慢病毒表达系统介导的RNAi技术靶向沉默Bi-1基因对鼻咽癌细胞生物学特性的影响

发布时间：2018-05-17 17:13

本文选题：鼻咽癌 + Bi-　；参考：《中国老年学杂志》2017年03期

【摘要】：目的探讨Bi-1基因的生物学功能及其与鼻咽癌发生与发展的相关性。方法首先构建以Bi-1为靶基因的RNA干扰(RNAi)质粒载体,采用慢病毒表达系统将其导入细胞,利用RNAi技术在细胞内诱导特异序列的基因沉默以观察对鼻咽癌细胞生长增殖的影响。结果靶向Bi-1沉默的慢病毒感染鼻咽癌细胞后能有效抑制细胞的生长增殖并诱导其凋亡,在荧光显微镜下可见经靶向Bi-1沉默的慢病毒处理后的鼻咽癌细胞呈现出细胞皱缩、核固缩、核断裂等典型的凋亡细胞形态,而且DNA凝胶电泳图谱呈现"梯状"现象;此外,Bi-1基因的沉默可有效地抑制裸鼠鼻咽癌移植瘤的生长。结论靶向沉默鼻咽癌细胞中的Bi-1表达,可抑制细胞生长增殖,并诱导细胞发生凋亡。
[Abstract]:Objective to investigate the biological function of Bi-1 gene and its relationship with the occurrence and development of nasopharyngeal carcinoma. Methods the RNA interference RNAi plasmid vector targeting Bi-1 gene was constructed and introduced into the cells by using lentivirus expression system. The specific sequence gene silencing was induced by RNAi technique to observe the effect on the growth and proliferation of nasopharyngeal carcinoma cells. Results Lentivirus silenced by Bi-1 could effectively inhibit the growth and proliferation of nasopharyngeal carcinoma cells and induce apoptosis. Under fluorescence microscope, the cells of nasopharyngeal carcinoma treated with targeted Bi-1 silencing lentivirus showed cell shrinkage. The typical apoptotic cell morphology such as nuclear pyknosis, nuclear breakage and DNA gel electrophoresis showed "ladder" phenomenon. In addition, the silencing of Bi-1 gene could effectively inhibit the growth of transplanted tumor of nasopharyngeal carcinoma in nude mice. Conclusion silencing the expression of Bi-1 in nasopharyngeal carcinoma cells can inhibit the growth and proliferation of nasopharyngeal carcinoma cells and induce apoptosis.
【作者单位】：淮安市第一人民医院耳鼻咽喉科;淮安市第一人民医院放疗科;
【分类号】：R739.63

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