角质形成细胞在中耳胆脂瘤过度增殖中的作用

发布时间：2018-06-13 16:45

本文选题：中耳胆脂瘤 + 血管内皮生长因子　；参考：《临床耳鼻咽喉头颈外科杂志》2016年02期

【摘要】：目的:研究基质金属蛋白酶9(MMP9)、血管内皮生长因子(VEGF)、角质形成细胞生长因子(KGF)及角质形成细胞生长因子受体(KGFR)在中耳胆脂瘤上皮中的表达,探讨它们与角质形成细胞之间的相互作用及在中耳胆脂瘤上皮过度增殖中的作用。方法:通过免疫组织化学方法检测MMP9、VEGF、KGF、KGFR、增殖细胞核抗原(Ki-67)在50例中耳胆脂瘤组织和15例正常外耳道皮肤中的表达,以Ki-67作为胆脂瘤细胞增殖活性的标记物,来评估胆脂瘤上皮中角质形成细胞的增殖状态。结果:1中耳胆脂瘤上皮中VEGF和MMP9的平均光密度值高于正常外耳道皮肤,差异有统计学意义(均P0.01)。2KGF及KGFR在中耳胆脂瘤组织中的表达量明显高于正常外耳道皮肤,差异具有统计学意义(均P0.01);随着胆脂瘤上皮中KGF及KGFR平均光密度值增加,Ki-67表达强度也相应地增加。3在MMP9和VEGF均阳性的胆脂瘤组织中,随着MMP9及VEGF平均光密度值增加,胆脂瘤组织中KGF表达也相应地增加。结论:MMP9、VEGF、KGF及KGFR蛋白在中耳胆脂瘤组织的高度增殖中起重要作用,并且VEGF、MMP9与KGF蛋白在胆脂瘤组织中的增殖中可能起协同作用。
[Abstract]:Objective: to study the expression of matrix metalloproteinase 9 (MMP9), vascular endothelial growth factor (VEGF), keratinocyte growth factor (KGF) and keratinocyte growth factor receptor (KGFR) in middle ear cholesteatoma. To investigate their interaction with keratinocytes and their role in the proliferation of middle ear cholesteatoma epithelium. Methods: the expression of MMP9 VEGFGF-KGFR and proliferating cell nuclear antigen Ki-67 in 50 cases of middle ear cholesteatoma and 15 cases of normal external auditory canal skin were detected by immunohistochemical method. Ki-67 was used as the marker of proliferation activity of cholesteatoma cells. To assess the proliferation of keratinocytes in cholesteatoma epithelium. Results the mean optical density of VEGF and MMP9 in the epithelium of middle ear cholesteatoma was significantly higher than that in normal skin of external auditory canal (P0.01KGF and KGFR) in middle ear cholesteatoma tissue. With the increase of the mean optical density of KGF and KGFR in cholesteatoma epithelium, the expression intensity of Ki-67 was increased correspondingly. 3. In cholesteatoma tissues with MMP9 and VEGF positive, the mean optical density of MMP9 and VEGF increased with the increase of MMP9 and VEGF. The expression of KGF in cholesteatoma was also increased accordingly. Conclusion VEGFGF-KGF and KGFR proteins play an important role in the high proliferation of middle ear cholesteatoma, and VEGF MMP9 and KGF protein may play a synergistic role in the proliferation of cholesteatoma.
【作者单位】：福建医科大学附属宁德市医院耳鼻咽喉科宁德市眼耳鼻咽喉科研究所;福建医科大学附属第一医院耳鼻咽喉科;
【基金】：宁德市科技局指令性课题(No:宁科2011-0043)
【分类号】：R764.21

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