色素上皮衍生因子与炎症的相关性研究

发布时间：2018-07-29 13:29

【摘要】：目的：了解多囊卵巢综合征（PCOS）妇女血清中色素上皮衍生因子（PEDF）与超敏C反应蛋白（hs-CRP）的关系。方法：纳入96例PCOS妇女，同时选取63例年龄匹配的正常女性作对照。测量身体参数（如身高、体重等）；测定空腹血清PEDF、 hs-CRP、补体C3（C3）、血脂、胰岛素、总睾酮等。所有的PCOS妇女和对照中的20例女性接受高胰岛素—正葡萄糖钳夹术以了解胰岛素的敏感程度。结果：（1）PCOS组血清中PEDF和hs-CRP水平较对照组明显升高。（2）Pearson相关分析显示，PCOS组血清PEDF水平与hs-CRP、C3、体重指数（BMI）、腰围、收缩压（SBP）、空腹血清胰岛素、甘油三脂、总胆固醇、低密度脂蛋白胆固醇（LDL-c）呈正相关，与高密度脂蛋白胆固醇和M值呈负相关。校正BMI后的偏相关分析显示，，PCOS组血清PEDF水平仍与hs-CRP呈正相关。（3）进一步行逐步多元线性回归分析后结果显示，hs-CRP、SBP、LDL-c和M值是PCOS患者血清PEDF水平的独立影响因素。结论：PCOS妇女血清中PEDF水平与hs-CRP正相关，提示PEDF与PCOS患者慢性炎症关系密切。目的：观察肿瘤坏死因子-α（TNF-α）对3T3-L1脂肪细胞表达和分泌色素上皮衍生因子（PEDF）的调节作用。方法：体外培养和诱导分化3T3-L1前脂肪细胞。分别用不同浓度TNF-α（0.1,1,10,100ng/ml）处理3T3-L1脂肪细胞24h，并选取10ng/ml的TNF-α干预细胞3、6、12、24h，采用实时荧光定量PCR和ELISA技术检测3T3-L1脂肪细胞PEDF的mRNA表达和蛋白分泌情况。结果：（1）和对照组相比，不同浓度TNF-α对3T3-L1脂肪细胞PEDF的mRNA表达和分泌均有抑制作用，且浓度越大，其抑制效应越明显（P0.05）。（2）10ng/ml的TNF-α干预3T3-L1脂肪细胞后，随着时间的延长，PEDF的mRNA表达逐渐降低，第12h达到最低点（P0.05）。（3）10ng/ml的TNF-α对其PEDF分泌的抑制率在第6h为15%，在第24h为33%（P0.05）。结论：TNF-α抑制3T3-L1脂肪细胞PEDF的表达和分泌，其抑制效应呈剂量和时间依赖性。
[Abstract]:Aim: to investigate the relationship between serum pigment epithelium-derived factor (PEDF) and hypersensitive C-reactive protein (hs-CRP) in women with polycystic ovary syndrome (PCOS). Methods: 96 PCOS women and 63 age-matched normal women were included as controls. Body parameters (such as height, weight), fasting serum PEDF, hs-CRP, complement C3 (C3), serum lipids, insulin, total testosterone and so on were measured. All PCOS women and 20 women in the control group received hyperinsulinemia-glucose clamp to understand insulin sensitivity. Results: (1) the levels of PEDF and hs-CRP in serum of PCOS group were significantly higher than those of control group. (2) the correlation analysis of Pearson showed that the serum PEDF level and hs-CRPPC C3, body mass index (BMI),) waist circumference, fasting serum insulin, triglyceride, total cholesterol, systolic blood pressure (SBP), fasting serum insulin, triglyceride, total cholesterol in PCOS group were significantly higher than those in control group. Low density lipoprotein cholesterol (LDL-c) was positively correlated with high density lipoprotein cholesterol and M value. The partial correlation analysis after correcting BMI showed that the serum PEDF level was still positively correlated with hs-CRP. (3) the results of stepwise multivariate linear regression analysis showed that the LDL-c and M values of PCOS patients were independent influencing factors of serum PEDF level. Conclusion there is a positive correlation between serum PEDF level and hs-CRP in women with PCOS, suggesting that PEDF is closely related to chronic inflammation in PCOS patients. Aim: to investigate the effect of tumor necrosis factor- 伪 (TNF- 伪) on the expression and secretion of pigment epithelium-derived factor (PEDF) in 3T3-L1 adipocytes. Methods: 3T3-L1 preadipocytes were cultured and induced in vitro. 3T3-L1 adipocytes were treated with different concentrations of TNF- 伪 for 24 h. TNF- 伪 of 10ng/ml was used to treat the adipocytes for 24 h. The mRNA expression and protein secretion of 3T3-L1 adipocytes were detected by real-time quantitative PCR and ELISA techniques. Results: (1) compared with the control group, different concentrations of TNF- 伪 inhibited the expression and secretion of PEDF in 3T3-L1 adipocytes, and the higher the concentration of TNF- 伪, the more obvious the inhibitory effect (P0.05) of 10ng/ml 10ng/ml on 3T3-L1 adipocytes. The expression of mRNA decreased gradually with time, and the inhibition rate of TNF- 伪 of 10ng/ml on PEDF secretion reached the lowest point (P0.05). (3) at the 12th hour (P0.05), and the inhibition rate of TNF- 伪 on PEDF secretion was 15 at 6 h and 33% at 24 h (P0.05). Conclusion: TNF- 伪 inhibits the expression and secretion of PEDF in 3T3-L1 adipocytes in a dose-and time-dependent manner.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R774.1

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