COX-2和VEGF在鼻咽癌中的表达及其临床意义

发布时间：2018-05-21 10:35

本文选题：鼻咽癌(NPC) + 环氧合酶-2(COX-2)　；参考：《郑州大学》2017年硕士论文

【摘要】：目的通过检测COX-2(环氧合酶-2)蛋白和VEGF(血管内皮生长因子)蛋白在人类鼻咽癌(nasopharyngeal carcinoma,NPC)组织及鼻咽部正常黏膜组织中的表达水平,分析两种蛋白的表达与NPC的关系,两种蛋白在NPC发生和演变中的相互关系及其临床意义。材料与方法标本来自2015年2月-2016年12月期间,在郑州大学第一附属医院鼻科行鼻内镜下鼻咽部新生物活检术的患者,所选患者入院前均未接受过放化疗以及非甾体类药物的治疗,且各项病例资料均完整。每位患者均取两部位组织,一是鼻咽部新生物部,二是其鼻咽部表面光滑未突出、肉眼观类正常部位。两部位组织经我院病理科分别确诊为鼻咽癌、鼻咽部正常黏膜组织,满足该条件的患者共42例,以鼻咽部新生物活检标本做为实验组,以其鼻咽部正常黏膜组织作为对照组,每位鼻咽癌患者活检标本和与之配对的对照组组织在活检后均被迅速放在-80℃冰箱内保存,全部患者都在知情同意书上确认签字。本文涉及的实验方法有组织总蛋白的提取、BCA法定量蛋白以及蛋白质印迹法(Western Blot法),通过以上方法检测COX-2蛋白和VEGF蛋白在实验组以及对照组的表达情况。使用SPSS21.0对本文所有数据进行统计学分析。Western Blot法获得的数据需经正态性检验,方差齐性分析,呈正态分布的资料用均数±标准差表示,配对样本t检验,独立样本t检验,Pearson相关性分析,把P0.05作为判断差异有统计学意义的标准。结果1.鼻咽癌组织中COX-2蛋白相对表达强度(0.824±0.147)较鼻咽部正常黏膜组织(0.201±0.082)明显增强(t=8.301,P=0.000)。2.鼻咽癌组织中VEGF蛋白相对表达强度(0.709±0.103)较鼻咽部正常黏膜组织(0.368±0.098)明显增强(t=5.343,P=0.001)。3.鼻咽癌组织中COX-2蛋白在有颈部淋巴结转移组中的相对表达强度(0.878±0.069)较无颈部淋巴结转移组(0.751±0.052)明显增强(t=3.310,P=0.011),COX-2蛋白在TNM分期为Ⅲ-Ⅳ期的相对表达强度(0.882±0.064)较Ⅰ-Ⅱ期(0.747±0.046)明显增强(t=3.844,P=0.005)。在不同性别、不同年龄组中的表达强度无显著差异(均为P0.05)。4.鼻咽癌组织中VEGF蛋白在有颈部淋巴结转移组中的相对表达强度(0.748±0.056)较无颈部淋巴结转移组(0.656±0.036)明显增强(t=2.604,P=0.031),VEGF蛋白在TNM分期为Ⅲ-Ⅳ期的相对表达强度(0.758±0.038)较Ⅰ-Ⅱ期(0.658±0.036)明显增强(t=4.279,P=0.003)。在不同性别、不同年龄组中的表达强度无显著差异(均为P0.05)。5.经Pearson相关性分析,COX-2蛋白和VEGF蛋白在鼻咽癌组中表达呈正相关(r=0.808,P=0.005)。结论1.COX-2蛋白的高表达同鼻咽癌的发生密切相关,其表达水平与患者的性别、年龄无关,与淋巴结有无转移以及TNM分期相关,有淋巴结转移的患者、TNM为Ⅲ-Ⅳ期的患者COX-2蛋白的表达强度均增强,提示COX-2蛋白的高表达同鼻咽癌的发展及转移密切相关。2.VEGF蛋白的高表达同鼻咽癌的发生密切相关,其表达水平与患者的性别、年龄无关,与淋巴结有无转移以及TNM分期相关,有淋巴结转移的患者、TNM为Ⅲ-Ⅳ期的患者VEGF蛋白的表达强度均增强,提示VEGF蛋白的高表达同鼻咽癌的发展及转移密切相关。3.COX-2蛋白和VEGF蛋白在鼻咽癌组中表达呈正相关,提示COX-2蛋白和VEGF蛋白在鼻咽癌的发生和演变中为协同作用。
[Abstract]:Objective to detect the expression of COX-2 (cyclooxygenase -2) protein and VEGF (vascular endothelial growth factor) protein in human nasopharyngeal carcinoma (nasopharyngeal carcinoma, NPC) tissues and normal nasopharyngeal mucosa, and to analyze the relationship between the expression of two proteins and NPC, the relationship between the occurrence and evolution of the two proteins in NPC and its clinical significance. Materials and methods specimens were from the nasopharyngeal endoscopic nasopharyngeal biopsies in the First Affiliated Hospital of Zhengzhou University in December -2016 February 2015. The patients were not treated with chemotherapy and non steroidal drugs before admission, and all the cases were complete. Each patient took two parts. The new biologic Department of nasopharynx, two of its nasopharynx surface smooth and unprotruded, the normal part of the naked eye. The two parts of the tissue were diagnosed as nasopharyngeal carcinoma, normal mucous tissue of the nasopharynx, and 42 patients met this condition. The nasopharynx biopsies were used as the experimental group, and the normal mucous membrane tissues of the nasopharynx were used as the experimental group. In the control group, the biopsy specimens of each nasopharyngeal carcinoma and the matched control group were quickly stored in the -80 centigrade refrigerator after the biopsy. All the patients were confirmed on the informed consent form. The experimental methods involved were the extraction of the total tissue protein, the BCA statutory egg white and the Western Blot method, which passed. The above method was used to detect the expression of COX-2 protein and VEGF protein in the experimental group and the control group. Using SPSS21.0 to analyze all the data of this article, the data obtained by the.Western Blot method need to be tested by normality, the homogeneity of variance is analyzed, the data of the normal distribution is expressed with the mean number of average numbers, the paired sample t test and the independent sample t Test, Pearson correlation analysis, P0.05 as a standard of statistical significance. Results 1. the relative expression intensity of COX-2 protein in nasopharyngeal carcinoma (0.824 + 0.147) was significantly higher than that of normal nasopharyngeal mucosa (0.201 + 0.082) (t=8.301, P=0.000).2. nasopharyngeal carcinoma (.2.) relative expression intensity of VEGF protein (0.709 + 0.103) compared with nasopharynx The normal mucosal tissue (0.368 + 0.098) significantly enhanced (t=5.343, P=0.001).3. nasopharyngeal carcinoma, the relative expression intensity of COX-2 protein in the cervical lymph node metastasis group (0.878 + 0.069) was significantly higher than that in the non cervical lymph node metastasis group (0.751 + 0.052) (t=3.310, P= 0.011), and the relative expression intensity of the COX-2 protein in the stage of TNM stage (0.8) (0.8). 82 + 0.064) was significantly enhanced (t=3.844, P=0.005) compared with stage I (0.747 + 0.046). There was no significant difference in expression intensity in different sex and age groups (P0.05). The relative expression intensity of VEGF protein in the cervical lymph node metastasis group (0.748 + 0.056) in the.4. nasopharyngeal carcinoma tissue was significantly higher than that in the non cervical lymph node metastasis group (0.656 + 0.036). (t=2.604, P=0.031), the relative expression intensity of VEGF protein in stage III - IV of TNM (0.758 + 0.038) was significantly enhanced (t=4.279, P=0.003) in phase I - II (t=4.279, P=0.003). There was no significant difference in expression intensity in different sex and age groups (P0.05).5. through Pearson correlation analysis, COX-2 protein and VEGF protein expression in nasopharyngeal carcinoma group Positive correlation (r=0.808, P=0.005). Conclusion the high expression of 1.COX-2 protein is closely related to the occurrence of nasopharyngeal carcinoma. The expression level is not related to the sex and age of the patients. It is related to the lymph node metastasis and the TNM staging. The patients with lymph node metastasis, the expression intensity of COX-2 protein in the patients with stage III to IV of TNM is enhanced, suggesting the COX-2 protein. High expression of.2.VEGF protein is closely related to the development and metastasis of nasopharyngeal carcinoma, which is closely related to the occurrence of nasopharyngeal carcinoma. The expression level is not related to the sex and age of the patients. It is related to the metastasis of the lymph nodes and the TNM staging. The patients with lymph node metastasis, the expression intensity of VEGF protein in the patients with stage III to IV of TNM is enhanced, suggesting VE The high expression of GF protein and the development and metastasis of nasopharyngeal carcinoma are closely related to the expression of.3.COX-2 protein and VEGF protein in nasopharyngeal carcinoma group, which suggests that COX-2 protein and VEGF protein are synergistic in the occurrence and evolution of nasopharyngeal carcinoma.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.63

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