RASSF1A基因在喉鳞状细胞癌及喉角化症组织中的表达及其临床意义

发布时间：2018-06-27 07:29

本文选题：喉癌 + 喉角化症　；参考：《安徽医科大学》2012年硕士论文

【摘要】：目的喉鳞状细胞癌是指发生于喉黏膜上皮的恶性肿瘤，是耳鼻咽喉头颈外科最常见的恶性肿瘤之一。喉癌抑癌基因RASSF1A的异常表达与喉鳞状细胞癌的关系近年来国内鲜有报道。RASSF1A基因通过干预细胞周期、抑制细胞生长、促进细胞凋亡而抑制肿瘤的发生。该基因启动子发生甲基化之后会引起基因的失活，导致细胞周期紊乱而发生癌变。本研究观察喉鳞状细胞癌组织中的RASSF1A表达情况，探讨RASSF1A基因的表达与喉鳞状细胞癌的发生及病变进展的关系，从而有望在临床诊疗中使其成为一项新的诊断及预后评估的指标，以提高早期诊断率及肿瘤的治愈率。喉角化症是喉的癌前病变,是喉部黏膜上皮生长、成熟异常以及黏膜上皮过度角化的一种增生性疾病。RASSF1A基因在喉的癌前病变组织中的甲基化及表达方面研究甚少。本研究观察喉角化症组织中RASSF1A的表达情况，来初步探讨RASSF1A基因与喉角化症发病是否具有相关性。方法收集安徽省立耳鼻咽喉头颈外科自2009-2011年的术中切除的声带息肉、喉角化症及喉鳞状细胞癌组织，另外取尸头正常声带黏膜。用免疫组织化学方法检测各组组织的RASSF1A蛋白表达情况。RASSF1A蛋白经免疫组织化学方法染色后为棕黄色颗粒，在细胞浆内弥漫性分布，细胞核不着色。计算每例组织切片中RASSF1A阳性细胞百分数，取平均值±标准差(x±s)，采用SPSS17.0统计分析软件对数据进行分析，P＜0.05为差异有统计学意义。结果 RASSF1A在喉鳞状细胞癌及喉角化症组织中的表达低于声带息肉组织和正常喉黏膜组织；喉鳞状细胞癌与声带息肉，喉鳞状细胞癌与正常喉黏膜及喉鳞状细胞癌与喉角化症进行比较，RASSF1A表达差异均具有统计学意义(P0.05)。喉角化症与声带息肉比较，喉角化症与正常喉黏膜组织比较以及喉角化症各类型之间进行比较，RASSF1A表达差异不具有统计学意义(P0.05)。 RASSF1A在喉鳞状细胞癌临床I-IV期中的表达情况呈现逐渐下降趋势，各临床分期间差异具有统计学意义(P0.05)。RASSF1A在高分化、中分化及低分化癌组织中表达差异以及各年龄组之间表达的差异亦不具有统计学意(P0.05)。结论 1.喉鳞状细胞癌组织中RASSFlA蛋白的表达程度明显低于正常喉组织及声带息肉组织，提示RASSFlA蛋白表达降低与喉鳞状细胞癌的发生有关; 2.RASSFlA的表达与患者年龄及组织的病理学分化程度无关，但与喉鳞状细胞癌的临床分期有关，不同临床分期喉癌组织RASSFlA蛋白的表达程度不同，临床分期越早，RASSFlA蛋白表达水平越高;临床分期越晚，RASSFlA蛋白表达水平越低；提示RASSFlA的表达可能与肿瘤的浸润及转移有关。 3.RASSF1A基因可能参与了喉鳞状细胞癌发生的早期过程，因此可以利用此特征作为喉鳞状细胞癌的早期诊断指标，并且对于在鉴别癌前病变与喉癌时可能具有一定意义; 4.对RASSFlA蛋白表达进行检测，有助于筛查RASSFlA基因异常的人群，，提搞喉鳞状细胞癌的早期诊断水平。
[Abstract]:Purpose

The expression of RASSF1A gene in laryngeal squamous cell carcinoma and its relationship with laryngeal squamous cell carcinoma have been reported .

RASSF1A gene is a kind of proliferative disease in laryngeal carcinoma . The RASSF1A gene has been used to investigate the methylation and expression of RASSF1A gene in laryngeal carcinoma .

method

The expression of RASSF1A protein was detected by immunohistochemical method . RASSF1A protein was stained by immunohistochemistry . The percentage of RASSF1A positive cells in each group was calculated and the mean 卤 standard deviation ( x 卤 s ) was calculated . The data were analyzed by SPSS 17.0 statistical analysis software , and the difference was statistically significant .

Results

The expression of RASSF1A in laryngeal squamous cell carcinoma and laryngeal keratopathy was lower than that of vocal cord polyp and normal laryngeal mucosa .
The expression of RASSF1A in laryngeal squamous cell carcinoma and vocal cord polyp , laryngeal squamous cell carcinoma and normal laryngeal mucosa and laryngeal squamous cell carcinoma were statistically significant ( P0.05 ) .

There was no significant difference in RASSF1A expression compared with vocal cord polyp ( P0.05 ) .

RASSF1A showed a decreasing trend in the clinical I - IV period of laryngeal squamous cell carcinoma ( P0.05 ) . RASSF1A had no statistical significance in the expression of RASSF1A in high differentiation , middle differentiation and low differentiated cancer tissues and the difference between different age groups ( P0.05 ) .

Conclusion

1 . The expression of RASSFlA protein in laryngeal squamous cell carcinoma was significantly lower than that in normal laryngeal tissues and vocal cord polyp tissues , suggesting that RASSFlA protein expression was associated with the occurrence of laryngeal squamous cell carcinoma ;

2 . The expression of RASSFlA was not related to the age of the patient and the pathological differentiation degree of the tissues , but the expression level of RASSFlA protein was different from the clinical stage of laryngeal squamous cell carcinoma . The higher the expression level of RASSFlA protein in different clinical stages , the higher the expression level of RASSFlA protein , the lower the level of RASSFlA protein expression was .
It is suggested that the expression of RASSFlA may be related to invasion and metastasis of tumor .

3 . RASSF1A gene may be involved in the early stage of laryngeal squamous cell carcinoma , so it can be used as an early diagnostic index for laryngeal squamous cell carcinoma , and may be of certain significance in identifying precancerous lesions and laryngeal cancer ;

4 . The expression of RASSFlA protein is detected , which helps to screen the abnormal population of RASSFlA gene , which can be used for early diagnosis of laryngeal squamous cell carcinoma .
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.65

【参考文献】