玻璃体腔注射环孢素A对糖尿病大鼠视网膜功能及结构的保护作用

发布时间：2018-05-25 19:28

本文选题：糖尿病视网膜病变 + 玻璃体腔注射　；参考：《重庆医科大学》2012年硕士论文

【摘要】：目的：从视网膜功能及结构两方面验证：早期糖尿病（DM）大鼠即出现视网膜神经损害。探讨玻璃体腔注射环孢素A（CsA）对早期DM大鼠视网膜电流图（ERG）及视网膜组织结构、超微结构的影响。方法：健康雄性SD大鼠经链脲佐菌素诱导建立DM动物模型。实验分为正常对照组、未注射药物组（DM组）、注射CsA干预组（DM+CsA组）、注射对照液干预组（DM+DMSO组），每组9只。于建模成功后4周进行玻璃体腔注射，注射后48h应用ERG检测视网膜功能。然后处死大鼠获取视网膜，制备视网膜HE染色切片及超薄切片，，应用光镜、透射电镜检测视网膜结构变化。采用GraphPad Prism5统计学软件进行统计分析。应用采用单因素方差分析ERG结果中a波、b波的振幅及潜伏期数据。结果：（1）与正常对照组相比，3组进行不同干预的DM大鼠均有： ①ERG检测发现a波、b波振幅下降； ②视网膜切片HE染色光镜观察发现视网膜外核层疏松变薄； ③视网膜超薄切片透射电镜观察发现细胞超微结构紊乱呈凋亡表现。（2）与DM组及DM+DMSO组相比，DM+CsA组表现为： ①ERG a波、b波振幅下降程度轻（P值均＜0.05）； ②外核层疏松变薄程度轻； ③超微结构改变程度轻。结论： DM大鼠病程早期即出现视网膜神经功能的损害，并有视网膜组织结构紊乱、神经细胞超微结构改变。玻璃体腔内注射CsA可对糖尿病视网膜功能及结构起保护作用。
[Abstract]:Objective: The retinal nerve damage occurred in early diabetic rats. To investigate the effects of intravitreal injection of cyclosporine (CSA) on ERG) and the structure and ultrastructure of retina in early diabetic rats. Methods: DM model was induced by streptozotocin in healthy male SD rats. The experiment was divided into normal control group, non-injection group, CsA intervention group, DM CsA group, and control group, 9 rats in each group. Vitreous injection was performed 4 weeks after successful modeling. ERG was used to detect retinal function 48 hours after injection. Then the rats were sacrificed to obtain the retina and to prepare the HE stained and ultrathin sections of the retina. The structure of the retina was examined by light microscope and transmission electron microscope. GraphPad Prism5 statistical software was used for statistical analysis. The amplitudes and latencies of a wave / b wave in ERG results were analyzed by single factor ANOVA. Results: 1) compared with the normal control group, there were three groups of DM rats with different intervention: The amplitude of a wave B wave was decreased by 1ERG. 2HE staining of retinal sections showed that the outer nuclear layer of the retina became loose and thin; 3 the ultrastructure disorder of the cells showed apoptosis in ultrathin retina sections by transmission electron microscope (TEM). (2) compared with DM group and DM DMSO group, the findings of DM CsA group were as follows: The amplitude of 1ERG a wave and b wave decreased slightly (P < 0.05). 2the degree of loose and thinning of outer nuclear layer is light; 3 the degree of ultrastructure change was slight. Conclusion: The retinal nerve function was damaged in the early stage of diabetic rats, and the retinal tissue structure was disordered and the ultrastructure of nerve cells was changed. Intravitreal injection of CsA can protect the function and structure of diabetic retina.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R774.1

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