鼻咽清颗粒对鼻咽癌CNE-2细胞周期和凋亡的影响及可能机制

发布时间：2018-05-14 17:40

本文选题：鼻咽清颗粒 + 鼻咽癌细胞　；参考：《中国中西医结合耳鼻咽喉科杂志》2016年05期

【摘要】：目的探讨鼻咽清颗粒对鼻咽癌CNE-2细胞周期、凋亡影响及其可能作用机制。方法 CNE-2细胞Hoechst 33342-PI双染后,在荧光倒置显微镜下观察细胞形态,流式细胞仪检测(flow cytometry FCM)检测细胞周期和凋亡考察鼻咽清颗粒对CNE-2细胞周期和细胞凋亡的影响,West-blot考察鼻咽清颗粒对CNE-2细胞Caspase-3和Bcl-2蛋白表达的影响。结果与阴性对照组相比,随着鼻咽清颗粒浓缩液浓度增加,细胞凋亡率增加,G1的比例增加,West-blot实验结果表明,随着培养基中鼻咽清主药浓度的增加,促进细胞凋亡的Caspase-3蛋白表达增加,抑制细胞凋亡Bcl-2蛋白表达降低。结论鼻咽清颗粒能诱导caspase-3表达,下调Bcl-2蛋白表达,将CNE-2细胞阻滞于G1期,诱导细胞凋亡。
[Abstract]:Objective to investigate the effect of Biyanqing granule on the cell cycle and apoptosis of nasopharyngeal carcinoma (NPC) CNE-2 cells and its possible mechanism. Methods the morphology of CNE-2 cells was observed under fluorescence inverted microscope after Hoechst 33342-PI double staining. The effect of nasopharyngeal granule on the cell cycle and apoptosis of CNE-2 cells was investigated by flow cytometry FCM. The effect of BYG on the expression of Caspase-3 and Bcl-2 protein in CNE-2 cells was investigated by West-blot. Results compared with the negative control group, with the increase of the concentration of nasopharyngeal granule concentrate, the ratio of apoptosis rate increased and the proportion of G 1 increased. The results of West-blot experiment showed that with the increase of the concentration of the main drug in the medium, the concentration of the main drug was increased. The expression of Caspase-3 protein increased and the expression of Bcl-2 protein decreased. Conclusion BYQG can induce caspase-3 expression, down-regulate Bcl-2 protein expression, block CNE-2 cells in G1 phase and induce apoptosis.
【作者单位】：汕头大学医学院附属粤北人民医院;
【基金】：广东省中医药局项目(20122043) 韶关市医药卫生科研计划项目Y12159
【分类号】：R739.63

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