双氢青蒿素对人下咽癌裸鼠移植瘤的抑制作用

发布时间：2018-06-21 15:47

本文选题：双氢青蒿素 + 下咽癌　；参考：《肿瘤防治研究》2017年10期

【摘要】：目的明确不同浓度双氢青蒿素(DHA)抑制人下咽癌细胞Fadu裸鼠移植瘤生长的效应。方法细胞克隆形成实验分析DHA体外抑制肿瘤形成能力;建立Fadu裸鼠移植瘤模型,腹腔注射低、中、高浓度DHA作为DHA处理组,腹腔注射顺铂作为化疗组;测量移植瘤的体积,干预3周后剥离瘤块,判断不同浓度DHA抑瘤率及抑瘤效果;根据体重变化及肝、脾、肾的重量变化,判断DHA是否有潜在的毒性。结果 (1)DHA抑制Fadu细胞的体外生长;(2)DHA低(25 mg/kg)、中(50mg/kg)、高(100 mg/kg)剂量组的抑瘤率分别为39.74%、60.52%、78.70%,顺铂组的抑瘤率为79.22%;(3)DHA对裸鼠体重及肾重量无影响,能增加荷瘤裸鼠的肝、脾重量。结论低、中剂量DHA虽能抑制Fadu移植瘤生长,但抑瘤效果不如顺铂;高剂量DHA抑瘤效果与顺铂相同,且没有明显的肝、肾、脾毒性作用。
[Abstract]:Objective to investigate the inhibitory effect of dihydroartemisinin (DHA) on the growth of human hypopharyngeal carcinoma cell line Fadu in nude mice. Methods the ability of DHA to inhibit tumor formation in vitro was analyzed by cell clone formation assay, and the model of Fadu xenograft tumor was established, in which low, middle and high concentration DHA was injected intraperitoneally as DHA treatment group, and cisplatin was injected intraperitoneally as chemotherapy group, and the volume of transplanted tumor was measured. After 3 weeks of intervention, tumor blocks were removed to determine the inhibitory rate and effect of DHA at different concentrations, and the potential toxicity of DHA was determined according to the changes of body weight and weight of liver, spleen and kidney. Results the inhibitory rates of DHA on Fadu cell growth in vitro were 25 mg / kg, 50 mg / kg and 100 mg / kg, respectively. The inhibition rates of DHA in DHA group were 39.74 mg / kg, 60.52mg / kg, 78.70 mg / kg, respectively. The inhibition rate of DHA in cisplatin group was 79.22 DHA and had no effect on the weight of nude mice and kidney, but could increase the weight of liver and spleen of tumor-bearing nude mice. Conclusion low and middle dose DHA can inhibit the growth of Fadu transplanted tumor, but its inhibitory effect is not as good as cisplatin, and high dose DHA has the same effect as cisplatin, and has no obvious toxic effect on liver, kidney and spleen.
【作者单位】：白求恩国际和平医院耳鼻喉头颈外科;河北中医学院免疫学与病原生物学教研室;
【基金】：2016年度中国博士后科学基金面上项目(2016M603018) 河北省自然科学基金(H2017423011) 河北省大学生创新创业训练计划(201614432031)
【分类号】：R739.63

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