鼻息肉的全基因表达谱

发布时间：2018-07-12 10:50

本文选题：鼻息肉 + 基因芯片　；参考：《中国医科大学学报》2017年10期

【摘要】：目的研究鼻息肉的全基因表达谱情况,探寻其与正常鼻腔黏膜的基因表达差异。方法提取鼻息肉中的RNA,将RNA合成双链的c DNA,将标记的c DNA在Roche Nimble Gen基因芯片上进行杂交。应用Axon Gene Pix 4000B scanner进行扫描,应用Nimble Scan软件进行图像分析,探针及基因水平标准化后进行计算。结果与正常鼻腔黏膜相比,鼻息肉中有1 000个基因表达上调,占2.22%(1 000/45 033),有1 123个基因表达下调,占2.49%(1 123/45 033)。经过信号通路分析,发现在核糖体、蛋白酶体、柠檬酸循环、膀胱癌、非小细胞肺癌、神经胶质瘤、子宫内膜癌、内质网蛋白合成、慢性髓样白血病、谷胱甘肽代谢等信号通路中相关基因表达上调,在嗅觉传导、自然杀伤细胞介导的细胞毒性、细胞色素P450的化合物代谢、细胞色素P450的药物代谢、抗原加工和呈递、疟疾、移植物抗宿主疾病、视黄醇代谢、亚油酸代谢、戊糖和葡萄糖醛酸转换等信号通路中相关基因表达下调。结论鼻息肉的发生、发展与多个基因及信号通路的表达升高或降低有关,鼻息肉全基因表达谱的研究为探求鼻息肉的发生机制提供了有益的线索。
[Abstract]:Objective to study the gene expression profile of nasal polyp and to explore the difference between the gene expression of nasal polyp and that of normal nasal mucosa. Methods RNA was extracted from nasal polyps and synthesized into double-stranded cDNA. The labeled cDNA was hybridized on Roche Nimble Gen gene chip. Axon Gene Pix4000B scanner was used to scan, and Nimble scan software was used to analyze the image. The probe and gene level were standardized and calculated. Results compared with normal nasal mucosa, 1 000 genes were up-regulated in nasal polyps (2.22%) (1 000 / 45 033), and 1 123 genes were down-regulated (2 49%) (1 123 / 45 033). After signal pathway analysis, we found ribosome, proteasome, citric acid cycle, bladder cancer, non-small cell lung cancer, glioma, endometrial carcinoma, endoplasmic reticulum protein synthesis, chronic myeloid leukemia, Up-regulated gene expression in signaling pathways such as glutathione metabolism, transduction of olfactory cells, cytotoxicity mediated by natural killer cells, metabolism of cytochrome P450 compounds, drug metabolism of cytochrome P450, antigen processing and presentation, malaria, GVHD, retinol metabolism, linoleic acid metabolism, pentose and glucuronic acid conversion were down-regulated. Conclusion the development of nasal polyps is related to the increase or decrease of the expression of multiple genes and signal pathways. The study of the whole gene expression profile of nasal polyps provides a useful clue for exploring the pathogenesis of nasal polyps.
【作者单位】：中国医科大学附属盛京医院耳鼻咽喉科;
【基金】：辽宁省科学事业公益研究基金(2013001010) 沈阳市科学技术计划(F11-264-1-07,F13-220-9-21)
【分类号】：R765.25

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