细胞质胸苷激酶与喉癌及中医证型相关性研究

发布时间：2018-07-20 15:15

【摘要】：目的：观察血清TK1的浓度在喉癌及不同证型中的相关性。方法：于2010.03-2011.12期间按中医诊断标准先后收集病理确诊喉部鳞状细胞癌患者痰热壅滞型和气滞血瘀型各15例,共30例喉癌病例作为病例组,另收集30例喉部良性病变组作为对照组,病例组中分为痰热壅滞型组和气滞血瘀型组两组各15例。采用免疫印迹增强化学发光法对病例组和对照组进行血清中TK1浓度测定。收集各个实验对象的TK1浓度值,SPSS16.0进行数据统计分析,得出实验临床结果。结果：1病例组血清中的TK1浓度2.68(1.18-7.21)pmol/L明显高于对照组血清中的TKI浓度1.05(0.03-2.50)pmol/L；2.按中医辨证喉菌患者气滞血瘀型血清TK1浓度3.51(1.98-7.21)pmol/L较痰热壅滞型1.84(1.18-3.88)pmol／高；3.以TK1浓度大于2pmol/L为阳性判断标准,TK1诊断喉癌的灵敏度为60.00%,特异度为93.70%。表明TKI对喉癌诊断具有一定的临床意义(详见图1)。结论：血清TK1检测在喉癌患者及不同证型之间的临床诊断中具有临床意义。证实TK1浓度的检测对喉部恶性肿瘤诊断有相关性。
[Abstract]:Objective: to observe the correlation of serum TK 1 concentration in laryngeal carcinoma and different syndromes. Methods: according to traditional Chinese medicine diagnostic criteria, 15 cases of phlegm and heat stagnation type and 15 cases of qi stagnation and blood stasis type were collected from 30 cases of laryngeal carcinoma as case group and 30 cases of benign laryngeal lesions as control group. The patients were divided into two groups: phlegm and heat stagnation group and qi stagnation and blood stasis group. The concentration of TK1 in serum was determined by Western blotting enhanced chemiluminescence assay in the case group and the control group. The TK1 concentration of each experimental object was collected and SPSS 16.0 was collected for statistical analysis, and the clinical results were obtained. Results the serum TK1 concentration in the case of 1: 1 was 2.68 (1.18-7.21) pmol / L significantly higher than that in the control group (1.05 (0.03-2.50) pmol / L ~ (2). According to TCM syndrome differentiation, the serum TK1 concentration of patients with qi stagnation and blood stasis was 3.51 (1.98-7.21) pmol / L higher than that of phlegm-heat stagnation 1.84 (1.18-3.88) pmol/. The sensitivity and specificity of TK1 for the diagnosis of laryngeal carcinoma were 60.00and 93.70 respectively. This indicates that TKI has some clinical significance in the diagnosis of laryngeal carcinoma (see figure 1 for details). Conclusion: the detection of serum TK 1 has clinical significance in the diagnosis of laryngeal cancer patients and different syndromes. It was confirmed that the detection of TK 1 concentration was related to the diagnosis of laryngeal malignant tumor.
【学位授予单位】：福建中医药大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R273;R739.65

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