组织因子（TF）在喉癌侵袭转移中的相关性研究

发布时间：2018-06-10 07:02

本文选题：喉癌 + 癌组织　；参考：《武汉大学》2016年博士论文

【摘要】：第一部分组织因子(TF)在喉癌侵袭转移中的相关性研究目的为了探求组织因子TF和喉癌之间的关系,我们对喉癌病人的癌组织(carcinomatous tissues, CT)和癌旁组织(pericarcinomatous tissues, PT) TF的表达差异进行了分析研究,并探讨了其临床的潜在意义。方法利用GEO数据分析、免疫组化、Western-Blot和real-time RT-PCR共同分析和检测,TF蛋白在喉癌病人(n=20)的癌旁组织及癌组织中的表达情况。结果TF蛋白在喉癌病人(n=20)的癌旁组织中高表达,癌组织中低表达或不表达,两者存在极其显著的统计学差异(P0.01)。同时,TF的mRNA在癌旁组织中表达量也显著高于癌组织(P0.01)。GEO数据分析TF在肿瘤组织中的表达与远处正常组织表达水平无差异(P0.05),却显著低于肿瘤切缘组织中的表达水平(P0.01)。另外,TF表达水平低的头颈部鳞癌患者术后的无病生存时间显著长于TF表达水平高的患者。结论TF可能有助于喉癌的癌变,也可以作为判定喉癌恶性程度和进一步发展的标志物,为人类治疗和认知喉癌提供了一条新的思路。第二部分喉癌细胞胞外体诱导血管内皮细胞表达TF的机制研究目的：探讨喉癌细胞胞外体在喉癌转移侵袭中的作用,并进一步阐述胞外体诱导TF表达促进喉癌转移的分子机制。方法：培养人喉癌细胞Hep-2和人血管内皮细胞HUVEC,在HUVEC细胞中加入Hep-2细胞胞外体培养,检测TF蛋白及mRNA的改变,同时利用CCK-8增殖实验、划痕实验和transwell迁移实验检测胞外体对血管内皮细胞增殖和迁移的改变。结果：HUVEC细胞加入肿瘤胞外体后,TF蛋白及RNA表达水平明显增高(P0.01),同时HUVEC细胞增殖明显增强(P0.05),迁移能力亦明显增加。结论：喉癌细胞分泌的胞外体可以在体外显著刺激HUVEC细胞高表达TF,并显著增强HUVEC细胞的增殖和迁移能力。
[Abstract]:The relationship between tissue Factor TF and Laryngeal carcinoma in invasion and Metastasis objective to explore the relationship between tissue factor TF and laryngeal carcinoma. The difference of expression of CTs and PTTF in patients with laryngeal carcinoma was studied and its clinical significance was discussed. Methods the expression of TF protein in the adjacent tissues and tissues of laryngeal cancer patients was analyzed and detected by using GEO data, immunohistochemical staining and real-time reverse transcription polymerase chain reaction (RT-PCR). Results TF protein was highly expressed in the paracancerous tissues of patients with laryngeal carcinoma, but it was low or not expressed in the carcinoma tissues. There was a significant statistical difference between the two groups (P 0.01). At the same time, the expression of TF mRNA in paracancerous tissues was significantly higher than that in cancer tissues P0.01. GEO data. There was no difference between the expression level of TF mRNA in tumor tissues and that in distant normal tissues. However, the expression level of TF mRNA was significantly lower than that in tumor incised margin tissues (P0.01). In addition, the disease-free survival time of head and neck squamous cell carcinoma with low TF expression was significantly longer than that of high TF expression. Conclusion TF may be helpful to the carcinogenesis of laryngeal carcinoma, and can also be used as a marker for judging the malignant degree and further development of laryngeal carcinoma, which provides a new idea for human treatment and cognition of laryngeal carcinoma. Part two the mechanism of expression of TF in vascular endothelial cells induced by extracellular bodies of laryngeal carcinoma objective: to investigate the role of extracellular bodies in the metastasis and invasion of laryngeal carcinoma and to further elucidate the molecular mechanism of TF expression induced by extracellular bodies in promoting the metastasis of laryngeal carcinoma. Methods: human laryngeal carcinoma cells Hep-2 and human vascular endothelial cells HUVECs were cultured in vitro. The changes of TF protein and mRNA were detected by CCK-8 proliferation assay. The effects of extracellular bodies on proliferation and migration of vascular endothelial cells were detected by scratch test and transwell migration assay. Results the expression levels of TF protein and RNA in human HUVEC cells were significantly higher than those in the tumor extracellular bodies, while the proliferation and migration of HUVEC cells were significantly increased. Conclusion: the extracellular bodies secreted by laryngeal carcinoma cells can significantly stimulate the overexpression of TFVEC cells and enhance the proliferation and migration of HUVEC cells in vitro.
【学位授予单位】：武汉大学
【学位级别】：博士
【学位授予年份】：2016
【分类号】：R739.65

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