白藜芦醇诱导Hep-2人喉表皮样癌细胞自噬作用

发布时间：2018-07-02 09:00

  本文选题：白藜芦醇 + 人喉表皮样癌细胞　； 参考：《医学理论与实践》2016年22期

【摘要】：目的:观察白藜芦醇对Hep-2细胞形态学的影响及诱导其自噬的可能机制。方法:40μM浓度白藜芦醇复合培养液作用于Hep-2细胞后,MDC染色和透射电镜分别检测自噬小体和自噬泡;Hoechest33258染色检测凋亡细胞;western blot分别分析自噬基因蛋白水平。结果:白藜芦醇增加LC3Ⅱ与LC3Ⅰ的比率,促进beclin1蛋白和mRNA的表达,细胞内的自噬泡和自噬小体数量也显著增加。用3MA和CQ抑制自噬则白藜芦醇对Hep-2细胞的促凋亡作用显著减弱。结论:白藜芦醇能够促进Hep-2细胞自噬;白藜芦醇诱导Hep-2细胞自噬的机制可能与促进LC3Ⅱ与LC3Ⅰ的表达、增强自噬基因beclin1蛋白的表达有关。
[Abstract]:Aim: to observe the effect of resveratrol on the morphology of Hep-2 cells and the possible mechanism of inducing autophagy. Methods Hep-2 cells were treated with resveratrol at the concentration of 40 渭 M respectively. The levels of autophagy gene protein in apoptotic cells were detected by Western blot staining and transmission electron microscopy (TEM), respectively. Results: resveratrol increased the ratio of LC3 鈪，

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