白介素17C在鼻息肉中的表达及其意义

发布时间：2018-06-03 06:59

本文选题：慢性鼻-鼻窦炎伴鼻息肉 + 白细胞介素17C　；参考：《延边大学》2017年硕士论文

【摘要】：目的:通过观察白细胞介素17C(interleukin17C-IL,17C)在鼻息肉中的表达,探讨IL-17C在慢性鼻-鼻窦炎伴鼻息肉发病过程中的作用机制。方法:收集2016年1月-2016年6月于我院耳鼻咽喉头颈外科就诊并行鼻内窥镜下鼻窦手术治疗的慢性鼻-鼻窦炎伴鼻息肉(chronic rhinosinusitis with nasal polyps,CRSwNPs)患者的鼻息肉标本50例(鼻息肉组),同期住院的鼻中隔偏曲患者行鼻申隔成形术并切除的申鼻甲膜标本10例(对照组)。根据HE染色结果,将鼻息肉分为嗜酸性粒细胞型和非嗜酸性粒细胞型。应用免疫组织化学染色法检测IL-17C在鼻息肉组织以及对照组黏膜中的表达,分析不同类型鼻息肉中IL-17C表达水平的相关性。结果:1.在鼻息肉组中,嗜酸性粒细胞型鼻息肉24例(48%),非嗜酸性粒细胞型鼻息肉26例(52%)。2.IL-17C主要在鼻息肉组织的上皮细胞申表达。IL-17C在鼻息肉组的表达水平明显高于对照组,IL-17C在嗜酸性粒细胞型鼻息肉中的阳性表达率为87.5%,在非嗜酸性粒细胞型中的阳性率为73.1%,在对照组中的阳性率为20%,嗜酸性粒细胞型鼻息肉及非嗜酸性粒细胞型分别与对照组相比,存在显著的统计学差异(P0.01)。3.IL-17C在嗜酸性粒细胞型鼻息肉的上皮细胞中的表达强度评分高于非嗜酸性粒细胞型,差异具有统计学意义(P0.05)。结论：IL-17C是参与CRSwNPs发病的重要细胞因子,有望成为鼻息肉治疗的一个新的靶向。
[Abstract]:Aim: to investigate the expression of interleukin17C-IL17C in nasal polyps and to explore the role of IL-17C in the pathogenesis of chronic rhinosinusitis and nasal polyps. Methods: from January 2016 to June 2016, 50 nasal polyps (nasal polyps) were collected from 50 patients with chronic rhinosinusitis and nasal polyps treated by endoscopic sinus surgery in our hospital from January 2016 to June 2016. In group A, 10 specimens of turbinate membrane were treated with nasal septoplasty and excision of nasal septum deviation in the same period (control group). According to HE staining, nasal polyps were divided into eosinophil type and non-eosinophil type. Immunohistochemical staining was used to detect the expression of IL-17C in nasal polyps and control mucosa, and the correlation of IL-17C expression in different types of nasal polyps was analyzed. The result is 1: 1. In nasal polyps, 24 cases of eosinophil nasal polyps and 26 cases of non-eosinophil nasal polyps showed that IL-17C was mainly expressed in epithelial cells of nasal polyps. The expression level of IL-17C in nasal polyps was significantly higher than that in control group. The positive expression rate of nasal polyps was 87.5%, the positive rate of non-eosinophil type was 73.1%, the positive rate of control group was 20%, the positive rate of eosinophil type nasal polyp and non-eosinophilic granulocyte type were respectively compared with the control group. There was significant statistical difference in the expression of IL-17C in the epithelial cells of eosinophil type nasal polyps, which was significantly higher than that of non-eosinophil type, and the difference was statistically significant (P 0.05). Conclusion: 1-IL-17 C is an important cytokine involved in the pathogenesis of CRSwNPs, and it is expected to be a new target for the treatment of nasal polyps.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R765

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