P-EGFR、P-ERK和NF-κB在中耳胆脂瘤上皮中的表达和意义

发布时间：2018-06-05 15:27

本文选题：中耳 + 胆脂瘤　；参考：《中华耳科学杂志》2017年03期

【摘要】：目的研究磷酸化表皮生长因子受体(P-EGFR)、磷酸化细胞外信号调节激酶(P-ERK)和细胞核转录因子κB(NF-κB)在中耳胆脂瘤上皮组织中的表达情况,探讨EGFR/ERK/NF-κB信号通路在中耳胆脂瘤发病机制中的作用。方法应用免疫组织化学SP法及Western blot(WB)免疫印迹法检测30例中耳胆脂瘤标本及15例正常外耳道皮肤组织中P-EGFR、P-ERK和NF-κB蛋白的表达。结果(1)P-EGFR蛋白阳性表达主要定位于细胞质和(或)细胞膜,在胆脂瘤组的阳性表达率为63%,明显高于正常外耳道皮肤组的20%(P0.05);P-ERK蛋白阳性表达主要定位于细胞质和(或)细胞核,在胆脂瘤组的阳性表达率为70%,明显高于正常外耳道皮肤组的20%(P0.05);NF-κB蛋白阳性表达主要定位于细胞核,在胆脂瘤组的阳性表达率为60%,明显高于正常耳道皮肤组的13.3%(P0.05)。在30例中耳胆脂瘤组织中,P-EGFR、P-ERK和NF-κB两两之间呈正相关关系(P0.05)。(2)Western blot免疫印迹法检测结果显示:中耳胆脂瘤组中P-EGFR、P-ERK和NF-κB的表达量明显高于正常外耳道皮肤组的表达量。结论 P-EGFR、P-ERK和NF-κB在中耳胆脂瘤上皮中表达增强,EGFR/ERK/NF-κB信号通路可能在中耳胆脂瘤组织的形成和发展过程中起重要作用。
[Abstract]:Objective to investigate the expression of phosphorylated epidermal growth factor receptor P-EGFRN, phosphorylated extracellular signal-regulated kinase P-ERK (P-ERK) and nuclear transcription factor 魏 B (NF- 魏 B) in middle ear cholesteatoma, and to investigate the role of EGFR / ERK / NF- 魏 B signaling pathway in the pathogenesis of middle ear cholesteatoma. Methods the expression of P-EGFRP-ERK and NF- 魏 B protein in 30 cases of middle ear cholesteatoma and 15 cases of normal skin tissue of external auditory canal were detected by immunohistochemical SP method and Western blotl Western blotting. Results the positive expression of P-EGFR protein was mainly located in the cytoplasm and (or) cell membrane. The positive expression rate of P-EGFR protein in cholesteatoma group was 63, which was significantly higher than that in the normal external auditory canal skin group. The positive expression of P-EGFR protein was mainly located in the cytoplasm and / or nucleus. The positive expression rate of NF- 魏 B in the cholesteatoma group was 70, which was significantly higher than that in the normal external auditory canal skin group. The positive expression of NF- 魏 B was mainly located in the nucleus. The positive expression rate of NF- 魏 B protein in the cholesteatoma group was 60 and was significantly higher than that in the normal auditory canal skin group (13. 3 and P 0. 05). There was a positive correlation between P-EGFRP-ERK and NF- 魏 B in 30 cases of middle ear cholesteatoma. The expression of P-EGFRP-ERK and NF- 魏 B in middle ear cholesteatoma group was significantly higher than that in normal external auditory canal skin group. Conclusion the expression of P-EGFRP-ERK and NF- 魏 B in the epithelium of middle ear cholesteatoma may play an important role in the formation and development of middle ear cholesteatoma.
【作者单位】：湖南省妇幼保健院儿童五官科;中南大学湘雅医院耳鼻咽喉头颈外科;河南科技大学第一附属医院耳鼻咽喉头颈外科;中南大学湘雅二医院耳鼻咽喉头颈外科中南大学耳科研究所;
【基金】：国家自然科学基金(项目批准号:81400457)~~
【分类号】：R764.21

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