ALOX15在慢性鼻窦炎伴鼻息肉中表达及糖皮质激素对其作用研究

发布时间：2018-07-27 18:50

【摘要】：目的研究花生四烯酸酯15-脂氧合酶(arachidonate 15-lipoxygenase,ALOX15)在嗜酸粒细胞型慢性鼻窦炎伴鼻息肉(eosinophilic chronic rhinosinusitis with nasal polyps,ECRSw NP)及非嗜酸粒细胞型慢性鼻窦炎伴鼻息肉(nonesinophilc chronic rhinosinusitis with nasal polyps,non ECRSw NP)中表达,并研究口服糖皮质激素对其表达的影响。方法所有标本均由首都医科大学附属北京同仁医院标本库中获得,采用real-time PCR及免疫组织化学染色研究ALOX15表达与蛋白定位。比较激素治疗前ECRSw NP、non ECRSw NP及健康对照组中ALOX15 mRNA表达水平、蛋白定位及表达,并比较同一病人激素治疗前后mRNA及蛋白表达分布差异。结果 ECRSw NP组中ALOX15 mRNA及蛋白表达量高于健康对照组及non ECRSw NP组,差异有统计学意义(均P=0.000)。健康对照组、non ECRSw NP组间差异无统计学意义(P=0.594,P=0.550)。ALOX15蛋白表达于上皮细胞、浆细胞、中性粒细胞及嗜酸性粒细胞等多种细胞。ECRSw NP组激素治疗前后ALOX15 mRNA和蛋白表达差异有统计学意义(P=0.004,P=0.024)。结论 ALOX15在ECRSw NP中表达显著高于non ECRSw NP及健康对照组,口服糖皮质激素1周能抑制其表达。
[Abstract]:Objective to investigate the expression of arachidonate 15-lipoxygenase (ALOX15) in chronic eosinophil sinusitis with (eosinophilic chronic rhinosinusitis with nasal polypsm ECRSw NP and non-eosinophil chronic sinusitis with (nonesinophilc chronic rhinosinusitis with nasal polypsnon ECRSw NP). And to study the effect of oral glucocorticoid on its expression. Methods all specimens were collected from the specimen bank of Tongren Hospital affiliated to Capital Medical University. ALOX15 expression and protein localization were studied by real-time PCR and immunohistochemical staining. The expression of ALOX15 mRNA, protein localization and protein expression in ECRSw NPnon ECRSw NP and healthy control group were compared before and after hormone therapy, and the differences of mRNA and protein expression before and after hormone therapy in the same patient were compared. Results the expression of ALOX15 mRNA and protein in ECRSw NP group was significantly higher than that in healthy control group and non ECRSw NP group (P < 0. 000). The expression of ALOX15 protein in epithelial cells, plasma cells, neutrophils and eosinophils was not significantly different between the control group and control group (P0. 594P0. 550). The expression of ALOX15 mRNA and protein in ECRSw NP group was significantly different before and after hormone treatment (P0. 004, P0. 024). Conclusion the expression of ALOX15 in ECRSw NP is significantly higher than that in non ECRSw NP and healthy control group. Glucocorticoid can inhibit the expression of ALOX15 in ECRSw NP for 1 week.
【作者单位】： 北京市耳鼻咽喉科研究所北京市鼻病重点实验室教育部耳鼻咽喉头颈外科重点实验室;首都医科大学附属北京同仁医院耳鼻咽喉头颈外科;
【基金】：国家自然科学基金重点项目(81630023,81400444,81470678,81420108009) 北京市医院管理局“使命”计划(SML20150203),北京市医院管理局“青苗”计划(QML20150202) 首都临床特色应用研究(Z141107002514122) 首都卫生发展科研专项重点攻关项目(2016-1-2052) 北京市“百千万”人才项目(2016)~~
【分类号】：R765.41
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本文编号：2148816