提前光照刺激对早产近视小鼠视网膜中capase-3表达的影响

发布时间：2018-06-17 12:28

本文选题：早产儿 + 近视　；参考：《眼科新进展》2017年09期

【摘要】：目的观察早产近视小鼠视网膜的凋亡情况以及caspase-3蛋白的表达变化,探讨早产近视的发病机理。方法选取实验用新生C57BL/6J小鼠共60只,随机分成3组(P6开睑组、P10开睑组和自然开睑组),每组各20只。P6开睑组和P10开睑组分别于新生6 d龄和10 d龄处理右眼,使其睁眼接受提前光照,左眼为自身对照不作处理,自然开睑组小鼠眼睑待P14时自然睁开。所有小鼠于P15检影验光获得双眼屈光度数,采用电子数显千分尺测量眼轴长度,TUNEL法检测各组小鼠视网膜细胞的凋亡,免疫组织化学方法和Western-blot检测小鼠视网膜中caspase-3蛋白的表达。结果 P6开睑组小鼠形成了(-7.55±0.15)D的相对近视,P10开睑组小鼠形成了(-5.25±0.10)D的相对近视,自然开睑组小鼠未形成相对近视,三组比较差异有统计学意义(P0.05);P6开睑组右眼眼轴长度(2.49±0.08)mm及P10开睑组小鼠右眼眼轴长度(2.51±0.03)mm均明显短于自然开睑组右眼眼轴长度(2.58±0.04)mm,三组比较差异有统计学意义(P0.05)。免疫组织化学结果显示:caspase-3阳性细胞主要表达于P6开睑组和P10开睑组小鼠视网膜内核层和神经节细胞层。TUNEL结果显示,P6开睑组和P10开睑组小鼠视网膜视神经节细胞层可见棕黄色凋亡细胞。Western-blot结果显示caspase-3蛋白P6开睑组(灰度值52.70%)和P10开睑组(灰度值35.76%)小鼠视网膜中的表达量上调。结论提前光照刺激可诱导小鼠形成相对近视,接受光照的时间越早,小鼠形成的相对近视度数越高;提前光照刺激小鼠近视形成过程中,神经节细胞和内核层细胞发生凋亡,caspase-3蛋白参与凋亡的发生。
[Abstract]:Objective to observe the changes of retinal apoptosis and caspase-3 protein expression in premature myopia mice and to explore the pathogenesis of premature myopia. Methods A total of 60 newborn C57BL / 6J mice were randomly divided into 3 groups: P10 open eyelid group and natural open eyelid group. The right eyes were treated in each group at the age of 6 days and 10 days, respectively. The left eye was untreated by self-control, and the eyelid of the mice in the open eyelid group was opened naturally when P14 was treated. The diopter of both eyes was obtained by P15 optometry. The apoptosis of retinal cells in each group was detected by electron digital micrometer and the expression of caspase-3 protein in the retina was detected by immunohistochemistry and Western-blot. Results the relative myopia of mice in the P6 blepharoopening group was 7.55 卤0.15 D and that in the P10 group was 5.25 卤0.10D, but the relative myopia was not found in the natural eyelid opening group. There were significant differences among the three groups. The length of the right eye axis in the open eyelid group was 2.49 卤0.08)mm and 2.51 卤0.03)mm in the P10 open eyelid group, which was significantly shorter than that in the natural eyelid opening group (2.58 卤0.04 mm), and the difference among the three groups was significant (P0.05). The results of immunohistochemistry showed that the positive cells of Caspase-3 were mainly expressed in the nuclear layer and ganglion cell layer of retina of mice in P6 blepharoplasty group and P10 blepharoplasty group. Tunel results showed that P6 open eyelid group and P10 open eyelid group mice retinal optic ganglion cell layer. The results of brown-yellow apoptotic cells. Western-blot showed that the expression of caspase-3 protein in the retina was up-regulated in the open eyelid group (gray value 52.70) and the P10 open eyelid group (35.766-10). Conclusion early illumination stimulation can induce relative myopia in mice, and the earlier the exposure time is, the higher the relative myopia degree of mice is. Apoptosis occurs in ganglion cells and nuclear layer cells. Caspase-3 protein is involved in apoptosis.
【作者单位】：武汉大学人民医院眼科;
【基金】：湖北省科技支撑计划项目(编号:2015BCA311)~~
【分类号】：R774.1

【相似文献】