Tregs及IL-6、IL-10、TNF在急性分泌性中耳炎患者中的变化及意义

发布时间：2018-05-11 01:35

本文选题：急性分泌性中耳炎 + CD+CD+调节性T细胞　；参考：《实用医学杂志》2016年02期

【摘要】：目的:检测CD4+CD25+调节性T细胞(Tregs)及IL-6、IL-10、TNF在急性分泌性中耳炎患者急性期外周血及耳积液中的变化。方法:采用BD CBA Flex Set IL-6、IL-10、TNF试剂盒,利用流式细胞术微球阵列法检测10例正常对照组外周血、37例(37耳)急性上呼吸道感染诱发急性分泌性中耳炎患者(患者组)外周血及耳积液中3种细胞因子水平及外周血Tregs细胞的比例。结果:患者组Tregs细胞的百分比明显高于正常对照组[(12.82±4.56)%vs.(6.695±1.09)%,P0.05]患者组外周血IL-6、IL-10、TNF浓度与正常对照组比较无统计学意义(P0.05),患者耳积液中3种细胞因子的浓度均明显高于患者及正常对照组外周血(P0.05)。结论:急性分泌性中耳炎患者Tregs细胞高表达,耳积液中3种细胞因子浓度明显升高。
[Abstract]:Objective: to detect the changes of CD4+CD25+ regulatory T cells (Tregs) and IL-6, IL-10, TNF in acute peripheral blood and auricular fluid in patients with acute secretory otitis media. Methods: BD CBA Flex Set IL-6, IL-10, and kit were used to detect the peripheral blood of 10 normal controls and 37 cases of acute upper respiratory tract infection (37 ears). The level of 3 cytokines in peripheral blood and auricular fluid in patients with acute secretory otitis media (patients group) and the proportion of Tregs cells in peripheral blood. Results: the percentage of Tregs cells in the patient group was significantly higher than that of the normal control group [(12.82 + 4.56)%vs. (6.695 + 1.09)%, IL-6, IL-10, and TNF in the P0.05] patients were no more than the normal control group. In P0.05, the concentration of 3 cytokines in the hydrops of the patients was significantly higher than that of the peripheral blood (P0.05) in the patients and the normal control group. Conclusion: the expression of Tregs cells in the patients with acute secretory otitis media is high, and the concentration of 3 cytokines in the hydrops is obviously increased.

【作者单位】：广东药学院临床医学院;广东省佛山市第一人民医院耳鼻咽喉头颈外科;广东省佛山市第一人民医院临床研究所;
【基金】：广东省科技计划项目(编号:2013B021800089)
【分类号】：R764.21

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