miR-34a在鼻息肉上皮细胞中的表达及其对鼻黏膜上皮间质转化的影响

发布时间：2018-05-04 09:51

本文选题：鼻息肉 + 微小RNA　；参考：《临床耳鼻咽喉头颈外科杂志》2017年14期

【摘要】：目的:研究miR-34a在鼻息肉上皮细胞中的表达并探讨其对低氧诱导鼻黏膜上皮间质转化的影响。方法:利用实时荧光定量PCR检测鼻息肉和正常下鼻甲黏膜上皮细胞中miR-34a的表达情况;对正常鼻黏膜上皮细胞进行原代培养,并予低氧处理,通过Western-blot检测miR-34amimics转染对上皮间质转化相关蛋白(Ecadherin,α-SMA)表达的影响。结果:与正常下鼻甲相比,miR-34a在鼻息肉上皮细胞中表达减少,差异具有统计学意义(P0.05);低氧下调鼻黏膜上皮细胞中miR-34a表达,同时E-cadherin表达减少,α-SMA表达增加;这一效应能被miR-34amimics抑制。结论:miR-34a抑制低氧诱导的鼻黏膜上皮间质转化,可能在鼻息肉发生的病理生理过程中起重要作用。
[Abstract]:Aim: to investigate the expression of miR-34a in nasal polyp epithelial cells and its effect on hypoxia induced epithelial interstitial transformation of nasal mucosa. Methods: the expression of miR-34a in nasal polyps and normal inferior turbinate mucosal epithelial cells was detected by real-time fluorescence quantitative PCR, and the normal nasal mucosa epithelial cells were cultured in primary culture and treated with hypoxia. The effect of miR-34amimics transfection on the expression of E-cadherin (伪 -SMA) was detected by Western-blot. Results: compared with the normal inferior turbinate, the expression of miR-34a in nasal polyp epithelial cells decreased, the difference was statistically significant (P 0.05), and hypoxia decreased the expression of miR-34a, decreased the expression of E-cadherin and increased the expression of 伪 -SMA, which could be inhibited by miR-34amimics. Conclusion it is suggested that the inhibition of hypoxia induced epithelial mesenchymal transformation by 1% miR-34a may play an important role in the pathophysiological process of nasal polyps.
【作者单位】：海南省人民医院耳鼻咽喉头颈外科;
【基金】：国家自然科学基金(No:81200732) 广东省自然科学基金(No:S2012040006823) 广州市属高校“羊城学者”青年科研骨干培养项目(No:1201581626) 海南省重点研发计划项目(No:ZDYF2017115)
【分类号】：R765

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