5-氮杂胞苷增加鼻咽癌细胞株C666-1放射敏感度的实验

发布时间：2018-05-03 14:10

本文选题：-氮杂胞苷 + 鼻咽癌细胞　；参考：《肿瘤防治研究》2017年02期

【摘要】：目的研究5-氮杂胞苷(5-azacytidine,5-Aza)对人鼻咽癌细胞C666-1放射敏感度的影响及可能机制。方法用不同浓度5-氮杂胞苷(0、500、1000、3000、5000 nmol/L)处理C666-1细胞,MTT法检测C666-1细胞增殖,流式细胞仪检测C666-1细胞周期,Western blot法检测细胞凋亡相关蛋白cleaved Caspase-3、cleaved PARP及DNA损伤相关蛋白γ-H2AX的表达。结果 5-氮杂胞苷随着药物浓度的增加,对C666-1细胞生长抑制作用增强。用1 000 nmol/L 5-氮杂胞苷处理鼻咽癌细胞进行放射增敏研究。鼻咽癌细胞C666-1接受单纯电离辐射时SF2(照射2 Gy时的细胞存活率)为48%,5-氮杂胞苷联合电离辐射时SF2降为31%,放射增敏比为1.37。5-氮杂胞苷能增加电离辐射诱导凋亡相关蛋白cleaved Caspase-3及cleaved PARP的表达。结论 5-氮杂胞苷增加鼻咽癌C666-1细胞对电离辐射的敏感度,可能的机制为5-氮杂胞苷能增加电离辐射诱导细胞凋亡。
[Abstract]:Objective to study the effect of 5-azacytidine on radiosensitivity of human nasopharyngeal carcinoma cell line C666-1 and its possible mechanism. Methods C666-1 cells were treated with different concentrations of 5-azacytidine (0500nmol / L) and C666-1 cells were treated with different concentrations of 5-azacytidine. The proliferation of C666-1 cells was detected by MTT assay. The expression of apoptosis-related protein cleaved Caspase-3 cleaved PARP and DNA damage associated protein 纬 -H2AX was detected by flow cytometry. Results with the increase of drug concentration, 5-azacytidine inhibited the growth of C666-1 cells. The radiosensitization of nasopharyngeal carcinoma cells treated with 1 000 nmol/L 5 azacytidine was studied. SF _ 2 (cell survival rate at 2 Gy irradiation) of nasopharyngeal carcinoma C666-1 cells receiving pure ionizing radiation was 48%. SF2 decreased to 31 when combined ionizing radiation with 5-azacytidine. The radiosensitization ratio of 1.37.5% azacytidine increased the apoptosis-related eggs induced by ionizing radiation. Expression of white cleaved Caspase-3 and cleaved PARP. Conclusion 5-azacytidine increases the sensitivity of nasopharyngeal carcinoma C666-1 cells to ionizing radiation, and the possible mechanism is that 5-azacytidine can increase the apoptosis induced by ionizing radiation.
【作者单位】：桂林医学院附属医院放疗科;
【基金】：广西自然科学基金(青年基金)(2013GXNS FBA019155) 广西区域性高发肿瘤早期防治研究重点实验室开放课题基金(GK2014-TKF04)
【分类号】：R739.63;R730.55

【参考文献】