眼球壁小梁网主要由平滑肌纤维构成且不存在内皮细胞

发布时间：2018-07-08 13:00

本文选题：小梁网 + 平滑肌　；参考：《南方医科大学学报》2017年10期

【摘要】：目的证明小梁网(TM)主要由平滑肌纤维而非胶原纤维构成,且不存在内皮细胞。方法选用家兔、SD大鼠、小鼠各3只,新鲜眼球共18只,沿矢状面对半切开眼球,石蜡包埋,常规组织切片,每个标本选取5片,分别采用HE染色、VG染色、Masson染色、α-SMA及CD31免疫组化法染色,显微镜下观察,并扫描成数字切片。将TM与巩膜胶原纤维、睫状肌(平滑肌)细胞、血管内皮细胞进行对比观察。结果 HE染色:3种动物TM呈紫红色粗丝状结构,交织成网,与睫状肌纤维胞质染色高度一致;TM表面附着的"细胞"界限不清,核扁,细长,呈深紫色,其"胞质"与TM无明显界限;睫状肌为典型的平滑肌,按不同方向成束排列,纵切的细胞呈扁平状,胞质紫红色,核扁,细长;巩膜内胶原纤维呈红色,深染;成纤维细胞少,依附于胶原纤维,其长轴与胶原纤维长轴一致,轮廓不清,核细长呈线样,深紫蓝色;血管内皮细胞胞质呈淡紫色,核深染,由于切面原因而呈不同的形状,突于腔内。VG染色:TM呈淡红色细丝状,胶原纤维呈亮红色粗条状。Masson染色:TM呈红绿相间网格状结构,其中以红色结构为主;巩膜胶原纤维呈绿色波浪状;血管内皮细胞呈绿色扁平状;睫状体的平滑肌纤维呈紫红色。α-SMA免疫组化:TM部分呈强阳性反应,胞质呈棕黄色颗粒;巩膜内胶原纤维及血管内皮细胞呈阴性;睫状体内的平滑肌纤维呈强阳性。CD31免疫组化:三种动物染色结果虽略有差异,但均为阴性。结论 TM主要由平滑肌纤维构成,外周包绕微量胶原纤维(肌内膜),不存在内皮细胞。
[Abstract]:Aim to demonstrate that the trabecular meshwork (TM) is mainly composed of smooth muscle fibers rather than collagen fibers, and no endothelial cells exist. Methods Sprague-Dawley rats (3 mice) and 18 fresh eyeballs were selected. The eyes were cut along the sagittal face, embedded in paraffin wax and sectioned with conventional tissue sections. Each specimen was divided into 5 pieces. Masson staining, 伪 -SMA and CD31 immunohistochemical staining were used for HE staining and VG staining, respectively, and were observed under microscope and scanned into digital sections. TM was compared with collagen fibers of sclera, ciliary muscle (smooth muscle) cells and vascular endothelial cells. Results HE staining showed that TM of three species of animals had a purplish red coarse filamentous structure and intertwined into a net. The boundary of "cells" attached to TM was not clear with the cytoplasmic staining of ciliary muscle fibers. The nucleus was flat, slender and dark purple. The ciliary muscle is a typical smooth muscle, arranged in bundles in different directions, with flattened cells, purplish red cytoplasm, flat nucleus, thin and long, deep stained collagen fibers in sclera, few fibroblasts, Attached to the collagen fiber, its long axis is consistent with the long axis of the collagen fiber, the outline is not clear, the nucleus is thin and line, dark purple, the vascular endothelial cell cytoplasm is lavender, the nucleus is deep stained, because of the cut plane reason takes on the different shape, the vascular endothelial cell cytoplasm appears the lavender, the nucleus is deep stained, VG staining in the lumen showed that: TM was light red filaments, and collagen fibers were bright red thick stripes. Masson staining showed red and green meshes, red structure was dominant, scleral collagen fibers were green and wavy. Vascular endothelial cells were green flattened, smooth muscle fibers of ciliary body were purplish red, 伪 -SMA immunohistochemistry showed strong positive reaction, cytoplasm was brown granules, collagen fibers and vascular endothelial cells in sclera were negative. The smooth muscle fibers in ciliary body were strongly positive. CD31 immunohistochemical staining: although the results of the three kinds of animal staining were slightly different, they were all negative. Conclusion TM is mainly composed of smooth muscle fibers.
【作者单位】：南方医科大学基础医学院//国家级医学基础实验教学示范中心//国家级医学形态学虚拟仿真实验教学中心;南方医科大学组织学与胚胎学教研室//国家级医学基础实验教学示范中心//国家级医学形态学虚拟仿真实验教学中心;南方医科大学学实验教学管理中心//国家级医学基础实验教学示范中心//国家级医学形态学虚拟仿真实验教学中心;
【分类号】：R775

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