血管内皮生长因子及对氧磷酶1基因多态性与糖尿病视网膜病变的相关性

发布时间：2018-06-18 22:33

本文选题：血管内皮生长因子 + 对氧磷酶　；参考：《重庆医学》2017年30期

【摘要】：目的探讨血管内皮生长因子(VEGF)、对氧磷酶1(PON1)基因多态性与糖尿病视网膜病变(DR)的相关性。方法选取2013年1月至2015年12月西宁市第二人民医院收治的糖尿病(DM)患者168例,将其分为DM组(48例)、增殖性DR(PDR)组(66例)和非增殖性DR(NPDR)组(54例);另选取同期体检健康者104例作为对照组(NC组)。通过聚合酶链反应-限制性片段长度多态性(PCR-RFLP)测定血管内皮生长因子(VEGF)和对氧磷酶1(PON1)基因多态性,采用紫外分光光度计检测所有受试者超氧化歧化酶(SOD)和丙二醛(MDA)水平。结果与NC组比较,DM组、NPDR组及PDR组收缩压、糖化血红蛋白水平明显升高,差异均有统计学意义(P0.05);而舒张压、胆固醇、三酰甘油、高密度脂蛋白水平比较,差异均无统计学意义(P0.05)。PDR组和NPDR组收缩压比较,差异有统计学意义(P0.05),而舒张压、胆固醇、三酰甘油、高密度脂蛋白、糖化血红蛋白水平比较,差异均无统计学意义(P0.05)。CC、CT、TT基因型患者DR发生率分别为71.05%、56.27%、38.64%,CC基因型患者DR发生率明显高于CT和TT基因型患者,差异有统计学意义(P0.05)。PON1 192位点不同基因型健康体检者SOD、MDA水平比较,差异均无统计学意义(P0.05),QQ基因型DR患者体内SOD水平低于QR、RR基因型患者,MDA水平高于QR、RR基因型患者,差异均有统计学意义(P0.05)。结论 VEGF、PON1基因的表达影响DR的发生和进展,PON1基因可能通过影响DR患者体内氧化应激酶的表达控制疾病的发展。
[Abstract]:Objective to investigate the association between the polymorphism of vascular endothelial growth factor (VEGF) VEGFN, paraoxonase 1 (PON1) gene and diabetic retinopathy (DRN). Methods 168 patients with DM were selected from January 2013 to December 2015 in Xining second people's Hospital. They were divided into DM group (n = 48), proliferative DRN group (n = 66) and non-proliferative DRN DRN group (n = 54), and 104 healthy subjects as control group. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphism of vascular endothelial growth factor (VEGF) and paraoxonase 1 (PON1) gene, and the levels of superoxide dismutase (Sod) and malondialdehyde (MDAs) were detected by ultraviolet spectrophotometer. Results compared with NC group, systolic blood pressure (SBP) and glycosylated hemoglobin (HbA1c) were significantly increased in DM group (NPDR group) and PDR group (P 0.05), while diastolic blood pressure (DBP), cholesterol (TC), triglyceride (TG) and high density lipoprotein (HDL) levels were compared. There was no significant difference in systolic blood pressure (SBP) between PDR group and NPDR group, but diastolic blood pressure, cholesterol, triacylglycerol, high density lipoprotein and glycosylated hemoglobin were compared. The incidence of Dr in patients with CC genotype was significantly higher than that in patients with CT and TT genotypes, and there was a significant difference in the levels of MDA between patients with CC genotype and those with genotype CT and TT genotypes. There was significant difference in the levels of MDA in patients with different genotypic health controls at P0.05N. PON1 192 locus. The incidence of Dr in patients with CC genotype was 71.05 and 56.27, respectively, which was significantly higher than that in patients with CT and TT genotypes. The level of SOD in patients with QQ genotype Dr was lower than that in patients with QRNRR genotype, and the level of MDA in patients with QRNRR genotype was higher than that in patients with QRNRR genotype, and the difference was statistically significant (P 0.05). Conclusion the expression of VEGFG PON1 gene may affect the pathogenesis and progression of Dr. The expression of PON1 gene may control the development of disease by affecting the expression of ERK in Dr patients.
【作者单位】：西宁市第二人民医院眼科;青海大学医学院藏医学院藏医系;
【基金】：国家自然科学基金资助项目(81560806)
【分类号】：R587.2;R774.1

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