Nd:YAG激光诱导棕色挪威大鼠脉络膜新生血管模型的建立与评价

发布时间：2018-05-16 15:32

本文选题：脉络膜新生血管 + Nd　；参考：《眼科新进展》2016年04期

【摘要】：目的评价采用不同功率的Nd:YAG激光(波长532 nm)诱导棕色挪威(Brown Norway,BN)大鼠脉络膜新生血管(choroidal neovascularization,CNV)模型,为相关的药物和器械评价提供适用的疾病动物模型。方法将96只BN大鼠随机分为3个实验组(分别为高、中、低功率组,激光功率依次为300 mW、200 mW、100 mW,光斑直径100μm,曝光时间100 ms,每眼光凝8个点)和1个空白对照组,每组24只。各实验组大鼠激光造模后的第1周、2周、3周、4周行眼底荧光血管造影(fundus fluorescein angiography,FFA),每个时间点各组随机抽取2只大鼠行脉络膜铺片,随机抽取4只大鼠双眼行HE染色,以对比各组间CNV荧光素渗漏率、面积、厚度等差异。结果眼底彩照及FFA结果:中、高功率组第1周、2周、3周眼底荧光素渗漏率均高于低功率组(均为P0.01),中、高功率组差异均无统计学意义(均为P0.05),空白对照组晚期FFA图像未见荧光素渗漏。脉络膜铺片结果:低功率组第1周、2周CNV面积变化不明显,中、高功率组CNV面积从1周起随时间增加而增大,各组CNV面积至第3周达到高峰,高功率组第4周的CNV面积仍大于低功率组(P0.05)。HE染色结果:各实验组光凝后第1周,视网膜内核层排列紊乱,大量增生的细胞穿破RPE层向视网膜内层移行,形成初期CNV,随时间推移CNV逐渐增厚,区域内可见色素巨噬细胞、成纤维细胞,并夹杂新生血管,第4周区域周围可见大量胶原纤维,各组CNV中央厚度变化与面积变化一致,均在第3周达到高峰,且第4周高功率组CNV中央厚度大于低功率组(P0.05),对照组视网膜内核层细胞排列整齐,未见炎性反应。结论 100 mW、200mW、300 m W的Nd:YAG激光均可以诱导BN大鼠CNV模型,其中300 m W的成模率最高,CNV面积及中央厚度最大,是Nd:YAG激光诱导BN大鼠CNV模型较为理想的激光功率。
[Abstract]:Objective to evaluate the choroidal neovascularization model induced by different power Nd:YAG laser (wavelength 532nm) in Brown NorwayBN rats, and to provide a suitable animal model for the evaluation of related drugs and instruments. Methods 96 BN rats were randomly divided into three experimental groups (high, middle and low power groups), with laser power of 300 MW / 200mW / W, light spot diameter 100 渭 m, exposure time 100msper eye, and a blank control group (24 rats in each group). Three weeks and four weeks after laser modeling, fundus fluorescein angiographic FFAA was performed in each experimental group. Two rats were randomly selected for choroidal preparation at each time point, and 4 rats were randomly selected for HE staining. The leakage rate, area and thickness of CNV fluorescein were compared. Results the fundus color radiography and FFA results showed that the fundus fluorescein leakage rate in the high power group was higher than that in the low power group (P 0.01, P 0.01, P < 0.05), and the fundus fluorescein leakage rate in the high power group was higher than that in the low power group. There was no significant difference between the high power group and the control group (P 0.05). No fluorescein leakage was found in the late FFA images of the blank control group. The results of choroidal film preparation showed that the area of CNV in the low power group did not change significantly at the 1st week and 2nd week. The area of CNV in the high power group increased with time from 1 week to the third week, and the area of CNV in each group reached its peak at the third week. The area of CNV in the high power group was still larger than that in the low power group at the 4th week. The results of HE staining were as follows: at the first week after photocoagulation, the nuclear layer of the retina was disarranged and a large number of proliferative cells broke through the RPE layer and migrated to the inner layer of the retina. In the early stage of CNV formation, CNV was gradually thickened with time. Pigment macrophages, fibroblasts and neovascularization were found in the region. A large number of collagen fibers were observed around the CNV area at the 4th week. The changes of central thickness of CNV were consistent with the area changes in each group. The central thickness of CNV in the high power group was larger than that in the low power group (P 0.05) at the 4th week, and the cells in the nuclear layer of the retina in the control group were arranged neatly and no inflammatory reaction was found. Conclusion the CNV model of BN rats can be induced by 100mW / 200mW / 300MW Nd:YAG laser, among which the mode-forming rate of 300MW is the highest and the central thickness is the largest, which is the ideal laser power for the CNV model of BN rats induced by Nd:YAG laser.
【作者单位】：四川大学华西公共卫生学院;四川大学华西医院国家成都新药安全性评价中心;
【基金】：科技部国家重大科学仪器设备开发专项(编号:2013YQ490859)~~
【分类号】：R-332;R773.4

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