚鼠喉部胶原蛋白的含量依次为对照组老年组模型组,三组间差异均有统计学意义(P0.05)。豚鼠喉部有AQP1～5表达,AQP1主要表达在喉粘膜假复层纤毛柱状上皮、固有层的腺体(包括浆液性和粘液性腺体)以及小血管内皮细胞,三组表达强度依次为对照组模型组老年组,老年组未见表达；AQP2主要表达于黏膜固有层的粘液性腺体和炎性浸润细胞,黏膜上皮细胞无或弱表达,AQP3高表达于黏膜假复层纤毛柱状上皮,腺体、血管内皮细胞和肌纤维组织均无表达,三组间AQP2和AQP3的表达无明显差异；AQP4主要表达于喉粘膜鳞状上皮、假复层柱状纤毛上皮、固有层粘膜腺体、肌纤维及部分浸润细胞,血管内皮细胞无表达,三组表达强度依次为对照组模型组老年组,其中老年组基本无表达或弱表达；AQP5主要表达于喉粘膜假复层纤毛柱状上皮细胞、上皮基底细胞、黏膜下浆液性腺体及导管,粘液性腺体无表达,三组表达强度依次为对照组老年组模型组。结论：与4月龄豚鼠相比较,D-半乳糖所致衰老豚鼠喉部透明质酸含量有一定程度的降低,胶原蛋白含量有一定程度的增加,与自然衰老豚鼠喉部变化一致,D-半乳糖所致衰老豚鼠可能可以用于老年嗓音的实验研究。豚鼠喉部有AQP1～5表达,老年豚鼠和D-半乳糖致衰老模型豚鼠喉部AQP1、AQP4的表达明显低于4月龄豚鼠,AQP5的表达明显高于4月龄豚鼠,AQP1、AQP4和AQP5可能与豚鼠喉部组织的老化改变有关。
[Abstract]:Objective : To study the expression of AQP1 - 5 in the extracellular matrix and AQP1 - 5 in guinea pig and naturally aged guinea pig , and to investigate the feasibility of D - galactose - induced aging guinea pig for the elderly voice study and the role of water channel protein in the aging of guinea pig ' s throat .

Methods : Thirty - four months old guinea pigs were randomly divided into control group and D - galactose - induced aging model group . The rats were randomly divided into two groups : control group and D - galactose - induced aging model group .

Results : The gray values of hyaluronic acid expression in the control group , the old group and the model group were 0.798351 , 0.694163 and 0.869 respectively , and the contents of hyaluronic acid in the laryngeal part of the three groups were in turn the control group model group and the old group ;
The gray values of the collagen expression were 0.632295 , 0.71147 and 0.768211 , respectively . The contents of collagen in the laryngeal part of the guinea pigs were statistically significant ( P0.05 ) . AQP1 was expressed in the laryngeal mucosa pseudostratified ciliated columnar epithelium , the lamina propria gland ( including serous fluid and mucinous gland ) and the small vascular endothelial cells .
AQP2 was mainly expressed in mucous glands of lamina propria and inflammatory infiltrate cells , and there was no or weak expression of AQP3 , AQP3 was expressed in the ciliated columnar epithelium , glandular , vascular endothelial cells and muscle fiber tissues of the mucosa pseudostratified ciliaris , and there was no significant difference between AQP2 and AQP3 in the three groups .
AQP4 was mainly expressed in laryngeal mucosa squamous epithelium , pseudostratified columnar ciliated epithelium , lamina propria muscularis propria , muscle fibers and partially infiltrated cells , and vascular endothelial cells were not expressed .
AQP5 was mainly expressed in recurrent laryngeal mucosa pseudostratified ciliated columnar epithelial cells , epithelial basement cells , submucous serous glands and catheters , and mucinous glands were not expressed .

Conclusion : Compared with 4 - month - old guinea pigs , the content of hyaluronic acid in the laryngeal part of guinea pig due to D - galactose is reduced to some extent , and the content of collagen is increased , and the aging guinea pig caused by D - galactose can be used in the experimental study of senile voice . AQP1 - 5 expression in the laryngeal part of guinea pig , AQP1 and AQP4 expression in guinea pig and D - galactose induced aging model are obviously lower than that of 4 - month - old guinea pigs , AQP1 , AQP4 and AQP5 may be related to the aging change of the laryngeal tissue of guinea pig .

【学位授予单位】：武汉大学
【学位级别】：博士
【学位授予年份】：2012
【分类号】：R-332

【参考文献】