褪黑素通过促凋亡协同增强顺铂对人喉癌细胞增殖的抑制作用

发布时间：2018-05-16 02:15

本文选题：褪黑素 + 顺铂　；参考：《中国肿瘤生物治疗杂志》2017年06期

【摘要】：目的:研究褪黑素(melatonin,MT)对人喉癌细胞增殖与凋亡的影响及MT增强人喉癌细胞对顺铂(cisplatin,DDP)治疗的敏感性。方法:采用不同质量浓度MT和DDP单独或联合处理Hep-2细胞;通过CCK-8法检测细胞增殖,流式细胞术检测细胞凋亡和细胞周期,采用两药相互作用指数(co-efficient of drug interaction,CDI)评估MT是否影响Hep-2细胞对DDP的敏感性。结果:CCK-8检测结果显示,单用MT或DDP可浓度依赖性抑制Hep-2细胞的增殖,MT可协同增强DDP对Hep-2细胞的增殖抑制作用(CDI1)。流式细胞术检测细胞凋亡和细胞周期结果显示,MT可促进Hep-2细胞凋亡以及增加亚G1期细胞比例(P0.01),MT可协同DDP促进Hep-2细胞凋亡[0.5 mmol/L MT联合20μg/ml DDP组的细胞凋亡率显著高于20μg/ml DDP组,(40.9±3.0)%vs(11.0±0.9)%,P0.01]以及亚G1期细胞比例[0.5 mmol/L MT联合20μg/ml DDP组的亚G1期细胞比例显著高于20μg/ml DDP组,(73.0±2.4)%vs(40.4±3.0)%,P0.01]。加入Caspase抑制剂Z-VAD-fmk可逆转MT和/或DDP对Hep-2细胞的增殖抑制作用和凋亡诱导作用(均P0.01)。结论:MT能以Caspase依赖的方式诱导人喉癌细胞Hep-2的凋亡,从而协同增强DDP对细胞的增殖抑制作用。
[Abstract]:Objective: To study the effect of melatonin (MT) on the proliferation and apoptosis of human larynx cancer cells and the sensitivity of MT enhanced human larynx cancer cells to cisplatin (cisplatin, DDP). Methods: Hep-2 cells were treated by MT and DDP with different mass concentrations, and the cell proliferation was detected by CCK-8, and cell apoptosis and cell cycle were detected by flow cytometry. Co-efficient of drug interaction (CDI) was used to evaluate the effect of MT on the sensitivity of DDP to Hep-2 cells. Results: CCK-8 detection results showed that the proliferation of Hep-2 cells was inhibited only by MT or DDP. Apoptosis and cell cycle results showed that MT could promote apoptosis of Hep-2 cells and increase the proportion of subG1 cells (P0.01). MT could promote apoptosis of Hep-2 cells with DDP, and the apoptosis rate of [0.5 mmol/L MT combined with 20 mu g/ml DDP group was significantly higher than that of 20 mu group, (40.9 + 3) (11 + 0.9)%. The percentage of subG1 cells in the 20 g/ml DDP group was significantly higher than that in the 20 g/ml DDP group, (73 + 2.4)%vs (40.4 + 3)%. P0.01]. added to the Caspase inhibitor Z-VAD-fmk could reverse the proliferation inhibition and apoptosis induction of MT and / or DDP on Hep-2 cells. The synergistic effect of DDP on the proliferation of cells was enhanced.

【作者单位】：解放军第455医院耳鼻咽喉头颈外科;第二军医大学长海医院病理科;
【基金】：国家自然科学基金资助项目(No.81502466) 南京军区医药卫生科研基金资助项目(No.14MS023)~~
【分类号】：R739.65

【相似文献】