无血清条件下组织块法培养人角膜上皮细胞的比较

发布时间：2018-05-27 16:07

本文选题：角膜上皮细胞 + 组织块法　；参考：《眼科新进展》2017年12期

【摘要】：目的比较无血清条件下不同组织块法培养人角膜上皮细胞的出膜时间及出膜率。方法培养方式:A组:剥除角膜内皮及刮除角膜上皮后组织块角膜上皮面向上贴壁;B组:剥除角膜内皮及刮除角膜上皮后组织块角膜上皮面向下贴壁;C组:仅剥除角膜内皮后全层组织块角膜上皮面向上贴壁;D组:仅剥除角膜内皮后全层组织块角膜上皮面向下贴壁。相差显微镜观察细胞形态学变化,对比各组出膜时间及出膜率。结果 A组:细胞爬出时间(7.00±3.00)d,出膜率47.50%(19/40);B组:细胞爬出时间(7.55±2.58)d,出膜率45.83%(11/24);C组:细胞爬出时间(8.43±3.32)d,出膜率63.64%(14/22);D组:细胞爬出时间(7.49±2.20)d,出膜率72.22%(39/54)。卡方检验结果示,A组与D组、B组与D组出膜率相比,差异均有统计学意义(均为P0.05),余组间两两比较差异均无统计学意义(均为P0.05)。结论无血清条件下不同组织块培养方式细胞爬出时间大致相同,约一周。相比较而言,仅剥除角膜内皮后全层组织块角膜上皮面向下贴壁培养法出膜率明显较高,且培养的细胞更纯、更稳定。
[Abstract]:Objective to compare the time and rate of membrane production of human corneal epithelial cells cultured in serum-free medium with different tissue mass methods. Methods: group A: group B: peeling corneal endothelium and scraping corneal epithelium facing to the upper wall: group B: group C: exfoliating corneal endothelium and scraping corneal epithelium facing to the lower wall: group C: exfoliation of corneal epithelium In group D, the corneal epithelium of the whole layer of corneal endothelium was only peeling off the corneal endothelium, and the whole layer of corneal epithelium was facing to the lower wall after the corneal endothelium was removed. Phase contrast microscope was used to observe the changes of cell morphology, and the time and rate of membrane formation were compared. Results in group A, the time of cell climbing was 7.00 卤3.00 days, the rate of membrane exudation was 47.50 and the time of cell climbing was 7.55 卤2.58 days, and the rate of membrane exudation was 45.833 and 118.43 卤3.32 days, respectively. In group D, the time of cell climbing was 7.49 卤2.20 days, and the rate of membrane exit was 72.2222 / 54.The time of cell climbing was 8.43 卤3.32 days, and that of group D was 63.64 卤3.32 days, and that of group D was 7.49 卤2.20 days, and the rate of membrane exudation was 72.2222%. The results of chi-square test showed that there were significant differences in the rate of membrane production between group A and group D (P 0.05), but there was no significant difference between the other two groups (all P 0.05). Conclusion under serum-free condition, the cell crawling out time of different tissue mass culture is about the same, about one week. In contrast, the rate of membrane formation was significantly higher in the whole layer of corneal epithelium after only corneal endothelium was stripped, and the cultured cells were more pure and stable.
【作者单位】：厦门大学附属厦门眼科中心;郑州大学第一附属医院眼科;
【分类号】：R779.65

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