眼睑鳞癌和基底细胞癌中激活蛋白AP-1、程序性细胞死亡因子PDCD4蛋白与p73蛋白表达的研究

发布时间：2018-05-02 01:55

本文选题：激活蛋白-1(AP-1) + 程序性细胞死亡因子-4(PDCD4)　；参考：《青岛大学》2012年硕士论文

【摘要】：目的观察AP-1(激活蛋白-1)、PDCD4(程序性细胞死亡因子-4)与p73蛋白在眼睑鳞癌不同病理分期和基底细胞癌的表达及临床意义,并探讨三者与眼睑基底细胞癌和鳞癌发生发展的关系。方法实验研究：用免疫组化染色方法检测68例眼睑鳞癌和40例基底细胞癌,并以20例正常眼睑组织作对照,检测三类组织中AP-1、 PDCD4与p73蛋白的表达,并比较三种蛋白在这两种癌组织和正常眼睑组织中的表达差异和在不同分期的眼睑鳞癌组织中的表达差异,X2检验比较各因子表达阳性率的差异。观察眼睑鳞癌和基底细胞癌中AP-1、 PDCD4与p73蛋白表达与性别、年龄、病理分期等的相互关系。结果 1AP-1在眼睑鳞癌和基底细胞癌中阳性表达率明显高于正常的眼睑组织,经统计学处理,差异有显著性(P0.05)。鳞癌和基底细胞癌中的AP-1阳性表达率无显著差异(P0.05)。AP-1在眼睑鳞癌和基底细胞癌患者中的表达与年龄、性别、有无淋巴转移无显著性差异；而在鳞癌不同的病理分期中的阳性表达率差异有统计学意义(P0.05)。 2PDCD4蛋白在眼睑正常组织中高表达,而在鳞癌和基底细胞癌中阳性表达率明显低于正常的眼睑组织,经统计学处理,差异有显著性(P0.05)。鳞癌和基底细胞癌中的PDCD4阳性表达率无显著差异(P0.05)。PDCD4在鳞癌和基底细胞癌患者中的表达与年龄、性别、有无淋巴转移无显著性差异；而在鳞癌不同的病理分期中的阳性表达率差异有统计学意义(P0.05)。 3p73在正常眼睑组织的基底细胞层表达,而随着细胞的角化,p73表达下降。鳞癌中p73表达轻度升高,与眼睑正常组织差异无统计学意义(P0.05),基底细胞癌中p73表达明显升高,阳性表达率有统计学差异(P0.05)。p73在鳞癌和基底癌患者中的表达与年龄、性别无显著性差异；而在鳞癌不同的病理分期中的阳性表达率差异具统计学意义(P0.05)。 4AP-1与PDCD4两种蛋白在鳞癌中的表达呈负相关,r=-0.401(P0.05),AP-1与p73,p73与PDCD4两组蛋白在鳞癌中的表达无相关性(P0.05)。结论 1AP-1蛋白的阳性表达率在鳞癌和基底癌组织中显著高于正常眼睑组织,AP-1蛋白在不同的鳞癌病理分期中的阳性表达率有显著性差异,提示AP-1与鳞癌的发生、发展密切相关。 2PDCD4在正常眼睑组织中表达率高于眼睑鳞癌和基底细胞癌,差异有统计学意义,提示PDCD4与鳞癌和基底细胞癌的发生发展可能成负相关,PDCD4可能在某种程度上抑制眼睑癌症的发生与发展。 3p73在正常眼睑组织的基底细胞层高表达,而随着细胞的角化,p73表达下降。鳞癌中p73表达轻度升高,差异无统计学意义；基底细胞癌中p73表达升高,差异具有统计学意义,提示p73可能与细胞的分化相关。 4AP-1与PDCD4在眼睑鳞癌中表达呈负相关,提示AP-1可能与PDCD4蛋白在参与眼睑鳞癌发生中存在某种相互关系。
[Abstract]:Objective To observe the expression and clinical significance of AP-1 (activation protein -1), PDCD4 (programmed cell death factor -4) and p73 protein in different pathological stages and basal cell carcinoma of eyelid squamous cell carcinoma, and to explore the relationship between the three and the occurrence and development of eyelid basal cell carcinoma and squamous cell carcinoma.
Methods: 68 cases of eyelid squamous cell carcinoma and 40 cases of basal cell carcinoma were detected by immunohistochemical staining. The expression of AP-1, PDCD4 and p73 in the three tissues was detected in 20 normal eyelid tissues, and the differences in the expression of three proteins in the two kinds of cancerous tissues and normal eyelid tissues and the eyelid scales in different stages were compared. The difference of expression in cancer tissues. X2 test compared the difference of the positive rates of various factors. The relationship between the expression of AP-1, PDCD4 and p73 protein in the eyelid squamous cell carcinoma and the basal cell carcinoma was observed with sex, age and pathological stage.
Result
The positive expression rate of 1AP-1 in eyelid squamous cell carcinoma and basal cell carcinoma was significantly higher than that of normal eyelid tissue. The difference was significant (P0.05). There was no significant difference in the positive rate of AP-1 in squamous and basal cell carcinoma (P0.05). The expression of.AP-1 in eyelid squamous cell carcinoma and basal cell carcinoma was associated with age, sex, and lymph node metastasis. There was no significant difference, but there was a significant difference in the positive expression rate in different pathological stages of squamous cell carcinoma (P0.05).
The expression of 2PDCD4 protein was highly expressed in normal eyelid tissue, while the positive expression rate in squamous and basal cell carcinoma was significantly lower than that in normal eyelid tissue. The difference was significant (P0.05). There was no significant difference in the positive rate of PDCD4 in squamous and basal cell carcinoma (P0.05) and the expression of.PDCD4 in squamous and basal cell carcinoma patients There was no significant difference in age, sex and lymph node metastasis, but there was a significant difference in the positive expression rate in different pathological stages of squamous cell carcinoma (P0.05).
The expression of 3p73 was expressed in the basal cell layer of normal eyelid tissue, and the expression of p73 decreased with the keratinization of the cells. The expression of p73 in the squamous cell carcinoma was slightly elevated, and there was no statistical difference between the normal tissue and the normal eyelid (P0.05). The expression of p73 in the basal cell carcinoma was significantly elevated, and the positive expression rate was statistically different (P0.05) the expression of.P73 in the squamous and basal carcinoma patients. There was no significant difference in age and sex, but the positive expression rate in different pathological stages of squamous cell carcinoma was statistically significant (P0.05).
There was a negative correlation between the expression of 4AP-1 and PDCD4 protein in squamous cell carcinoma, r=-0.401 (P0.05), and there was no correlation between AP-1 and p73, p73 and PDCD4 two histone expression in squamous cell carcinoma (P0.05). Two.
conclusion
The positive expression rate of 1AP-1 protein was significantly higher in the squamous and basal tissues than in the normal eyelid tissue. The positive expression rate of AP-1 protein in the pathological stages of different squamous cell carcinoma showed significant difference, suggesting that AP-1 was closely related to the development of squamous cell carcinoma.
The expression rate of 2PDCD4 in normal eyelid tissue is higher than that of eyelid squamous cell carcinoma and basal cell carcinoma. The difference is statistically significant, suggesting that PDCD4 may be negatively related to the development and development of squamous cell carcinoma and basal cell carcinoma. PDCD4 may inhibit the occurrence and development of eyelid cancer to some extent.
The expression of 3p73 was expressed in the basal cell layer of normal eyelid tissue, and the expression of p73 decreased with the keratinization of the cells. The expression of p73 in squamous cell carcinoma was slightly elevated, and the difference was not statistically significant. The difference of p73 expression in basal cell carcinoma was statistically significant, suggesting that p73 may be related to the differentiation of cells.
There is a negative correlation between 4AP-1 and PDCD4 expression in eyelid squamous cell carcinoma, suggesting that AP-1 may be related to the involvement of PDCD4 protein in the development of eyelid squamous cell carcinoma.

【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.71

【参考文献】