AIFM1基因突变对Harlequin小鼠年龄相关耳蜗听觉功能的影响

发布时间：2018-07-08 19:35

本文选题：AIFM + 耳蜗电图　；参考：《中华耳科学杂志》2017年05期

【摘要】：目的研究AIFM1基因突变对Harlequin小鼠年龄相关耳蜗听觉功能的影响。方法用圆窗记录耳蜗电图来评价2月龄和6月龄野生型(WT)和AIFM1基因突变型(KO)Harlequin小鼠的耳蜗外周听觉功能,通过Phalloi-din荧光标记耳蜗基底膜外毛细胞的表皮板来评估毛细胞的损失程度。结果 (1)2月龄组WT型和KO型小鼠的复合动作电位阈值分别为18.6±1.6 d B SPL(n=6)和22.2±2.2 d B SPL(n=6),两组之间没有统计学差异(t=1.282,P0.05);6个月组WT型和KO型复合动作电位阈值分别为25.7±1.7 d B SPL(n=6)和42.1±3.6 d B SPL(n=6),两组之间差异有统计学意义t=4.129,P0.01)。(2)2月龄组WT型和KO型小鼠CAP的幅值没有统计学差异(P0.01),而6个月组的CAP的幅值在高频段(30,35和40 k Hz)有统计学差异(Two-way ANOVA,P0.05),在低频和中频则没有明显差异。2月龄组和6月龄组WT型和KO型小鼠10 k Hz以下CM的幅值的差异没有统计学意义(P0.05)。(3)耳蜗外毛细胞计数的结果显示,2月龄组的WT型(n=5)和KO型小鼠(n=5)之间没有统计学差异,而6月龄组的KO型小鼠的耳蜗外毛细胞个数在底回较WT型小鼠明显受损(t=15.1,P0.0001)。结论 AIFM1基因突变导致Harlequin小鼠耳蜗基底膜底回末端外毛细胞受损,从而导致耳蜗外周听觉功能高频段受损。
[Abstract]:Objective to study the effect of AIFM1 gene mutation on age-related auditory function in Harlequin mice. Methods the cochlear peripheral auditory function of wild-type (WT) and 6-month-old Harlequin mice (WT) and AIFM1 gene mutation (KO) were evaluated by electrocochleogram recorded by round window. The loss of hair cells was evaluated by Phalloi-din fluorescent labeled epidermis of the hair cells outside the basal membrane of the cochlea. Results (1) the threshold values of complex action potential of WT and KO type mice in 2-month group were 18.6 卤1.6 dB SPL (nong6) and 22.2 卤2.2 dB SPL (nong6), respectively. There was no significant difference between the two groups (t 1.282 P 0.05), and the complex action potential thresholds of WT type and KO type in 6-month group were 25.7 卤1.7 dB SPL (nong6), respectively. There was no significant difference between the two groups in the amplitude of CAP between WT and KO type mice (P0.01), but the amplitude of CAP in 6-month group had statistical difference in high frequency range (300.35 and 40kHz) (Two-way ANOVANALY P 0.05), but there was no significant difference in the amplitude of CAP between the two groups (P0.01). The difference of CAP amplitude between the two groups was significant (P0.01), but there was no significant difference in the amplitude of CAP between the two groups (P0.01), but there was no significant difference in the amplitude of CAP between the 6-month group and the 6-month group (P0.05). There was no significant difference in the amplitude of CM below 10 kHz between the WT and KO type mice of 2.2-month-old group and 6-month-old group (P0.05). (3). The results of cochlear hair cell count showed that WT type (nong5) and KO type in 2-month-old group were not significantly different from those in middle frequency group. There was no statistical difference between mice (nd5), However, the number of cochlear outer hair cells in 6-month-old KO mice was significantly damaged than that of WT mice at the bottom gyrus (tr 15.1, P 0.0001). Conclusion mutation of AIFM1 gene leads to damage of outer hair cells at the end of basal gyrus of cochlea in Harlequin mice, which results in high frequency damage of peripheral auditory function of cochlea.
【作者单位】：暨南大学附属珠海医院耳鼻咽喉头颈外科;听力与聋病中心纽约州立布法罗大学;中国人民解放军总医院耳鼻咽喉头颈外科;
【基金】：珠海市科技计划项目(20171009E030055)~~
【分类号】：R764

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