MiR-124对鼻咽癌细胞株上皮间充质转化和放疗敏感性的影响

发布时间：2018-05-20 08:04

本文选题：MiR- + 鼻咽癌　；参考：《实用医学杂志》2016年17期

【摘要】：目的:研究miR-124对鼻咽癌细胞株上皮间充质转化(epithelial-mesenchymal transitions,EMT)和放疗敏感性的影响。方法:转染miR-124 mimic或inhibitor入鼻咽癌细胞株,划痕实验检测miR-124对鼻咽癌EMT影响;细胞转染成功24 h后X线照射,检测细胞株中Caspase-3活性及凋亡细胞比例;Ed U方法检测放疗后细胞增殖能力;Western blot检测信号通路蛋白。结果:划痕实验结果显示miR-124能抑制鼻咽癌细胞株EMT。Caspase-3活性检测结果提示,miR-124过表达细胞株接受放疗后Caspase-3活性显著上升(P0.01)。流式细胞仪结果显示,miR-124能增加细胞株放疗后凋亡。Ed U结果显示,miR-124能抑制放疗后细胞的增殖。Western blot结果表明过表达miR-124的细胞株接受放疗后,p-Akt含量显著下降。结论:miR-124可能通过Akt信号通路抑制鼻咽癌细胞株EMT,增加其放疗敏感性。这为鼻咽放疗增敏提供了新的可行的研究方向。
[Abstract]:Aim: to study the effects of miR-124 on epithelial-mesenchymal transitionsof nasopharyngeal carcinoma (NPC) cell line. Methods: after transfection of miR-124 mimic or inhibitor into nasopharyngeal carcinoma cell line, the effect of miR-124 on EMT of nasopharyngeal carcinoma was detected by scratch test. The activity of Caspase-3 and the proportion of apoptotic cells in the cell line were measured. The proliferative ability of the cells after radiotherapy and the signal pathway protein were detected by Western blot. Results: the results of scratch test showed that miR-124 could inhibit the EMT.Caspase-3 activity of nasopharyngeal carcinoma cell line. The results showed that the Caspase-3 activity of the overexpression cell line was significantly increased after radiotherapy (P 0.01). Flow cytometry showed that miR-124 could increase cell apoptosis after radiotherapy. Ed U showed that miR-124 could inhibit cell proliferation after radiotherapy. Western blot analysis showed that the content of p-Akt was significantly decreased in the cell line with overexpression of miR-124 after radiotherapy. Conclusion the Akt signaling pathway may inhibit the nasopharyngeal carcinoma cell line EMTs and increase its radiosensitivity. This provides a new and feasible research direction for nasopharyngeal radiotherapy.
【作者单位】：广州医科大学附属肿瘤医院放疗科一区;
【基金】：国家自然科学基金资助项目(编号:81502342) 广州医科大学博士启动基金资助项目(编号:2014C45)
【分类号】：R739.63

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