CR2、MLXIP、ZNF608和CSF1R在鼻咽癌组织中的表达及其临床意义

发布时间：2018-06-26 00:33

本文选题：鼻咽癌 + 补体受体2　；参考：《广西医科大学》2012年硕士论文

【摘要】：背景和目的：在前期的研究中，我们通过利用能够检测14112个人类基因的cDNA芯片，对20例鼻咽癌患者组织样本与15例鼻咽慢性炎症患者组织样本进行表达谱分析比较，找出在鼻咽癌组织中有明显差异表达的基因。本实验的研究目的是：1、从中选出鼻咽癌相关基因补体受体2（CR2）、MLX相互作用的蛋白（MLXIP），在50例鼻咽癌组织中进行RT-PCR，验证其是否与基因芯片表达结果相一致；2、从中选出放射敏感相关基因锌指蛋白608（ZNF608）、集落刺激因子1受体（CSF1R），在17例鼻咽癌组织中进行RT-PCR，验证其是否与基因芯片表达结果相一致；3、分析CR2、MLXIP、ZNF608和CSF1R这四个基因在鼻咽癌组织中的表达与临床的相关性。方法：实验分别对50例、17例低分化鼻咽癌组织进行荧光实时定量RT-PCR，验证CR2、MLXIP、ZNF608和CSF1R在鼻咽癌组织中的表达，并将其与临床进行相关性分析。结果： 1、我们选用CR2和MXLIP在50例低分化鼻咽癌组织中进行RT-PCR验证，通过另外3例鼻咽慢性炎症组织的表达谱比较，RT-PCR结果显示，CR2在41例（82%）鼻咽癌组织中表达下调，MLXIP在42例（84%）鼻咽癌组织中表达上调。我们在前期的研究中已经知道表达谱芯片的分析结果为，9个基因（q0.01）至少在17例（85%）鼻咽癌组织中有2倍差异表达，其中8个下调基因（TAOK3，SLC16A2，PRB4，AMY2B，B3GALT4，MSMB，RPS27，CR2），1个上调基因（MXLIP）。所以RT-PCR结果与芯片表达结果相同。 2、鼻咽癌组织中，CR2和MLXIP的表达与患者年龄、性别、临床分期、T分期及颈部淋巴结转移情况均无关（P0.05）。 3、本次研究结果显示ZNF608和CSF1R在鼻咽癌放射抗拒型组中表达上调，在鼻咽癌放射敏感型组中表达下调。在前期的研究中，ZNF608和CSF1R这两个基因在芯片结果中显示为，在鼻咽癌放射抗拒型病例组中表达上调，在鼻咽癌放射敏感型组中表达下调，它们的表达差异倍数分别是4.10倍和1.89倍。表明RT-PCR验证结果与芯片表达结果一致。 4、鼻咽癌组织中，ZNF608和CSF1R的表达与患者年龄、临床分期、T分期及颈部淋巴结转移情况均无关（P0.05）。结论： 1、与炎症组织相比较，CR2在41例（82%）鼻咽癌组织中表达下调，MXLIP在42例（84%）鼻咽癌组织中表达上调，与芯片表达结果一致，，CR2和MLXIP在鼻咽癌组织中有明显的差异表达，表明CR2和MLXIP在低分化鼻咽癌的诊断上有一定的作用。 2、鼻咽癌组织中，CR2和MLXIP的表达与患者年龄、性别、临床分期、T分期及颈部淋巴结转移情况无相关性。 3、ZNF608和CSF1R在鼻咽癌放射抗拒型病例组中表达上调，在鼻咽癌放射敏感型组中表达下调，与芯片表达结果一致，ZNF608和CSF1R有望作为预测鼻咽癌放射敏感性的指标。 4、鼻咽癌组织中，ZNF608和CSF1R的表达与患者年龄、临床分期、T分期及颈部淋巴结转移情况无相关性。
[Abstract]:Background & AIM: in previous studies, we compared the expression profiles of 20 nasopharyngeal carcinoma tissue samples with 15 nasopharyngeal chronic inflammation tissue samples by using cDNA microarrays capable of detecting 14112 human genes. To identify the genes that are differentially expressed in nasopharyngeal carcinoma (NPC) tissues. The purpose of this study was to select a complement receptor 2 (CR2) / MLX interacting protein (MLXIP) from 1 to 50 NPC tissues to verify whether it was consistent with the results of gene chip expression. 2. The radiosensitivity genes zinc finger protein 608 (ZNF608) and colony stimulating factor 1 receptor (CSF1R) were selected. RT-PCR was performed in 17 cases of nasopharyngeal carcinoma to verify whether the results were consistent with the results of gene chip expression. 3. To analyze the correlation between the expression of CR2 gene MLXIPP ZNF608 and CSF1R in nasopharyngeal carcinoma (NPC). Methods: the expression of CR2MLXIPNZNF608 and CSF1R in 50 cases of poorly differentiated nasopharyngeal carcinoma (NPC) and 17 cases of poorly differentiated nasopharyngeal carcinoma (NPC) were examined by real-time quantitative RT-PCR, and the correlation between CR2MLXIPNZNF608 and CSF1R was analyzed. Results: 1. CR2 and MXLIP were used for RT-PCR in 50 cases of poorly differentiated nasopharyngeal carcinoma. The results of RT-PCR showed that the expression of CR2 was down-regulated in 41 cases (82%) of nasopharyngeal carcinoma tissues, and the expression of MLXIP was up-regulated in 42 cases (84%) of nasopharyngeal carcinoma tissues. We have known in previous studies that 9 genes (q0.01) were twice differentially expressed in at least 17 cases (85%) of nasopharyngeal carcinoma (NPC), including 8 down-regulated genes (TAOK3, SLC16A2, PRB4, AMY2GALT4MMBRPS27CR2) and 1 up-regulated gene (MXLIP). So the results of RT-PCR and microarray expression were the same. 2. The expression of CR2 and MLXIP in nasopharyngeal carcinoma tissues was related to the age and sex of patients. Clinical staging T stage and cervical lymph node metastasis were not significant (P0.05). The results showed that the expression of ZNF608 and CSF1R was up-regulated in the radioresistance group of nasopharyngeal carcinoma and down-regulated in the radiosensitive group of nasopharyngeal carcinoma. In previous studies, two genes, ZNF608 and CSF1R, were found to be up-regulated in patients with nasopharyngeal carcinoma (NPC) and down-regulated in radiosensitive nasopharyngeal carcinoma (RRS). The differences were 4.10 and 1.89 times, respectively. The results of RT-PCR were consistent with the results of microarray expression. 4. The expression of ZNF608 and CSF1R in nasopharyngeal carcinoma was not correlated with age, clinical staging and cervical lymph node metastasis (P0.05). Conclusion: 1. Compared with inflammatory tissue, the expression of CR2 in 41 cases (82%) of nasopharyngeal carcinoma was down-regulated in 42 cases (84%) of nasopharyngeal carcinoma, which was consistent with the results of microarray expression. There was significant difference between CR2 and MLXIP in nasopharyngeal carcinoma. CR2 and MLXIP may play an important role in the diagnosis of poorly differentiated nasopharyngeal carcinoma. 2. The expression of CR2 and MLXIP in nasopharyngeal carcinoma is associated with age and sex. There was no correlation between clinical staging and cervical lymph node metastasis. 3The expression of ZNF608 and CSF1R was up-regulated in patients with radioresistance of nasopharyngeal carcinoma, but down-regulated in radiosensitive type of nasopharyngeal carcinoma. ZNF608 and CSF1R may be used as predictors of radiosensitivity of nasopharyngeal carcinoma. (4) there is no correlation between the expression of ZNF608 and CSF1R in nasopharyngeal carcinoma tissue and age, clinical stage, T stage and cervical lymph node metastasis.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.63

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