雷帕霉素对缺血再灌注损伤大鼠视网膜组织结构、内层厚度的影响及其机制

发布时间：2018-05-05 21:09

本文选题：雷帕霉素 + 视网膜　；参考：《山东医药》2017年11期

【摘要】：目的观察雷帕霉素对缺血再灌注损伤(RIRI)大鼠视网膜组织结构、内层厚度的影响,并探讨其机制。方法将65只健康成年SD大鼠随机分为对照组5只、模型组30只和观察组30只。模型组和观察组采用前房灌注法制备RIRI大鼠模型,观察组大鼠造模前2 h腹腔注射雷帕霉素(2 mg/kg),模型组大鼠腹腔注射等量的生理盐水。对照组不作任何处理。造模后0、2、6、12、24、48 h模型组、治疗组分别处死5只大鼠,对照组5只大鼠实验结束后处死,摘除眼球制成石蜡切片。HE染色光镜下观察各组大鼠视网膜组织形态,测量视网膜内层厚度;用免疫组化法检测各组大鼠视网膜组织中的Caspase-7、Calpain-1表达的平均光密度(AOD)。结果模型组大鼠造模后早期视网膜水肿增厚,晚期各层细胞排列疏松,视网膜神经节细胞数目减少,发生空泡样变性。观察组大鼠视网膜损伤程度和视网膜神经节细胞空泡变性程度均低于模型组相同时点。造模后0、2 h观察组视网膜内层厚度小于模型组(P均0.05),造模后6、12、24、48 h观察组大鼠视网膜内层厚度大于模型组(P均0.05)。造模后各时点观察组视网膜组织Caspase-7、Calpain-1的AOD均低于模型组相同时点(P均0.05),但均高于对照组(P均0.05)。结论雷帕霉素可以减轻RIRI大鼠视网膜损伤程度,其机制可能与其降低视网膜组织Caspase-7、Calpain-1表达有关。
[Abstract]:Objective to observe the effects of rapamycin on retinal tissue structure and inner layer thickness in rats with ischemia-reperfusion injury (RIRI) and to explore its mechanism. Methods 65 healthy adult SD rats were randomly divided into control group (n = 5), model group (n = 30) and observation group (n = 30). The RIRI model was established in the model group and the observation group by anterior chamber perfusion. The rats in the observation group were intraperitoneally injected with rapamycin 2 mg / kg of rapamycin 2 mg 路kg ~ (-1) before the model was made, and the model group was injected with the same amount of normal saline. No treatment was given in the control group. 5 rats were killed in the treatment group and 5 rats in the control group were killed after the experiment. The eyeballs were removed and made into paraffin section. The morphology of retinal tissue was observed under light microscope and the thickness of the inner layer of retina was measured under light microscope. The average optical density of Caspase-7 calpain-1 expression in the retina of rats in each group was detected by immunohistochemical method. Results in the model group, the retinal edema thickened in the early stage, the cells in the late stages were loose, the number of retinal ganglion cells decreased, and vacuolar degeneration occurred. The degree of retinal injury and degeneration of retinal ganglion cells in the observation group were lower than those in the model group at the same time point. The thickness of the inner retinal layer in the observation group was lower than that in the model group (P < 0.05) 2 hours after the model making, and the thickness of the inner retinal layer in the observation group was greater than that in the model group (P < 0.05). The AOD of Caspase-7 calpain-1 in the retina of the observation group was lower than that of the model group at the same time points (P < 0.05), but higher than that of the control group (P = 0.05). Conclusion rapamycin can attenuate the degree of retinal injury in RIRI rats, and its mechanism may be related to the decrease of Caspase-7 calpain-1 expression in retinal tissues.
【作者单位】：广西医科大学第一附属医院;
【基金】：广西自然科学基金资助项目(2014GXNSFAA118273) 广西教育厅高校科研项目(YB2014072)
【分类号】：R774.1

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