EZH2对人喉鳞状细胞癌HEP-2增殖、迁移和侵袭的影响

发布时间：2018-05-15 01:32

本文选题：喉鳞状细胞癌 + HEP-　；参考：《南京医科大学学报(自然科学版)》2017年07期

【摘要】：目的:探讨Zeste基因增强子同源物2(EZH2)在人喉鳞状细胞癌组织和HEP-2、SCC10A细胞中的表达及其对人喉鳞状细胞癌细胞HEP-2增殖、迁移和侵袭的影响。方法:收集宣城中心医院30例喉鳞状细胞癌和10例癌旁正常喉黏膜组织标本,q RT-PCR检测喉鳞状细胞癌组织和癌旁组织以及喉鳞状细胞癌细胞HEP-2、SCC10A中EZH2的表达。采用Lipofectamine2000将EZH2 shRNA质粒转染至HEP-2细胞中,通过q RT-PCR和Western blot检测EZH2 shRNA的转染效率,并通过Western blot检测EZH2下游相关信号分子的表达水平。CCK8实验检测HEP-2细胞的增殖,划痕实验和Transwell侵袭实验检测细胞的迁移、侵袭能力。运用裸鼠体内成瘤实验检测EZH2对HEP-2细胞体内成瘤能力的影响。结果:EZH2在喉鳞状细胞癌组织及细胞中显著高表达(P0.01)。EZH2 shRNA质粒转染后可抑制细胞的增殖能力,细胞的迁移和侵袭也均受到明显抑制。裸鼠体内成瘤实验结果显示,EZH2 shRNA组的HEP-2细胞肿瘤形成能力明显减弱。结论:EZH2在喉鳞状细胞癌组织和细胞中高表达;EZH2 shRNA能够在体外抑制喉鳞状细胞癌细胞HEP-2的增殖、迁移和侵袭,并抑制RAF1-ERK信号通路的活化;EZH2能够在体内抑制喉鳞状细胞癌细胞HEP-2的成瘤能力。
[Abstract]:Aim: to investigate the expression of Zeste enhancer congener 2EZH2 in human laryngeal squamous cell carcinoma (LSCC) and its effect on HEP-2 proliferation, migration and invasion of human laryngeal squamous cell carcinoma (HSCC10A). Methods: the expression of EZH2 in laryngeal squamous cell carcinoma (LSCC) and adjacent laryngeal squamous cell carcinoma (LSCC) was detected by Q RT-PCR in 30 cases of laryngeal squamous cell carcinoma (LSCC) and 10 cases of adjacent normal laryngeal mucosa in Xuancheng Central Hospital. EZH2 shRNA plasmid was transfected into HEP-2 cells by Lipofectamine2000. The transfection efficiency of EZH2 shRNA was detected by Q RT-PCR and Western blot, and the expression level of signal molecules downstream of EZH2 was detected by Western blot. CCK8 was used to detect the proliferation of HEP-2 cells. The migration and invasiveness of cells were detected by scratch test and Transwell invasion assay. The effect of EZH2 on the tumorigenic ability of HEP-2 cells in vivo was detected by tumorigenesis test in nude mice. Results the cell proliferation was inhibited and the migration and invasion of the cells were also inhibited by the transfection of the shRNA plasmid p0.01. EZH2 in laryngeal squamous cell carcinoma tissues and cells. The results of tumorigenesis in nude mice showed that the tumor formation ability of HEP-2 cells in EZH2 shRNA group was significantly decreased. Conclusion the high expression of EZH2 shRNA in laryngeal squamous cell carcinoma can inhibit the proliferation, migration and invasion of HEP-2 in vitro. Inhibiting the activation of RAF1-ERK signaling pathway, EZH2 could inhibit the tumorigenesis of laryngeal squamous cell carcinoma HEP-2 in vivo.
【作者单位】：宣城市中心医院耳鼻咽喉科;
【分类号】：R739.65

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