调亡相关基因蛋白Bcl-x_L和Bad在视网膜母细胞瘤中的表达及意义

发布时间：2018-05-26 17:00

本文选题：视网膜母细胞瘤 + 细胞凋亡　；参考：《南昌大学》2012年硕士论文

【摘要】：目的：观察比较Bcl-xL和Bad在人视网膜母细胞瘤(RB)与正常视网膜中的表达情况，并探讨两种因子表达的意义。方法：采用免疫组织化学SP法，观察比较42例RB（实验组）及15例正常视网膜组织（对照组）中Bcl-xL和Bad的表达情况，并结合临床病理资料分析二者与RB的关系。所有数据均应用SPSS for Windows17.0软件进行统计分析。结果：1、实验组Bcl-xL阳性表达率64.29%高于对照组26.67%；不同临床分型Bcl-xL阳性表达率：眼内期20%，青光眼期66.67%，眼外期88.24%，差异有统计学意义，Bcl-xL在各个RB分期中阳性表达率呈正相关；不同组织学类型Bcl-xL阳性表达率：未分化型80%，分化型41.18%，Bcl-xL在未分化型RB表达高于分化型RB；不同浸润深度Bcl-xL阳性表达率：视神经受侵犯78.57%，视神经未受侵犯35.71%，视神经受侵犯组Bcl-xL阳性表达率高于视神经未受侵犯组。 2、实验组Bad阳性表达率26.19%低于对照组60%；不同临床分型Bad阳性表达率：眼内期70%，青光眼期20%，眼外期5.88%，差异有统计学意义，Bad在各个RB分期中阳性表达率呈负相关；不同组织学类型Bad阳性表达率：未分化型8%，分化型52.94%，Bad在未分化型RB中的表达明显低于分化型RB；不同浸润深度Bad阳性表达率：视神经受侵犯14.29%，视神经未受侵犯50.00%，视神经受侵犯组Bad阳性表达率低于视神经未受侵犯组。结论：1、Bcl-xL的异常表达与RB的发生发展有关，，其表达水平随着RB恶性程度的增加而增高。 2、Bad的异常表达与RB的发生发展有关，其表达水平随着RB恶性程度的增加而降低。 3、二者可能为RB的治疗提供新途径。
[Abstract]:Aim: to investigate the expression of Bcl-xL and Bad in human retinoblastoma and normal retina. Methods: immunohistochemical SP method was used to observe and compare the expression of Bcl-xL and Bad in 42 cases of RBB (experimental group) and 15 cases of normal retina (control group). All the data were analyzed by SPSS for Windows17.0 software. Results the positive expression rate of Bcl-xL in the experimental group was 64.29% higher than that in the control group 26.67.The positive expression rate of Bcl-xL in different clinical types was 20 in intraocular phase, 66.67 in glaucoma and 88.24 in extraocular phase. The difference was positively related to the positive expression rate of Bcl-xL in all stages of RB. The positive expression rate of Bcl-xL in different histological types: 80% of undifferentiated type, 41.18% of differentiated type, and higher of Bcl-xL expression in undifferentiated RB than that of differentiated type, and the positive rate of Bcl-xL expression in different infiltrating depth was 78.57 in optic nerve, 35.71 in optic nerve, and 35.71 in optic nerve. The positive expression rate of Bcl-xL in the offending group was higher than that in the non-invasive optic nerve group. 2, the positive expression rate of Bad in the experimental group was 26.19% lower than that in the control group (60%), and the positive expression rate of Bad in different clinical types: 70 in the intraocular phase, 20 in the glaucoma stage and 5.88 in the extraocular phase. The positive expression rate of Bad in undifferentiated RB was significantly lower than that in differentiated RB, and the positive rate of Bad in different infiltrating depth was 14.29 in optic nerve, 50.004 in optic nerve. The positive expression rate of Bad in the nerve invasion group was lower than that in the optic nerve group. Conclusion the abnormal expression of Bcl-xL is related to the occurrence and development of RB, and the expression level of Bcl-xL increases with the malignancy of RB. 2the abnormal expression of Bad was related to the occurrence and development of RB, and its expression level decreased with the increase of malignant degree of RB. 3, both of them may provide a new approach for the treatment of RB.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.7

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