葡萄籽原花青素对大鼠硒性白内障的抑制作用及其机理研究

发布时间：2018-05-20 10:46

  本文选题：葡萄籽原花青素 + 亚硒酸钠性白内障　； 参考：《华中科技大学》2012年博士论文

【摘要】：目的:探讨不同剂量葡萄籽原花青素对大鼠硒性白内障的抑制作用 方法:将80只8日龄的SD大鼠随机分为对照组模型组和GSPE低中高剂量组对照组给予生理盐水皮下注射，模型组于第10日龄起给予亚硒酸钠20μmol/kg颈背部皮下注射，隔日1次，连续3次GSPE各组除给予同样造模外，从第8日龄起还分别给予低剂量组50mg/kg中剂量组100mg/kg高剂量组200mg/kg的GSPE灌胃，每天1次连续14天待乳鼠睁眼后用裂隙灯显微镜观察晶状体的浑浊程度，分级以及拍照，并每天测量晶状体核性浑浊斑块的最大直径变化光镜下HE染色观察各组晶状体组织的细胞形态 结果:模型组全部形成典型的核性白内障，造模成功率100%与模型组相比，GSPE各组的晶状体浑浊程度和核性浑浊斑块的最大直径明显减小（P0.05或P0.01或P0.001）光镜下HE染色发现GSPE干预后，晶状体组织的损伤程度减轻，且与GSPE有剂量关系 结论:该实验证实了GSPE能明显抑制亚硒酸钠引起的核性白内障的形成和发展，且有时间 剂量关系，其最低有效剂量为50mg/kg 目的:探讨葡萄籽原花青素提取物抑制大鼠硒性白内障的机理研究 方法:将80只8日龄的SD大鼠随机分为对照组模型组GSPE低中高剂量组对照组给予生理盐水皮下注射，模型组于第10日龄起给予亚硒酸钠20μmol/kg颈背部皮下注射，隔日1次，连续3次GSPE各组除给予同样造模外，从第8日龄起还分别给予低剂量组50mg/kg中剂量组100mg/kg高剂量组200mg/kg的GSPE灌胃，每天1次连续14天实验结束后测量各组晶状体中丙二醛(MDA)超氧化物歧化酶（SOD）过氧化氢酶（CAT）谷胱甘肽过氧化物酶（GSH-PX）一氧化氮（NO）钙离子（Ca~(2+)）含量及晶状体组织抑制羟自由基(OH-)的能力用免疫组织化学法观察iNOS calpainⅡ蛋白在各组晶状体上皮细胞中（LECs）的表达情况，并用平均吸光度(AOD)反应各组的表达量用实时荧光定量RT-PCR法检测iNOS calpainⅡmRNA在各组晶状体中的表达情况 结果:与对照组比，模型组晶状体中抗氧化酶（SOD CAT GSH-PX）的活性及抑制羟自由基的能力显著下降，同时MDA Ca~(2+) NO的含量和iNOS calpainⅡ蛋白吸光度及mRNA的表达水平显著上升（P0.01或P0.001）；与模型组相比，，GSPE各组抗氧化酶（SOD CAT GSH-PX）的活性及抑制羟自由基的能力明显升高，同时MDA Ca~(2+) NO的含量和iNOS calpainⅡ蛋白及mRNA的表达水平明显下降（P0.05或P0.01或P0.001），且与GSPE有剂量依赖性 结论:该实验证实了GSPE能显著抑制亚硒酸钠引起的核性白内障的形成，其作用机制可能与增强抗氧化酶（SOD CAT GSH-PX）的活性，阻止脂质过氧化产物MDA及自由基羟基的产生，以及抑制晶状体中iNOS和calpainⅡ的激活有关
[Abstract]:Objective: to investigate the inhibitory effect of different doses of grape seed procyanidins on selenium induced cataract in rats. Methods: eighty 8-day-old SD rats were randomly divided into two groups: the control group and the low, medium and high dose group of GSPE. The model group was subcutaneously injected with sodium selenite 20 渭 mol/kg, once every other day, and the model group was given subcutaneous injection of sodium selenite 20 渭 mol/kg every other day. In addition to the same model, each GSPE group was given intragastric administration of low dose 50mg/kg medium dose 100mg/kg high dose 200mg/kg GSPE from the 8th day of age. Once a day for 14 consecutive days, the degree of opacity, grading and taking pictures of the lens were observed by slit lamp microscope after the newborn rats opened their eyes. The maximum diameter change of lens nuclear opacification plaque was measured every day. The morphology of lens tissue in each group was observed by HE staining under light microscope. Results: typical nuclear cataract was formed in all of the model group. Compared with the model group, the degree of lens opacification and the maximum diameter of the nuclear opacifying plaque in the model group were significantly reduced by 100% success rate (P 0.05 or P 0.01 or P 0.001). The results of HE staining under light microscope showed that the degree of lens opacification and the maximum diameter of nuclear opacity plaque in the model group were significantly decreased compared with the model group. The degree of lens injury was reduced and there was a dose-dependent relationship with GSPE. Conclusion: GSPE can inhibit the formation and development of nuclear cataract induced by sodium selenite in a dose-dependent manner, and the lowest effective dose is 50mg/kg. Objective: to investigate the mechanism of grape seed proanthocyanidin extract (GPC) inhibiting rat selenium-induced cataract. Methods: eighty 8-day-old SD rats were randomly divided into two groups: the control group, the low, medium and high dose group of GSPE, the control group were subcutaneously injected with normal saline, and the model group was given subcutaneous injection of sodium selenite 20 渭 mol/kg into the neck and back from the 10th day, once every other day. In addition to the same model, each GSPE group was given intragastric administration of low dose 50mg/kg medium dose 100mg/kg high dose 200mg/kg GSPE from the 8th day of age. The content of malondialdehyde (MDAs) superoxide dismutase (SOD) and catalase (CAT) glutathione peroxidase (GSH-PX) (no) calcium in the lens of each group were measured once a day for 14 days. The expression of iNOS calpain 鈪

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