糖尿病大鼠视网膜组织醛脱氢酶活性与表达量变化及其意义

发布时间：2018-05-14 23:05

本文选题：糖尿病视网膜病变 + 醛脱氢酶　；参考：《山东医药》2017年08期

【摘要】：目的探讨醛脱氢酶(ALDH)在糖尿病视网膜病变发生、发展中的作用。方法选择SD大鼠40只,随机分为模型组、对照组各20只。模型组采用腹腔注射链脲佐菌素建立糖尿病模型,对照组同期腹腔注射等体积柠檬酸钠缓冲液。两组分别于建模3天及建模8、16周检测体质量及血糖;建模8、16周各处死10只,检测视网膜组织ALDH活性和表达量,采用免疫组化法检测视网膜组织bcl-2、bax表达,采用TUNEL法检测视网膜神经细胞凋亡指数。结果两组体质量随建模时间延长均明显增加(P均0.05),但血糖变化不明显;与对照组比较,模型组建模8、16周体质量明显低于对照组同期(P均0.05),而血糖水平明显高于对照组同期(P均0.05)。与对照组比较,模型组建模8、16周ALDH活性和表达量均明显升高(P均0.05);模型组建模16周ALDH活性和表达量均低于建模8周(P均0.05);对照组建模8、16周ALDH活性和表达量变化不明显(P均0.05)。模型组建模8、16周bcl-2、bax表达及视网膜神经细胞凋亡率均高于对照组同期,且建模16周明显高于建模8周(P均0.05),而对照组变化不明显(P0.05)。结论糖尿病大鼠视网膜组织ALDH活性与表达量升高,其活性与表达量变化可促进糖尿病大鼠视网膜神经细胞凋亡,且与凋亡严重程度有关。
[Abstract]:Objective to investigate the role of aldehydes dehydrogenase (ALDH) in the pathogenesis and development of diabetic retinopathy. Methods Forty SD rats were randomly divided into two groups: the model group (n = 20) and the control group (n = 20). The diabetic model was established by intraperitoneal injection of streptozotocin, while the control group was intraperitoneally injected with sodium citrate buffer. The body mass and blood glucose were detected in the two groups after 3 days and 816 weeks respectively, and 10 rats were killed in each group at 816 weeks. The activity and expression of ALDH in retinal tissues were detected, and the expression of bcl-2mbax in retinal tissues was detected by immunohistochemical method, and the expression of bcl-2mbax in retina was detected by immunohistochemical method. Apoptosis index of retinal nerve cells was measured by TUNEL method. Results compared with the control group, the body mass of the model group at 816 weeks was significantly lower than that of the control group, and the blood glucose level was significantly higher than that of the control group. Compared with the control group, the activity and expression of ALDH in the model group increased significantly at 816 weeks, the activity and expression of ALDH in the model group at the 16th week were lower than those in the model group at the 8th week (P 0.05), and the ALDH activity and expression in the control group were not significantly changed at 816 weeks after modeling (P < 0.05). The expression of bcl-2mbax and the rate of apoptosis of retinal nerve cells in the model group were significantly higher than those in the control group at 8 and 16 weeks, and were significantly higher than those in the model group at the 8th week and the control group respectively, but the changes in the control group were not obvious. Conclusion the activity and expression of ALDH in the retina of diabetic rats were increased, and the changes of the activity and expression of ALDH could promote the apoptosis of retinal nerve cells in diabetic rats, which was related to the severity of apoptosis.
【作者单位】：安徽医科大学第一附属医院;淮北市人民医院;
【分类号】：R587.2;R774.1

【相似文献】