骨髓间充质干细胞静注对碱烧伤兔角膜修复的促进作用及机制

发布时间：2018-06-06 10:14

本文选题：角膜修复 + 碱烧伤　；参考：《山东医药》2017年20期

【摘要】：目的观察骨髓间充质干细胞(BMSCs)静注对碱烧伤兔角膜修复的促进作用并探讨其机制。方法取新西兰大白兔60只,制备右眼角膜缘碱烧伤模型,随机分为观察组和对照组,各30只(30眼)。观察组耳缘静脉注射BMSCs细胞悬液3.47×10~6个/kg,对照组注射等体积PBS。注射后第3、21、60天,两组行右眼角膜透明度和角膜上皮再生状况评分,采用裂隙生物显微镜观察角膜血管新生情况,计算角膜新生血管面积。结果第3、21、60天观察组角膜上皮再生程度评分均低于对照组(P均0.05)。第3天两组角膜混浊程度评分比较差异无统计学意义(P0.05),第21、60天观察组均低于对照组(P均0.05)。观察组第3、21天角膜新生血管面积大于对照组(P均0.05),第60天小于对照组(P均0.05)。结论 BMSCs静注可促进碱烧伤兔角膜的修复,提高角膜透明度;其机制可能与其早期促进损伤角膜新生血管生成、晚期抑制角膜新生血管生成有关。
[Abstract]:Objective to investigate the effect of bone marrow mesenchymal stem cells (BMSCs) on corneal repair in alkali burn rabbits. Methods 60 New Zealand rabbits were randomly divided into two groups: the experimental group (30 eyes) and the control group (30 eyes). The suspension of BMSCs cells was injected intravenously into the ear margin of the observation group (3.47 脳 10 ~ 6 / kg), and the control group was injected with the same volume of BMSCs. The corneal diaphaneity and corneal epithelium regeneration status of the two groups were evaluated on the 3rd day and the 21st day after injection. The corneal angiogenesis was observed with a fissure biomicroscope and the corneal neovascularization area was calculated. Results the grade of corneal epithelium regeneration in the observation group was lower than that in the control group (P < 0.05). On the 3rd day, there was no significant difference in the corneal opacity score between the two groups (P 0.05), but the observation group was lower than the control group (P 0.05) on the 21st day. The area of corneal neovascularization in the observation group was larger than that in the control group on day 3 and 21, respectively, and the area of corneal neovascularization in the observation group was smaller than that in the control group on day 60. Conclusion intravenous injection of BMSCs can promote corneal repair and improve corneal transparency in rabbits with alkali burn, and its mechanism may be related to its early promotion of corneal neovascularization and late inhibition of corneal neovascularization.
【作者单位】：天津医科大学眼科临床学院天津市眼科医院;武警后勤学院附属医院;天津医科大学眼科中心;
【基金】：天津市应用基础及前沿技术研究项目(15JCYBJC26500)
【分类号】：R772.2

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