Testin基因对高转移鼻咽鳞状细胞癌的影响

发布时间：2018-07-02 13:38

本文选题：Testin基因 + 鳞状细胞癌　；参考：《临床耳鼻咽喉头颈外科杂志》2017年21期

【摘要】：目的:探讨Testin(TES)基因对人鼻咽鳞状细胞癌株5-8F细胞增殖、迁移的影响。方法:以RTPCR法从人鼻咽鳞状细胞癌株5-8F细胞获得目的基因,构建真核表达载体pEGFP-N1-TES,采用PCR、基因测序等对其进行鉴定;然后将其转导入人高转移鼻咽鳞状细胞癌株5-8F中,通过RT-PCR法及Western-blot法对其进行鉴定。接着采用流式细胞术、划痕试验检测转染后5-8F细胞增殖及迁移能力的影响。结果:转染外源性TES基因后,转染外源性TES基因的5-8F细胞呈现明显的凋亡,差异有统计学意义(P0.05)。细胞划痕实验表明:在第12、24、48h时,TES组细胞迁移速率明显比空载体组和未转染组要慢(P0.01)。结论:该研究成功建立了能稳定高表达TES的细胞模型。TES基因在体外可明显抑制鼻咽癌5-8F细胞的增殖和降低其迁移运动能力,可能是一种潜在的抑癌基因。
[Abstract]:Aim: to investigate the effect of Testin (tes) gene on proliferation and migration of human nasopharyngeal squamous cell carcinoma cell line 5-8F. Methods: the target gene was obtained from human nasopharyngeal squamous cell carcinoma cell line 5-8F by RT-PCR, and the eukaryotic expression vector pEGFP-N1-TESwas constructed and identified by PCRand gene sequencing, and then transferred into human nasopharyngeal squamous cell carcinoma cell line 5-8F with high metastasis. It was identified by RT-PCR and Western-blot. Flow cytometry and scratch test were used to detect the effect of transfection on the proliferation and migration of 5-8 F cells. Results: after transfection of exogenous tes gene, 5-8F cells transfected with exogenous tes gene showed obvious apoptosis (P0.05). The cell scratch test showed that the migration rate of cells in the tes group was slower than that in the empty vector group and the untransfected group (P0.01). Conclusion: this study successfully established a stable and highly expressed tes cell model. Tes gene can significantly inhibit the proliferation of nasopharyngeal carcinoma 5-8F cells and reduce its migration and motor ability in vitro. It may be a potential tumor suppressor gene.
【作者单位】：武汉大学中南医院耳鼻咽喉头颈外科;湖北省第三人民医院耳鼻咽喉科;
【基金】：湖北省自然科学基金资助项目(No:2009CBD420)
【分类号】：R739.63

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