丝裂霉素C-壳聚糖缓释微球抑制青光眼滤过术区瘢痕增殖的实验研究

发布时间：2018-05-25 15:33

本文选题：丝裂霉素 + 壳聚糖　；参考：《重庆医学》2016年07期

【摘要】：目的研究丝裂霉素C-壳聚糖(MMC-CS)缓释微球对兔青光眼滤过术区瘢痕增殖的疗效。方法 48只(96眼)新西兰大白兔分为4组,每组24眼,通过双眼行巩膜咬切术建立青光眼滤过术兔模型。其中24只兔的右眼术中植入MMC-CS缓释微球(A组),其左眼术中植入CS空白缓释微球(B组);另24只兔右眼术中应用含0.20mg/mL MMC棉片(C组),其左眼行单纯巩膜咬切术(D组)。术后观察各组眼压、滤过泡、前房炎性反应、并发症、角膜内皮细胞计数等情况;术后第7、14、21天分批处死各组兔模型并行病理学检查。结果 (1)眼压:A组能维持较长时间的低水平眼压,C组次之,B组与D组维持时间最短,组间差异有统计学意义(P0.01)。(2)滤过泡情况:A组滤过泡存活时间较其他各组长,差异有统计学意义(P0.01)。(3)并发症:各组中均未出现术后并发症。(4)角膜内皮细胞计数:A组术前与术后的计数值差异无统计学意义(P0.05)。(5)病理结果:A组结膜上皮完整性较好,结膜下成纤维细胞较其他各组少,滤过口周围区无明显炎症浸润,新生胶原纤维也较少。结论 MMCCS缓释微球能安全有效抑制兔青光眼滤过术区的成纤维细胞及新生胶原纤维的增殖,显著提高青光眼滤过术的成功率。
[Abstract]:Objective to study the effect of mitomycin C-chitosan MMC-CSC microspheres on scar proliferation in rabbit glaucoma filtration operation. Methods Forty-eight New Zealand white rabbits were divided into 4 groups with 24 eyes in each group. The rabbit model of glaucoma filtration was established by scleral bite in both eyes. MMC-CS sustained release microspheres were implanted into the right eyes of 24 rabbits during operation, while CS blank sustained release microspheres were implanted in the left eye in group B, while the right eyes of 24 rabbits were treated with 0.20mg/mL MMC cotton film in group C and group D with simple scleral bite in their left eyes. Intraocular pressure, filtering bleb, anterior chamber inflammatory reaction, complications and corneal endothelial cell count were observed after operation. Results (1) the intraocular pressure (IOP) group A could maintain low level IOP for a long time, the second group was group B and group D was the shortest, and the difference was statistically significant (P < 0.01). The survival time of filtering bleb in group A was longer than that in group C, and the survival time of filtering bleb in group A was longer than that in group C (P < 0.01), and the survival time of bleb in group A was longer than that in group C. There was no significant difference in corneal endothelial cell count between group A and group A before and after operation. There was no significant difference in the count of corneal endothelium between group A and group A (P 0.05). The pathological results showed that the integrity of conjunctival epithelium in group A was better than that in group A (P < 0.05). The number of subconjunctival fibroblasts was less than that of other groups. Conclusion MMCCS sustained release microspheres can effectively and safely inhibit the proliferation of fibroblasts and new collagen fibers in rabbit glaucoma filtering area, and improve the success rate of glaucoma filtration surgery.
【作者单位】：天津港口医院眼科;天津市第五中心医院眼科;天津医科大学眼科医院;
【分类号】：R779.6

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