川芎嗪对慢性高眼压大鼠视网膜神经节细胞凋亡的影响

发布时间：2018-06-09 04:52

本文选题：高眼压 + 视网膜神经节细胞　；参考：《眼科新进展》2013年08期

【摘要】：目的研究川芎嗪对慢性高眼压大鼠视网膜神经节细胞(retina ganglion cells,RGC)凋亡的保护作用。方法健康SD大鼠30只,随机分为正常组、高眼压组和川芎嗪治疗组,每组10只。高眼压组和治疗组分别采用上巩膜静脉烧灼法制作高眼压模型,正常对照组不做处理。造模成功后7d治疗组给予川芎嗪腹腔注射,正常组与高眼压组给予等量的生理盐水腹腔注射。造模3周后取三组大鼠眼球,行视网膜HE染色、TUNEL原位细胞凋亡检测和Bcl-2免疫组织化学检测。结果造模后高眼压组、川芎嗪治疗组各时间点眼压与正常组比较,差异均有统计学意义(均为P0.05),而两组间各时间点比较,差异均无统计学意义(均为P0.05)。正常组、高眼压组、川芎嗪治疗组大鼠视网膜RGC层TUNEL阳性细胞数分别为0、9.00±0.89、3.50±1.89,三组间差异有统计学意义(F=86.160,P=0.000);三组间两两比较,差异均有统计学意义(均为P=0.000)。三组大鼠RGC层Bcl-2阳性表达光密度值分别为0.373±0.090、0.375±0.146、0.390±0.268,三组差异有统计学意义(F=84.658,P=0.000);三间组两两比较,差异均有显著统计学意义(均为P=0.000)。结论川芎嗪通过上调Bcl-2蛋白的表达可明显抑制慢性高眼压大鼠RGC凋亡,从而发挥RGC保护作用。
[Abstract]:Objective to study the protective effect of ligustrazine on apoptosis of retinal ganglion cells (RGCs) in rats with chronic intraocular hypertension. Methods 30 healthy Sprague-Dawley rats were randomly divided into normal group, intraocular hypertension group and ligustrazine treatment group with 10 rats in each group. The model of high intraocular pressure was made by scleral vein cauterization in the high intraocular pressure group and the treatment group, but not in the normal control group. The rats in the treatment group were intraperitoneally injected with ligustrazine, and the normal group and the high intraocular pressure group were intraperitoneally injected with the same amount of normal saline on the 7th day after the successful establishment of the model. After 3 weeks, the eyeballs of the three groups were taken out, and the in situ apoptosis and Bcl-2 immunohistochemistry were detected by HE staining and Tunel in the retina. Results there were significant differences in intraocular pressure (IOP) between the two groups at different time points (P 0.05), but there was no significant difference between the two groups at each time point (P 0.05). The number of Tunel positive cells in RGC layer of rats in normal group, high intraocular pressure group and ligustrazine treatment group was 0 9. 00 卤0. 89 卤0. 50 卤1. 89, respectively. The difference among the three groups was statistically significant (P 0. 000). The positive optical density of Bcl-2 expression in RGCs of the three groups was 0.373 卤0.090 卤0.375 卤0.146 卤0.390 卤0.268 respectively, and the difference among the three groups was statistically significant (P < 0.01). Conclusion Ligustrazine can obviously inhibit the apoptosis of RGC in rats with chronic intraocular hypertension by up-regulating the expression of Bcl-2 protein, thus exerting the protective effect of RGC.
【作者单位】：新乡医学院第三附属医院眼科;河南省洛阳市第三人民医院;
【基金】：河南省卫生厅科技攻关计划(编号:2011020102) 新乡医学院研究生科研创新支持计划(编号:YJSCX201009Y) 新乡医学院重点学科资助项目(编号:ZD200982)~~
【分类号】：R775

【相似文献】