芪菊袋泡茶药效学实验及治疗EB病毒感染的临床疗效观察

发布时间：2018-07-04 22:35

本文选题：芪菊袋泡茶 + EB病毒感染　；参考：《广州中医药大学》2012年硕士论文

【摘要】：背景 EB病毒是一种具有复杂基因组的人类疱疹病毒,全球90%以上的人受到过该病毒的感染。EB病毒对人类具有普遍易感性且与多种人类疾病尤其是恶性肿瘤疾病密切相关。EB病毒在人体内感染情况对于鼻咽癌(NPC)的诊断、临床分期、预后判断和监测放疗后转移复发等均具有重要意义。有研究者提出EB病毒是鼻咽癌的主要致病因素之一。EB病毒与鼻咽炎也有密切的相关性,研究发现EB病毒感染者较EB病毒阴性者有较高的鼻咽炎患病率。研究目的本研究通过免疫试验,客观评价芪菊袋泡茶药效学上增强小鼠非特异性免疫功能。通过对EB病毒感染的临床疗效观察,与对照组即未使用任何临床干预组作对比,观察芪菊袋泡茶对EB病毒感染的治疗效果,以期为中医药治疗EB病毒感染提供一种疗效肯定又确切可行的治疗手段EB病毒,对EB病毒感染的预防和治疗及提高中医药治疗在EB病毒感染治疗中的地位均具有重要意义。研究方法 1.药效学实验：将小鼠称重、编号后,随机分为实验组,标准对照组及空白对照组,其中实验组分为高剂量组,中剂量组,低剂量组。按实验方案灌胃给药,注射鸡红细胞悬液,处死小鼠,抽取腹腔液0.5ml,滴于干净的载玻片上,每片0.5ml,每只小白鼠2片。然后放在垫有湿纱布的搪瓷盒中,37℃孵育半小时。取出玻片自来水冲去贴壁的细胞,自然凉干。用丙酮-甲醇溶液(1：1)固定5分钟后取出。Giemsa-W right混合染色3～5分钟,自来水冲洗,晾干后,油镜观查计数200个巨噬细胞,计算吞噬指数(吞噬指数=被吞噬的鸡红细胞总数/200个巨噬细胞)和吞噬百分率(吞噬百分率=吞噬鸡红细胞的巨噬细胞数/200个巨噬细胞)。记录整理全部数据,用方差分析进行统计分析。 2.临床疗效观察：制定病例纳入标准和排除标准,将EB病毒感染或EB病毒感染合并气虚热毒鼻咽炎的患者采用查随机数字表后随机分配成两组,一组为芪菊袋泡茶治疗组,口服袋泡茶,一次2包,开水浸泡15分钟后饮服,一天2次；一组为空白对照组,即未进行任何临床干预组。分别在3周后复查EB病毒,观察EB病毒转阴情况。 3.统计分析：计数资料采用卡方检验,计量资料采用t检验,全部数据均输入计算机数据库,应用SPSS13.0统计软件进行统计分析,取检验水准为a=0.05。分别对两组患者前后的EB病毒转阴率进行对比,组间对比,比较两组间疗效。研究结果 1.药效学实验统计吞噬率(吞噬百分率=吞噬鸡红细胞的巨噬细胞数/200个巨噬细胞)及吞噬指数(吞噬指数=被吞噬的鸡红细胞总数/200个巨噬细胞),5组间通过方差分析,方差齐性检验P0.05,方差齐性,各组间有可比性。组间比较,P0.05,实验组、标准对照组与空白对照组之间吞噬率及吞噬指数的对比均有差别,实验组与标准对照组及空白对照组对比具有差异,中剂量组与高剂量组、低剂量组、标准对照组及空白对照组之间均有差异,具有统计学意义,表明芪菊袋泡茶有增强小鼠非特异性免疫功能。 2.临床疗效观察所有病例来源于2011年3月至2012年4月广州中医药大学第一附属医院耳鼻喉科门诊,共56例,随机分为两组,其中芪菊袋泡茶治疗组26例,18例合并有鼻咽炎相关症状,8例无合并症状。空白对照组30例,合并有鼻咽炎相关症状者为22例,无合并症状者为8例。按要求服药后随访复查。经统计学方法统计分析,对照组与治疗组在治疗前总积分、性别,年龄,烟酒嗜好等方面无差异,P0.05,两组间基本信息平衡,具有可比性。本次研究中治疗组26例患者服用芪菊袋泡茶期间未见明显不良反应,3周后复查血清EB病毒,其中有12例转阴,转阴率为46.2%,对照组复查血清EB病毒转阴的有5例,转阴率为16.7%,经卡方检验,比较两组间EB病毒感染转阴率,P0.05,具有统计学意义。治疗组18例合并有鼻咽炎相关症状者复查血清EB病毒9例转阴,转阴率为50%。8例无合并症状者转阴的有3例,转阴率为37.5%。对照组中合并有鼻咽炎相关症状的22例中3例转阴,转阴率为16.7%。8例无合并症状者转阴的有2例,转阴率为25.0%。经卡方检验,比较治疗组及对照组有症状及无症状者转阴情况,P0.05,无统计学意义。经t检验,两组间治疗后总积分差值、主症差值、次症差值及体征差值比较,均P0.01,具有显著性差异。研究结论芪菊袋泡茶在药效上有增强小鼠非特异性免疫功能的作用,能促进EB病毒转阴,并能改善EB病毒感染患者的临床症状。芪菊袋泡茶为纯中药制剂,安全性高,服药方便且易携带,可广泛推广于临床。
[Abstract]:background
EB virus is a human herpesvirus with a complex genome. More than 90% of the world has been infected with the virus, and the.EB virus has universal susceptibility to human beings and is closely related to a variety of human diseases, especially malignant tumor diseases. The diagnosis of nasopharyngeal carcinoma (NPC), clinical stage and prognosis of.EB virus infection in human body (NPC) Some researchers suggest that EB virus is one of the main pathogenic factors of nasopharyngeal carcinoma,.EB virus is also closely related to nasopharyngitis. The study found that EB virus infected people have higher incidence of nasopharyngitis than those with EB virus negative.
research objective
In this study, the non specific immune function of the mice was objectively evaluated through the immunological test. Through the observation of the clinical efficacy of EB virus infection and compared with the control group, that is, the treatment effect of Qi chrysanthemum tea bag tea to EB virus infection was observed in order to provide the treatment of EB virus infection by traditional Chinese medicine. It is of great significance for the prevention and treatment of EB virus infection and the improvement of the status of the treatment of Chinese medicine in the treatment of EB virus infection, which is an effective and feasible therapeutic method for the treatment of EB virus.
research method
1. pharmacodynamic experiments: the mice were weighed and numbered. The mice were randomly divided into experimental group, standard control group and blank control group. The experimental group was divided into high dose group, medium dose group and low dose group. The experimental group was administered by the experimental scheme, the chicken red cell suspension was injected, the mice were killed and the abdominal cavity liquid 0.5ml was extracted, dripping on the clean slides, each piece of 0.5ml, and each small tablet. 2 slices of rat. Then put in the enamel box with wet gauze, hatch for half an hour at 37. Take the glass tap water to wash the wall cells and dry. After 5 minutes of acetone methanol solution (1:1),.Giemsa-W right is taken out for 3~5 minutes, and the tap water is washed and dried, and 200 macrophages are counted and counted to calculate the phagocytic finger. The number (phagocytic index = /200 macrophage of the total number of chicken red cells being phagocytosis) and the percentage of phagocytosis (phagocytic percentage = /200 macrophages, macrophages phagocytosis of chicken red cells). All data were recorded and analyzed by variance analysis.
2. clinical effect observation: the cases were taken into the standard and exclusion criteria, and the patients with EB virus infection or EB virus infection combined with Qi deficiency and fever nasopharyngitis were randomly assigned into two groups, one group was Qi Ju tea bag treatment group, oral tea bag tea, one time 2 packs, 15 minutes after water immersion, 2 times a day; one group was empty. White control group, that is, no clinical intervention group. After 3 weeks, the EB virus was re examined and the EB virus negative condition was observed.
3. statistical analysis: counting data using chi square test, measurement data using t test, all the data are input to the computer database, SPSS13.0 statistical software is used for statistical analysis, the test level is a=0.05. for the two groups of patients before and after the EB virus conversion rate is compared, the comparison between groups, compare the two groups of curative effect.
Research results
1. pharmacodynamic experiments
Statistical phagocytosis rate (phagocytic percentage = /200 macrophage macrophages phagocytosis of chicken red cells) and phagocytic index (phagocytic index = /200 macrophages of the total number of chicken red cells being phagocytic). The 5 groups were analyzed by variance, Fang Chaqi test P0.05, homogeneity of variance, the comparability among the groups. P0.05, experimental group, standard control group There were differences in the comparison of phagocytic rate and phagocytic index between the control group and the blank control group. The comparison between the experimental group and the standard control group and the blank control group was different. There were differences between the middle dose group and the high dose group, the low dose group, the standard control group and the blank control group, with statistical meaning, indicating that the Qi chrysanthemum tea bag had the enhancement of the non specificity of the mice. Immunity.
2. clinical curative effect observation
All cases were from the Department of ENT of the First Affiliated Hospital of Guangzhou University of Chinese Medicine from March 2011 to April 2012. 56 cases were randomly divided into two groups, including 26 cases of Qi chrysanthemum tea bag treatment group, 18 cases with rhinopharyngitis related symptoms, 8 cases without amalgamative symptoms. 30 cases in the blank control group, 22 cases with nasopharyngitis related symptoms, without complication. According to the statistical analysis, there was no difference between the control group and the treatment group before the treatment. There was no difference between the control group and the treatment group before the treatment. P0.05, the basic information of the two groups was balanced and comparable. In this study, 26 patients in the treatment group had no obvious adverse reaction during the period of taking Qi chrysanthemum tea bags. 3 weeks after the reexamination of the serum EB virus, 12 of them turned Yin and the conversion rate was 46.2%. 5 cases of the control group rechecked the sera EB virus turned negative, and the negative rate was 16.7%. The negative rate of the EB virus infection between the two groups was compared by the chi square test, and the statistical significance was compared. 18 cases in the treatment group were combined with the nasopharyngitis related symptoms to recheck the serum EB virus 9 cases turn Yin and turn to the negative. There were 3 cases of negative rate of 50%.8 without symptomatic patients. The negative rate was 3 of the 22 cases in the 37.5%. control group with nasopharyngitis related symptoms in 3 cases, and the negative rate was 2 cases in 16.7%.8 without symptomatic patients, and the negative rate was 25.0%. through the chi square test. Statistical significance. After t test, the difference between the two groups in total integral difference, main difference, secondary difference and sign difference were all P0.01, with significant difference.
research conclusion
The Qi chrysanthemum tea bag can enhance the non specific immune function of the mice, can promote the EB virus to turn to the Yin, and can improve the clinical symptoms of the EB virus infected patients.
【学位授予单位】：广州中医药大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R273;R739.63

【参考文献】