生长分化因子15与黏蛋白5AC在慢性鼻-鼻窦炎中的表达及意义

发布时间：2018-05-13 03:42

本文选题：鼻窦炎 + 黏蛋白AC　；参考：《临床耳鼻咽喉头颈外科杂志》2017年14期

【摘要】：目的:探讨生长分化因子15(GDF15)在慢性鼻-鼻窦炎(CRS)患者鼻黏膜中的表达及与黏蛋白5AC(MUC5AC)表达的关系。方法:选取15例伴鼻息肉的CRS患者,15例不伴鼻息肉的CRS患者和15例正常鼻腔黏膜,苏木精-伊红染色观察各组鼻黏膜的组织形态学改变,采用PAS染色检测各鼻黏膜中总黏蛋白的表达情况,免疫组织化学方法(IHC)、实时荧光定量PCR法(qRT-PCR)检测各组鼻黏膜中GDF15和MUC5AC的表达情况。结果:在伴或不伴鼻息肉的CRS患者鼻腔黏膜中可观察到黏膜增厚,杯状细胞、腺体增生以及炎性细胞浸润。IHC结果显示GDF15与MUC5AC主要分布于CRS鼻黏膜上皮及黏膜下的腺体内,较正常对照组明显增多,差异有统计学意义(P0.05)。qRT-PCR示GDF15和MUC5AC在CRS组织中的表达较正常组显著上升(P0.05),而在伴或不伴鼻息肉的CRS组织之间差异无统计学意义(P0.05)。伴或不伴鼻息肉的CRS中GDF15与MUC5AC的表达呈正相关(P0.05)。结论:GDF15和MUC5AC在伴或不伴鼻息肉的CRS患者中表达升高,且具有相关性,提示GDF15是CRS高分泌的一个重要因子。
[Abstract]:Objective: to investigate the expression of growth differentiation factor 15 (GDF 15) in nasal mucosa of patients with chronic rhinosinusitis (CRS) and its relationship with mucin 5ACMUC5AC. Methods: 15 cases of CRS with nasal polyps and 15 cases of CRS without nasal polyps and 15 cases of normal nasal mucosa were selected. The histomorphology of nasal mucosa in each group was observed by hematoxylin and eosin staining. The expression of total mucin in nasal mucosa was detected by PAS staining, and the expression of GDF15 and MUC5AC in nasal mucosa was detected by immunohistochemical method and real-time quantitative PCR method (qRT-PCR). Results: mucosal thickening, goblet cells, glandular hyperplasia and inflammatory cell infiltration were observed in nasal mucosa of CRS patients with or without nasal polyps. The results showed that GDF15 and MUC5AC were mainly distributed in the epithelial and submucous glands of CRS nasal mucosa. The expression of GDF15 and MUC5AC in CRS tissues was significantly higher than that in normal controls, but there was no significant difference between CRS tissues with or without nasal polyps. There was a positive correlation between the expression of GDF15 and MUC5AC in CRS with or without nasal polyps (P 0.05). Conclusion the expression of MUC5AC and GDF15 in CRS patients with or without nasal polyps was increased and correlated, suggesting that GDF15 is an important factor in the hypersecretion of CRS.
【作者单位】：南昌大学第一附属医院耳鼻咽喉头颈外科;江西省人民医院普外科;
【基金】：国家自然科学基金项目(No:81660170) 江西省卫生计生委科技计划项目(No:20165120) 江西省教育厅科学研究项目(No:GJJ150251)
【分类号】：R765

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